H-2 compatibility is required for T-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus.

Abstract

Maximal cell-mediated lysis of targets infected with lymphocytic choriomeningitis virus occurs only within a H-2 compatible system. Syngeneic immune spleen cells are at least 100 times as effective as are allogeneic lymphocytes. Reciprocal restriction of cytotoxic T-cell activity has been shown to operative between H-2k, H-2d, and H-2b. Experiments with cogenic mice have localized the effect to the H-2 gene complex. Furthermore, the observation that lymphocytes from H-2a mice cause high specific 51Cr release from either H-2d virus-infected cells, indicates that identity at either the K or the D end of the H-2 gene complex is sufficient for this lytic interaction.

Specificity studies on cytotoxic thymus-derived lymphocytes reactive with influenza virus-infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens.

“…This interest stems largely from the observation that cytotoxic thymus-derived (T) lymphocytes generated in mice in response to viral infection will lyse virus-infected H-2-compatible, but not H-2 incompatible, target cells in the 5lCr release cytotoxicity assay. This observation, initially reported with lymphocytic choriomeningitis virus (1), has been confirmed with vaccinia (2), ectromelia (3), Sendai (4), and 1; riend leukemia (5) viruses. Two general explanations have been proposed to account for the requirement of H-2 compatibility between lymphocytes and virus-infected target cells.…”

“…The alternative concept is that T cells recognize either normal or modified H-2 antigens and that both viral and H-2 specific antigenic recognition are required for cell lysis. This concept has been referred to as the "physiological interaction" or "dual recognition" hypothesis (1,6,9). Published studies have indicated that lymphocytic choriomeningitis, ectromelia, and Sendai virusinfected target cells can be distinguished by cytotoxic T lymphocytes from appropriately immunized syngeneic mice (4).…”

Bifunctional major histocompatibility-linked genetic regulation of cell-mediated lympholysis to trinitrophenyl-modified autologous lymphocytes.

“…Since the recent independent discoveries that viruses (8)(9)(10)(11)(12), and chemicals Tables I and II, however, semiallogeneic or allogeneic CML reactions with unmodified stimulating cells generated effectors which lysed to a variable extent (8 .1-23 .1%) any TNP-modified target spleen cell . This observation illustrates the importance of using only syngeneic mixtures of responder and stimulating cells in the sensitization phase of the CML .…”

H-2 compatibility requirement for virus-specific T-cell-mediated cytolysis. Evaluation of the role of H-2I region and non-H-2 genes in regulating immune response.

“…Macrophages are members of the reticuloendothelial system and are probably the first cells to take up free virus from the circulation. Although it is not known as yet how and if cytotoxic T cells (24)(25)(26)(27)(28) play any direct role in controlling virus growth and/or spreading, interaction (cytolytic or noncytolytic in vivo) between T cells and infected macrophages are probably important for this function (reviewed in 29). These cells may thus represent biologically relevant targets.…”