Guanylate-binding protein 6 is a novel biomarker for tumorigenesis and prognosis in tongue squamous cell carcinoma

Liu, Pei‐Feng; Chen, Hung-Chih; Shu, Chih‐Wen; Sie, Huei-Cin; Lee, Cheng-Hsin; Liou, Horng-Huei; Cheng, Jiin‐Tsuey; Ger, Luo‐Ping

doi:10.1007/s00784-019-03129-y

Cited by 14 publications

(12 citation statements)

References 41 publications

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“…Moreover, higher GBP1-6 expression is associated with better OS in skin cutaneous melanoma patients [33]. Furthermore, the gene expression level of GBP6 is significantly lower in OSCC patients with poor differentiation/lymph node metastasis, and low expression of GBP6 is associated with poor prognosis in TSCC patients [11]. Regarding current studies of GBP5 in cancers, a truncated splice variant of GBP5 was found in lymphoma [34].…”

Section: Discussionmentioning

confidence: 92%

“…The extracted RNA samples were then subjected to NGS analysis. More detailed descriptions about LCM and NGS analysis are available in our previous study [11].…”

Section: Laser Capture Microdissection (Lcm) and Next Generation Sequencing (Ngs) Of Lcm-captured Cellsmentioning

confidence: 99%

“…High-throughput profiling techniques such as RNA-sequencing (RNA-seq) using next-generation sequencing (NGS) [9] and RNAi high-throughput screening (HTS) [10] have been used to identify gene expression associated with many diseases such as cancer, and could provide a very powerful tool for discovering cancer biomarkers and therapeutic targets, especially for OSCC. For example, we have identified GBP6 as a favorable cancer biomarker in TSCC by NGS [11].…”

Section: Introductionmentioning

confidence: 99%

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Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma

Liu

Shu

Lee

et al. 2021

Cancers

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Guanylate binding protein 5 (GBP5) is the interferon (IFN)-inducible subfamily of guanosine triphosphatases (GTPases) and is involved in pathogen defense. However, the role played by GBP5 in cancer development, especially in oral squamous cell carcinoma (OSCC), is still unknown. Herein, next-generation sequencing analysis showed that the gene expression levels of GBP5 were significantly higher in OSCC tissues compared with those found in corresponding tumor adjacent normal tissues (CTAN) from two pairs of OSCC patients. Higher gene expression levels of GBP5 were also found in tumor tissues of 23 buccal mucosal squamous cell carcinoma (BMSCC)/14 tongue squamous cell carcinoma (TSCC) patients and 30 oral cancer patients from The Cancer Genome Atlas (TCGA) database compared with those in CTAN tissues. Immunohistochemical results showed that protein expression levels of GBP5 were also higher in the tumor tissues of 353 OSCC patients including 117 BMSCC, 187 TSCC, and 49 lip squamous cell carcinoma patients. Moreover, TCGA database analysis indicated that high gene expression levels of GBP5 were associated with poor overall survival in oral cancer patients with moderate/poor cell differentiation, and associated with poor disease-free survival in oral cancer patients with moderate/poor cell differentiation and lymph node metastasis. Furthermore, GBP5-knockdowned cells exhibited decreased cell growth, arrest at G1 phase, and decreased invasion/migration. The gene expression of markers for epithelial−mesenchymal transition and cancer stemness was also reduced in GBP5-silenced oral cancer cells. Taken together, GBP5 might be a potential biomarker and therapeutic target for OSCC patients, especially for those with poor cell differentiation and lymph node metastasis.

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Section: Discussionmentioning

confidence: 92%

“…The extracted RNA samples were then subjected to NGS analysis. More detailed descriptions about LCM and NGS analysis are available in our previous study [11].…”

Section: Laser Capture Microdissection (Lcm) and Next Generation Sequencing (Ngs) Of Lcm-captured Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma

Liu

Shu

Lee

et al. 2021

Cancers

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“…In our research, we found that hsa-miR-1827 regulated the seven upregulated target genes, including GBP6, CEACAM8, C7orf34, KCNV2, OLFM4, NR5A1, and ZNF488. Studies have demonstrated that hsa-miR-1827 and its target(s) are closely associated with breast cancer, cervical cancer, and tongue squamous cell carcinoma [42][43][44][45][46][47][48]; we also explored whether these genes or miRNAs are involved in IDD, and then we further verify the connection of circRNA and IDD by constructing an integrated circRNA-miRNA-mRNA network. For example, the high expression of target genes CEACAM8 and OLFM4 plays a crucial role in the in ammatory process by increasing the level of in ammatory cytokines [49,50].…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of a circRNA-miRNA-mRNA Axis in Intervertebral Disc Degeneration

Xiong¹,

Tu²,

Gao³

et al. 2020

Preprint

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Background: Low back pain (LBP) is a common symptom in daily life and one of the primary causes is intervertebral disc degeneration (IDD). Growing studies have indicated that circular RNAs (circRNAs) are intimately associated with IDD; however, the underlying mechanism has not yet been elucidated. We aimed to explore how circRNAs regulate IDD in an effort to provide novel insight for clinical diagnosis and treatment. Methods: The sequencing data of circRNAs, microRNAs (miRNAs), and mRNA were acquired from Gene Expression Omnibus (GEO) datasets. By analyzing the dataset consisting of a control group and degenerated group, differentially expressed circRNAs, miRNAs, and mRNAs were collected, and then the intersection of circRNAs, miRNAs, and mRNAs was screened. According to these intersectional RNAs, we constructed an integrally circRNA-miRNA-mRNA network. Finally, using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, we further clarified functions of the intersectional mRNA in IDD. Results: we obtained 620 differentially expressed circRNAs(DEcircRNAs), 13 miRNA (DEmiRNA), 273 mRNAs(DEmRNAs), 12 intersectional miRNAs, and 47 intersectional mRNAs. Finally, based on interactional 8 circRNA, 5 miRNAs and 15 mRNAs, an integrally circRNA-miRNA-mRNA network was constructed. Eight circRNAs, contained hsa_circ_0032254, hsa_circ_0003183, hsa_circ_0032253, hsa_circ_0001293, hsa_circ_0004565, hsa_circ_0091570, hsa_circ_0077526, and hsa_circ_0057552, may regulate IDD onset and progression by acting as competing endogenous RNAs. The results of GO and KEGG analyses implied that the targeted genes might significantly correlate to IDD.Conclusion: our findings improved a better understanding of the circRNA-related ceRNA regulatory mechanism in IDD and offered possible targets for IDD treatment.

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“…However, GBP1 inhibits the proliferation, migration, and invasion of CRC cells by mediating the anti-tumor effect of IFN- γ ( Britzen-Laurent et al, 2013 ). Low GBP6 expression has been confirmed in tongue squamous cell carcinoma and is related to the poor prognosis of these patients ( Liu et al, 2020 ). There are no reports on the role of GBP-2 in PAAD to date.…”

Section: Introductionmentioning

confidence: 99%

GBP2 as a potential prognostic biomarker in pancreatic adenocarcinoma

Liu

Huang

et al. 2021

PeerJ

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Background Pancreatic adenocarcinoma (PAAD) is a disease with atypical symptoms, an unfavorable response to therapy, and a poor outcome. Abnormal guanylate-binding proteins (GBPs) play an important role in the host’s defense against viral infection and may be related to carcinogenesis. In this study, we sought to determine the relationship between GBP2 expression and phenotype in patients with PAAD and explored the possible underlying biological mechanism. Method We analyzed the expression of GBP2 in PAAD tissues using a multiple gene expression database and a cohort of 42 PAAD patients. We evaluated GBP2’s prognostic value using Kaplan–Meier analysis and the Cox regression model. GO and KEGG enrichment analysis, co-expression analysis, and GSEA were performed to illustrate the possible underlying biological mechanism. CIBERSORT and the relative expression of immune checkpoints were used to estimate the relationship between GBP2 expression and tumor immunology. Result GBP2 was remarkably overexpressed in PAAD tissue. The overexpression of GBP2 was correlated with an advanced T stage and poor overall survival (OS) and GBP2 expression was an independent risk factor for OS in PAAD patients. Functional analysis demonstrated that positively co-expressed genes of GBP2 were closely associated with pathways in cancer and the NOD-like receptor signaling pathway. Most of the characteristic immune checkpoints, including PDCD1, PDCDL1, CTLA4, CD80, TIGIT, LAG3, IDO2, and VISTA, were significantly expressed in the high-GBP2 expression group compared with the low-GBP2 expression group. Conclusion GBP2 acted as a potential prognostic biomarker and was associated with immune infiltration and the expression of immune checkpoints in PAAD.

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Guanylate-binding protein 6 is a novel biomarker for tumorigenesis and prognosis in tongue squamous cell carcinoma

Cited by 14 publications

References 41 publications

Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma

Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma

Integrated Analysis of a circRNA-miRNA-mRNA Axis in Intervertebral Disc Degeneration

GBP2 as a potential prognostic biomarker in pancreatic adenocarcinoma

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