Guidelines for a Morphometric Analysis of Prokaryotic and Eukaryotic Cells by Scanning Electron Microscopy

Czerwińska-Główka, Dominika; Krukiewicz, Katarzyna

doi:10.3390/cells10123304

Cited by 17 publications

(5 citation statements)

References 93 publications

(140 reference statements)

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“…Sequentially, dehydration was performed once each using 30, 50, 70, 85, and 95% ethanol, and twice using 100% ethanol. Each dehydration time was 15 ~ 20 min ( Czerwińska-Główka and Krukiewicz, 2021 ). The sample was replaced with isoamyl acetate two times, each time for 20 min, and then centrifuged to obtain the precipitate, which was made into powder by vacuum freeze-drying.…”

Section: Methodsmentioning

confidence: 99%

Recovery of terbium by Lysinibacillus sp. DW018 isolated from ionic rare earth tailings based on microbial induced calcium carbonate precipitation

Bian,

Dong,

Ning

et al. 2024

Front. Microbiol.

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Microbial induced calcium carbonate precipitation (MICP) is considered as an environmentally friendly microbial-based technique to remove heavy metals. However, its application in removal and recovery of rare earth from wastewaters remains limited and the process is still less understood. In this study, a urease-producing bacterial strain DW018 was isolated from the ionic rare earth tailings and identified as Lysinibacillus based on 16S rRNA gene sequencing. Its ability and possible mechanism to recover terbium was investigated by using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and fourier transform infrared spectroscopy (FTIR). The results showed that the urease activity of DW018 could meet the biomineralization requirements for the recovery of Tb3+ from wastewaters. The recovery rate was as high as 98.28% after 10 min of treatment. The optimal conditions for mineralization and recovery were determined as a bacterial concentration of OD600 = 1.0, a temperature range of 35 to 40°C, and a urea concentration of 0.5%. Notably, irrespective of CaCO3 precipitation, the strain DW018 was able to utilize MICP to promote the attachment of Tb3+ to its cell surface. Initially, Tb3+ existed in amorphous form on the bacterial surface; however, upon the addition of a calcium source, Tb3+ was encapsulated in calcite with the growth of CaCO3 at the late stage of the MICP. The recovery effect of the strain DW018 was related to the amino, hydroxyl, carboxyl, and phosphate groups on the cell surface. Overall, the MICP system is promising for the green and efficient recovery of rare earth ions from wastewaters.

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Section: Methodsmentioning

confidence: 99%

Recovery of terbium by Lysinibacillus sp. DW018 isolated from ionic rare earth tailings based on microbial induced calcium carbonate precipitation

Bian,

Dong,

Ning

et al. 2024

Front. Microbiol.

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“…H. pylori morphology was observed using SEM following methods described in the previous literature 53 . Briefly, after coculture with the nanoparticles, the mixture was centrifuged at 5000 rpm (SCILOGEX, CF1524R) for 5 min, after which the supernatant was removed.…”

Section: Methodsmentioning

confidence: 99%

An antibiotic-free platform for eliminating persistent Helicobacter pylori infection without disrupting gut microbiota

Lai,

Zhang,

Yin

et al. 2024

Acta Pharmaceutica Sinica B

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“…The morphology of the granules in contact with MLO‐Y4 cells was also investigated by scanning electron microscopy (SEM) using a FEI Quanta 200 instrument (Fei Company, Eindhoven, The Netherlands), coupled to an INCA 350 EDS apparatus (Oxford Instruments, Abingdon, United Kingdom). After 24 h of incubation with the granules, the cells were rinsed with Dulbecco's phosphate buffer solution (DPBS), fixed in glutaraldehyde 2.5% (G5882‐Sigma‐Merck, Darmstadt, Germany), dehydrated with ascending ethanol scale, sputtered by 10 nm layer of gold and observed 35 …”

Section: Methodsmentioning

confidence: 99%

In‐vivo evaluations of bone regenerative potential of two novel bioactive glasses

Anesi

Ferretti

Salvatori

et al. 2023

J Biomedical Materials Res

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Due to the aging of population, materials able to repair damaged tissues are needed. Among others, bioactive glasses (BGs) have attracted a lot of interest due to their outstanding properties both for hard and soft tissues. Here, for the first time, two new BGs, which gave very promising results in preliminary in vitro‐tests, were implanted in animals in order to evaluate their regenerative potential. The new BGs, named BGMS10 and Bio_MS and containing specific therapeutic ions, were produced in granules and implanted in rabbits' femurs for up to 60 days, to test their biocompatibility and osteoconduction. Additionally, granules of 45S5 Bioglass® were employed and used as a standard reference for comparison. The results showed that, after 30 days, the two novel BGs and 45S5 displayed a similar behavior, in terms of bone amount, thickness of new bone trabeculae and affinity index. On the contrary, after 60 days, 45S5 granules were mainly surrounded by wide and scattered bone trabeculae, separated by large amounts of soft tissue, while in BGMS10 and Bio_MS the trabeculae were thin and uniformly distributed around the BG granules. This latter scenario could be considered as more advantageous, since the features of the two novel BG granules allowed for the neo‐formation of a uniformly distributed bony trabeculae, predictive of more favorable mechanical behavior, compared to the less uniform coarse trabeculae, separated by large areas of soft tissue in 45S5 granules. Thus, BGMS10 and Bio_MS could be considered suitable products for tissue regeneration in the orthopedic and dental fields.

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Guidelines for a Morphometric Analysis of Prokaryotic and Eukaryotic Cells by Scanning Electron Microscopy

Cited by 17 publications

References 93 publications

Recovery of terbium by Lysinibacillus sp. DW018 isolated from ionic rare earth tailings based on microbial induced calcium carbonate precipitation

Recovery of terbium by Lysinibacillus sp. DW018 isolated from ionic rare earth tailings based on microbial induced calcium carbonate precipitation

An antibiotic-free platform for eliminating persistent Helicobacter pylori infection without disrupting gut microbiota

In‐vivo evaluations of bone regenerative potential of two novel bioactive glasses

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