Guided bone regeneration with subperiosteal implants of PTFE and hydroxyapatite physical barriers in rats

Macedo, Nelson Luiz de; Macedo, Luís Guilherme Scavone de; Matuda, Fábio da Silva; Ouchi, Suzana Martins; Monteiro, Adriana Socorro Ferreira; Carvalho, Yasmin Rodarte

doi:10.1590/s0103-64402003000200009

Cited by 15 publications

(15 citation statements)

References 14 publications

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“…Unfortunately, bone formation with CHA blocks offers a lower capacity for osteogenesis than that provided by particles. 19 At present, autogenous blocks and xenogenic particles are more widely used than xenogenic blocks in the restoration of critical-size alveolar defects. Indeed, there are still few reports of CHA block grafting for alveolar defects.…”

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confidence: 99%

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Du¹,

Liu²,

Deng³

et al. 2015

IJN

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To improve the regenerative performance of nano-hydroxyapatite/coralline (nHA/coral) block grafting in a canine mandibular critical-size defect model, nHA/coral blocks were coated with recombinant human vascular endothelial growth factor 165 (rhVEGF) via physical adsorption (3 μg rhVEGF 165 per nHA/coral block). After the nHA/coral blocks and VEGF/nHA/coral blocks were randomly implanted into the mandibular box-shaped defects in a split-mouth design, the healing process was evaluated by histological observation and histomorphometric and immunohistological analyses. The histological evaluations revealed the ingrowth of newly formed blood vessels and bone at the periphery and cores of the blocks in both groups at both 3 and 8 weeks postsurgery, respectively. In the histomorphometric analysis, the VEGF/nHA/coral group exhibited a larger quantity of new bone formation at 3 and 8 weeks postsurgery. The percentages of newly formed bone within the entire blocks in the VEGF/nHA/coral group were 27.3%±8.1% and 39.3%±12.8% at 3 weeks and 8 weeks, respectively, and these values were slightly greater than those of the nHA/coral group (21.7%±3.0% and 32.6%±10.3%, respectively), but the differences were not significant ( P >0.05). The immunohistological evaluations revealed that the neovascular density in the VEGF/nHA/coral group (146±32.9 vessel/mm 2 ) was much greater than that in the nHA/coral group (105±51.8 vessel/mm 2 ) at the 3-week time point ( P <0.05), but no significant difference was observed at the 8-week time point (341±86.1 and 269±50.7 vessel/mm 2 , respectively, P >0.05). The present study indicated that nHA/coral blocks might be optimal scaffolds for block grafting in critical-size mandibular defects and that additional VEGF coating via physical adsorption can promote angiogenesis in the early stage of bone healing, which suggests that prevascularized nHA/coral blocks have significant potential as a bioactive material for bone regeneration in large-scale alveolar defects.

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mentioning

confidence: 99%

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Du¹,

Liu²,

Deng³

et al. 2015

IJN

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“…A possibilidade de recuperação do tecido perdido em periodontites ou traumas é demonstrada em vários estudos (NOVAES JÚNIOR; MORAES; NOVAES, 1990;GOTTLOW;NYMAN, 1990;PASSERI, 1991;NOVAES JÚNIOR;NOVAES, 1993;LANG;BECKER;KARRING, 1997;CORTELLINI, 1999;CARVALHO et al, 2003;FUGAZZOTTO, 2003a;FUGAZZOTTO, 2003b;LU, 2003;MACEDO et al, 2003). Em 1976, Melcher (1976sugeriu que a cicatrização do periodonto era determinada pelo tipo celular que repopulava a superfície da ferida.…”

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“…Assim, permite-se que células com potencial osteogênico das margens ósseas existentes invadam o espaço criado e produzam osso (NYMAN et al, 1982b;LANG;BECKER;KARRING, 1997;CARVALHO et al, 2003;WIKESJÖ et al, 2003;ASLAN;SIMSEK;DAYL, 2004).…”

Section: Revisãounclassified

“…O selamento periférico entre a barreira e a superfície óssea adjacente à área testada deve prevenir o crescimento de tecido conjuntivo externo e o deslocamento da membrana (ASLAN; SIMSEK; DAYL, 2004). Outro fator de importância é o grau de porosidade da barreira que irá influenciar o resultado da cicatrização (LUNDGREN; LUNDGREN; TAYLOR, 1998), bem como se a membrana é absorvível (LU, 2003) ou não-absorvível (MACEDO et al, 2003), e possui facilidade de aplicação.…”

Section: Revisãounclassified

“…Durante a evolução dos mamíferos, um número reduzido de células pluripotencias se mantém, mas diminuem com o passar do tempo (CARPLAN, 1987). Macedo et al (2003) concluíram que a porosidade da barreira física não foi absolutamente necessária para a neoformação óssea na técnica de RTG; a quantidade de osso formado depende do volume do espaço criado pela membrana.…”

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Uso de membrana biossintética a base de celulose na regeneração tecidual guiada

Iamaguti

Brandão

2007

Sem. Ci. Agr.

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ResumoA Regeneração Tecidual Guiada (RTG) consiste numa modalidade de tratamento regenerativo que requer a colocação de uma barreira física sobre o defeito ósseo, de modo que a proliferação de tecidos moles adjacentes para dentro da área protegida seja evitada. Assim, a regeneração óssea guiada permite que células com potencial osteogênico das margens ósseas existentes invadam o espaço criado e produzam osso. As barreiras físicas devem ser biocompatíveis, permitir a oclusão celular, manutenção do espaço, integração tecidual e facilidade de uso. Podem ser oclusivas ou permeáveis, absorvíveis ou não-absorvíveis. Dentre os vários tipos de barreiras físicas existentes no mercado, a membrana biossintética a base de celulose vem se destacando, pois, além de possuir as características necessárias para a RTG, trata-se de um produto nacional desenvolvido a baixos custos. Palavras-chave: Regeneração tecidual guiada, cartilagem articular, membrana de celulose AbstractGuided Tissue Regeneration (GTR) is a regenerative treatment modality that requires the placement of a physical barrier over a bone defect in such a way that the proliferation of the surrounding soft tissues into the barrier-protected area is prevented. Thereby in the guided osseous regeneration allowing boneforming cells from the existent bone edges to invade the space and produce bone. The physical barriers should be biocompatible, allow cellular occlusion, maintain adequate space, tissue integration and facility in the application. They can be occlusive or permeable, absorbable or non-absorbable. Among various types of physical barriers in the market, the cellulose biosynthetic membrane is emphasizing by the necessary characteristics for GTR and to be a national product developed in low cost.

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Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration

Borges

Barros

Garms

et al. 2017

J of Applied Polymer Sci

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Natural rubber latex (NRL) from Hevea brasiliensis is a colloidal system composed of cis-1,4-polyisoprene. Its applications have grown due its angiogenic and wound healing activity. NRL has been used in guided bone regeneration as barrier, enhancing bone formation. However, there has been no study reported so far which shows its in vitro biocompatibility with osteoblasts. Thus, the aim of this work was to apply thermally induced phase separation under several temperatures to induce porosity in NRL; and test its mechanical properties, cytotoxicity, cell adhesion, and mineralization with MC3T3-E1. Only biomembranes submitted at 220 and 210 8C presented porosity. Fourier transform infrared spectroscopy showed no change in cis-1,4-isoprene spectra. Biomembranes were elastic (Young's modulus < 1 MPa). According to ISO10993-5, NRL showed no cytotoxicity. Cells adhered on the NRL and produced mineral matrix as analyzed by scanning electron microscopy-energy-dispersive spectrometry, von kossa, and Fourier transform infrared spectroscopy. Cells on NRL presented higher alkaline phosphatase activity, however, mineralization showed no difference by alizarin red S dye extraction.

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Guided bone regeneration with subperiosteal implants of PTFE and hydroxyapatite physical barriers in rats

Cited by 15 publications

References 14 publications

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Uso de membrana biossintética a base de celulose na regeneração tecidual guiada

Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration

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