H. G. Wells, a Biography

Exo-site affinity labeling of C1.¯ a subcomponent of the first component of complement, by m-[o-(2-chloro-5-fluorosulfonylphenylureido)phenoxybutoxy]benzamidine

“…The inactivation was performed as described in Materials and Methods. The pseudo-first-order rate constants kl = 6.3 X 10-7 s-1 for the methanol control 1.35 X 10-5 s_i for 222 nM mCP(MB)-F and 1.5 X 10~4 for 233 mM mCP(PBA)-F. ison, in the Cls nitroanilide substrate Boc-Phe-Val-Arg-Na (Morgan & Nair, 1977), the scissile bond is 0.65 nm from the cationic guanidinium group and thus approximates the size of catalytic site of Cls. The high binding affinity of wtCP(PBA)-OH, compared with benzamidine, implies that mCP(PBA)-F binds not only via its specific ligand but also via interactions of the extended side chain and that this chain extension has restricted orientations possibly within the peptide binding groove (Feldmann et al, 1978).…”

“…Cls hydrolyzes a number of synthetic substrates. The esters A-Ac-Gly-L-Lys-OMe, A-Ac-L-Lys-OMe, A-Ts-L-Arg-OMe, A'-Ac-Tyr-OEt, A-Z-Lys-ONp, and A-Z-L-Tyr-ONp and a tripeptide anilide Boc-Phe-Val-Arg-Na are hydrolyzed (Bing, 1969;Cooper & Ziccardi, 1976;Harpel, 1970; Nagaki & Stroud, 1969; Morgan & Nair, 1977). The turnover rate of these substrates by Cls is very slow compared with the activity of other enzymes such as trypsin, thrombin, and plasmin toward the same compounds (Cooper & Ziccardi, 1976;Morgan & Nair, 1977, Andrews et al, 1978.…”

“…The esters A-Ac-Gly-L-Lys-OMe, A-Ac-L-Lys-OMe, A-Ts-L-Arg-OMe, A'-Ac-Tyr-OEt, A-Z-Lys-ONp, and A-Z-L-Tyr-ONp and a tripeptide anilide Boc-Phe-Val-Arg-Na are hydrolyzed (Bing, 1969;Cooper & Ziccardi, 1976;Harpel, 1970; Nagaki & Stroud, 1969; Morgan & Nair, 1977). The turnover rate of these substrates by Cls is very slow compared with the activity of other enzymes such as trypsin, thrombin, and plasmin toward the same compounds (Cooper & Ziccardi, 1976;Morgan & Nair, 1977, Andrews et al, 1978. Like these enzymes, however, Cls is competitively inhibited by substituted benzamidines and phenylguanidine as well as a variety of N-a-blocked tyrosines (Bing, 1969;Andrews et al, 1978), further illustrating the hydrophobic and ionic nature of the implicating the importance of the Cls esteratic site (Stroud et al, 1965).…”

Beyond Morgan’s eight metaphors: Adding to and developing organization theory

“…It was a ground-breaking book in many respects, not the least because it was an unusual and thought-provoking text on organization theory. The book has been cited 15,496 times (Google Scholar, 2015) and translated into 14 languages (Morgan, 2015). Its first edition sold over 250,000 copies (Oswick and Grant, 2015), and the 1996a and 2006 versions sold more than 100,000 copies (Maggie Stanley, SAGE, August 2015, personal communication) [all citation and sales figures are for the English version only].…”