Growth suppression of human colorectal carcinoma in nude mice by monoclonal antibody C27-abrin A chain conjugate

Tsai, Li-Ping; Chen, Yung‐Liang; Lee, Chung; Chen, Horng-Min; Chang, Zo-Nan; Hung, Mei-Whey; Chao, Pei-Ling; Lin, Jeong‐Shi

doi:10.1007/bf02133980

Cited by 11 publications

(1 citation statement)

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“…Abrin is a natural toxic protein isolated from the seeds of Abrus precatorius L. and is one of the most powerful phytotoxins ever discovered. 1 Abrin comprises four isotoxic proteins, abrin-a, -b, -c, and -d. Of these, abrin-a is the most toxic. Abrin-a comprises two polypeptide chains (A and B) linked by a single disulfide bond.…”

Section: ' Introductionmentioning

confidence: 99%

Preparation and Identification of Monoclonal Antibody against Abrin-a

Yang

Zhang

et al. 2011

J. Agric. Food Chem.

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BALB/c mice were immunized four times with formalin-prepared abrin-a. Using the polyethylene glycol method, immunized splenocytes were isolated and fused with SP2/0 cells. An indirect ELISA was established and used to detect positive clones secreting monoclonal antibodies (mAbs) against abrin-a. After analysis, three hybridoma clones secreting IgG-subtype mAbs were obtained. The antibodies were purified from the hybridoma growth medium using protein A or G affinity chromatography. Western blot analysis was used to analyze the antigenic epitopes on abrin-a recognized by the mAbs. The mAbs were specific for abrin-a, with no detectable cross-reactivity with several homologous toxins and associated agglutinins. Sandwich ELISA was then developed using these mAbs, which had a detection limit for abrin-a of 7.8 ng/mL.

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Section: ' Introductionmentioning

confidence: 99%