Guided bone regeneration produced by new mineralized and reticulated collagen membranes in critical-sized rat calvarial defects

Abstract

The aim of this study was to evaluate the bone regenerative effect of glutaraldehyde (GA) cross-linking on mineralized polyanionic collagen membranes in critical-sized defects on rat calvarias. Bone calvarial defects were induced in Wistar rats, which were then divided into five groups: a sham group; a control group, which received a commercial membrane; and GA, 25GA, and 75GA groups, which received one of three different polyanionic collagen membranes mineralized by 0, 25, or 75 hydroxyapatite cycles and then cross-linked by GA. Bone formation was evaluated based on digital radiography and computerized tomography. Histological analyses were performed 4 and 12 weeks after the surgical procedure to observe bone formation, membrane resorption, and fibrous tissue surrounding the membranes. Measurement of myeloperoxidase activity, tumor necrosis factor alpha, and interleukin 1beta production was performed 24 h after surgery. The percentage of new bone formation in the GA, 25GA, and 75GA groups was higher compared with the control and sham groups. In the GA and 25 GA groups, the membranes were still in place and were contained in a thick fibrous capsule after 12 weeks. No significant difference was found among the groups regarding myeloperoxidase activity and interleukin 1beta levels, although the GA, 25GA, and 75GA groups presented decreased levels of tumor necrosis factor alpha compared with the control group. These new GA cross-linked membranes accelerated bone healing of the calvarium defects and did not induce inflammation. In addition, unlike the control membrane, the experimental membranes were not absorbed during the analyzed period, so they may offer advantages in large bone defects where prolonged membrane barrier functions are desirable.

VISSA-PLS-DA-Based Metabolomics Reveals a Multitargeted Mechanism of Traditional Chinese Medicine for Traumatic Brain Injury

“…Treatment with XFZY attenuated this upregulation, suggesting that the neuroprotective effect of XFZY against TBI may involve functional improvement of the BBB. However, bone resorption after skull fracture also results in the release of collagens (Verissimo et al., 2015), causing an increase in trans -4-hydroxy-L-proline level. The decreased trans -4-hydroxy-L-proline induced by XFZY may also result from healing of the traumatic skull.…”

Guided bone regeneration: materials and biological mechanisms revisited

“…Hydroxyapatite‐incorporated membranes have been shown to promote the functional activity of stromal cells and osteoblast‐like cells in vitro (Table ) and induce bone formation in vivo , in combination with non‐resorbable (Table ) and resorbable (Table ) membranes. The HA powder used for preparation of pure ceramic membrane or other types of membrane has also been combined with bioactive ions, including strontium , silver , and zinc (Table ), to enhance their biological performance in vivo.…”

New Resorbable Membrane Materials for Guided Bone Regeneration

“…Several studies have reported changes in mechanical properties and outcomes of GBR by adding additives to collagen membrane (Table 1). The percentage of new bone formation in (iii), (iv), and (v) was higher than (i) and (ii) • No significant difference in bone formation among (iii), (iv), and (v) [13] 5 mm Calvarial defect (rat) (i) Untreated collagen membranes (ii) EGCG cross-linked collagen membranes (iii) Nano-HA modified collagen membranes (iv) Nano-HA modified EGCG-collagen membranes…”

“…Collagen has been used as a resorbable membrane for GBR [11]. Collagen membrane has the advantages of low antigenicity, high biocompatibility, and excellent cell affinity, but pure collagen has the disadvantage of being difficult to handle due to a paucity of physical properties, and it is degraded quickly [12][13][14].…”

“…Three different chitosan concentrations (0.5%, 1%, and 2%) and five different concentrations of β-TCP (0%, 17%, 29%, 38%, and 44%) [29] Collagen/hydroxyapatite (HA) membrane is known to have good biocompatibility, osteoconductivity, and a similar composition and structure to natural bone [25,27]. However, collagen/HA membrane does not have a substantially positive effect on bone regeneration [13,25,26].…”