Growth yield increase linked to caffeate reduction in Acetobacterium woodii

Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system

“…10 mM NaClO,, (v) 10 mM Na, SO, or (vi) (Tschech & Pfennig, 1984), 0.5 mg resazurin as redox indicator and 0.4 g Na,S as reducing agent. The medium was prepared under a N,/CO, atmosphere (80 : 20, v/v).…”

Sequence of subunit c of the Na+‐translocating F1FO ATPase of Acetobacterium woodii: proposal for determinants of Na+ specificity as revealed by sequence comparisons

“…woodii (DSM 1030) was obtained from the Deutsche Sammlung fur Mikro-organismen und Zellkulturen, Braunschweig, Germany, and grown under strictly anaerobic conditions on carbonate-buffered medium supplied with 0.4% glycine [9]. E. coli DH5a (supEAA A/acU169(O80/acZAM15) hsdrXl recAl endAl gyrA96 thil relAl [10]) was grown on LB at 37°C.…”

Novosphingobium acidiphilum sp. nov., an acidophilic salt-sensitive bacterium isolated from the humic acid-rich Lake Grosse Fuchskuhle

“…7H 2 O, 100 mM CaCl 2 . 2H 2 O and 250 mM CaCO 3 , 1 ml l 21 of SL 10 trace elements (Tschech & Pfennig, 1984) and 10 ml l 21 vitamin solution (Balch et al, 1979) buffered with 10 mM HEPES (pH 7.2) and supplemented with 0.001 % (v/v) Tween 20, a mixture of six amino acids (L-cysteine, L-alanine, L-isoleucine, Lleucine, L-asparagine and L-arginine, each 200 mM), a fatty acid mixture of formate, acetate and propionate (each 200 mM), as well as with glucose, pyruvate, citrate, 2-oxoglutarate and succinate (each 200 mM) (Jaspers et al, 2001). After 8 weeks incubation at room temperature in the dark, the novel strain was isolated from one of the highest positive dilution steps by streaking subsamples on YPG agar plates [1.2 % agar supplemented with a 0.22 mm filter-sterilized concentrate of yeast extract, peptone and glucose with final concentrations of 0.075, 0.15 and 0.075 % (w/v), respectively].…”