Guidelines for the Use and Care of Small Laboratory Animals in Transplantation Research

Geise, W.

doi:10.1007/978-3-642-72140-3_4

Cited by 2 publications

(2 citation statements)

References 17 publications

(12 reference statements)

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“…The operative approach to the femoral artery, a peripheral vessel with a relatively high percentage of muscle cells in its wall, avoid laparotomy and exposition of vital structures to direct radiation and systemic alterations (there is no radiosensitive organ in the extremities), lesser hydroelectrolitic disturbs besides this region often has been used in vascular surgery practice (angioplasty, bypass) 24 .…”

Section: Discussionmentioning

confidence: 99%

Effects of external gamma radiation on femoral artery reimplantation in rats: morphometrical analyzes

et al. 2003

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Costa RFB, Fagundes DJ, Juliano Y, Novo NF, Vieira WTT. Effects of external gamma radiation on femoral artery reimplantation in rats: morphometrical analyzes. Acta Cir Bras [serial online] 2003 Mar-Apr;18(2). Available from URL: http://www.scielo.br/acb. ABSTRACT -Purpose: To investigate the effects of external gamma radiation on rat femoral artery reimplant. Methods: Sixty-two male Wistar rats were distributed in two groups I (Control) and II (Irradiated), both formed by three observation subgroups: 2 (10 animals), 7 (11) and 21(10) postoperative days (PO). The right femoral artery of each animal was split up and reimplanted (end-to-end anastomosis), through microsurgery technique. In the first PO day group II animals were irradiated with a single dose of 15Gy, external source. The histological analysis, qualitative and descriptive analysis, was accomplished through hematoxylin and eosin (HE), Verhoeff and Masson trichromium methodology. Morphometric analysis was realized in the same slides used to histological analysis stained by HE. Computer software was used to quantify morphological alterations of the vascular wall, by processing captured images from a microscope. It was analyzed the intimal layer (intimal hyperplasia and endothelium cells formation) and the nuclei percentage of smooth muscular cell in the medium layer and in the intima hyperplasia. The data were treated applying the statistical tests: Fisher s exact, Chi-square, Mann-Whitney, Kruskal-Wallis and analysis of variance (p < .05). Results: The patency graft was observed by microscope distal to the autograft in 93.5% (29/31). It was observed that the gamma-radiation reduced the degree of covering of the vascular wall for the neo-formed endothelium. The nuclei percentage of smooth muscular cells, in the media, was smaller compared to control animals, although it was no significant for the studied sample size. The irradiated group showed a significant lower luminal stenosis and the intimal hyperplasia occurrence. In this group the percentage of smooth muscular cells proliferation, in the media, were significantly lower for 7 and 21 days of observation. Conclusion: The external single dose of 15 Gy gamma-radiation delivered at first postoperative day may inhibit the endothelial cells regeneration and the percentage of lumen stenosis and prevents intimal hyperplasia, in the 2, 7 and 21 PO on femoral artery autograft in rats.

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Section: Discussionmentioning

confidence: 99%

Effects of external gamma radiation on femoral artery reimplantation in rats: morphometrical analyzes

et al. 2003

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“…As a result of this, the conducting scientist has a major responsibility toward science and animal welfare. Furthermore, one has to focus on the legal, ethical, and social impact [1].…”

Section: Introductionmentioning

confidence: 99%

Animal Models in Allogenic Solid Organ Transplantation

Wenzel¹,

Blasczyk²,

Figueiredo³

2021

Transplantology

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Animal models provide the link between in vitro research and the first in-man application during clinical trials. They provide substantial information in preclinical studies for the assessment of new therapeutic interventions in advance of human clinical trials. However, each model has its advantages and limitations in the ability to imitate specific pathomechanisms. Therefore, the selection of an animal model for the evaluation of a specific research question or evaluation of a novel therapeutic strategy requires a precise analysis. Transplantation research is a discipline that largely benefits from the use of animal models with mouse and pig models being the most frequently used models in organ transplantation research. A suitable animal model should reflect best the situation in humans, and the researcher should be aware of the similarities as well as the limitations of the chosen model. Small animal models with rats and mice are contributing to the majority of animal experiments with the obvious advantages of these models being easy handling, low costs, and high reproductive rates. However, unfortunately, they often do not translate to clinical use. Large animal models, especially in transplantation medicine, are an important element for establishing preclinical models that do often translate to the clinic. Nevertheless, they can be costly, present increased regulatory requirements, and often are of high ethical concern. Therefore, it is crucial to select the right animal model from which extrapolations and valid conclusions can be obtained and translated into the human situation. This review provides an overview in the models frequently used in organ transplantation research.

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Guidelines for the Use and Care of Small Laboratory Animals in Transplantation Research

Cited by 2 publications

References 17 publications

Effects of external gamma radiation on femoral artery reimplantation in rats: morphometrical analyzes

Effects of external gamma radiation on femoral artery reimplantation in rats: morphometrical analyzes

Animal Models in Allogenic Solid Organ Transplantation

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