H19 and MEST gene expression and histone modification in blastocysts cultured from vitrified and fresh two-cell mouse embryos

Jahangiri, Maryam; Shahhoseini, Maryam; Movaghar, Bahar

doi:10.1016/j.rbmo.2014.07.006

Cited by 16 publications

(10 citation statements)

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“…Thus, the preimplantation embryo is susceptible to epigenetic modifications (Santos & Dean, 2004). Recently, an increasing amount of material has been published that pertains to the effect of vitrification on the methylation status of the differentially methylated domain, expressions of a number of imprinted genes, and global DNA methylation (Bakhtari et al, 2014;Jahangiri, Shahhoseini, & Movaghar, 2014). Most often, human embryos undergo vitrification on Day 3 (6-8 cell stage) in ART.…”

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confidence: 99%

Aberrant expression of miR‐29a/29b and methylation level of mouse embryos after in vitro fertilization and vitrification at two‐cell stage

Movahed

Soleimani

Hosseini

et al. 2019

Journal Cellular Physiology

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Proper epigenetic modifications during preimplantation embryo development are important for a successful pregnancy. We aim to investigate the putative influence of in vitro fertilization (IVF) and vitrification on DNA methylation in mouse preimplantation embryos. The study groups consisted of blastocyst‐derived vitrified two‐cell embryos, nonvitrified embryos, and a control group of in vivo derived blastocysts. We assessed developmental competence, global DNA methylation, relative expression levels of miR‐29a/29b, and their target genes, Dnmt3a/3b. Vitrified embryos had a lower developmental rate as compared with nonvitrified embryos. There was no significant decrease in blastocyst cell numbers among studied groups, whereas there was a steady decline in DNA methylation after IVF and vitrification. The levels of miR‐29a/29b upregulated in the experimental groups as compared with the control group. IVF and vitrification caused Dnmt3a/3b downregulations in blastocysts. The results of this study have suggested that a relationship exists between IVF and embryo vitrification with methylation interruptions in the blastocysts.

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mentioning

confidence: 99%

Aberrant expression of miR‐29a/29b and methylation level of mouse embryos after in vitro fertilization and vitrification at two‐cell stage

Movahed

Soleimani

Hosseini

et al. 2019

Journal Cellular Physiology

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“…Other studies indicated that IVF and Whitten's and KSOM media down-regulated H19 expression (35). However, Jahangiri et al reported that vitrification of two-cell stage embryos had no effect on H19 and MEST expression patterns (15). Nonetheless, most recent studies have demonstrated that some types of manipulation and embryo media may alter imprinting gene expression through DNA methylation and histone modifications (36).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, about 100 proteins encoded by imprinted genes have been identified in both mice and human genomes (14). In mice embryos, H19/Igf2 imprinting genes have greater sensitivity to culture supplements and micromanipulations than other imprinted genes (15,16). Also, H19/Igf2 have an essential function in the control of embryo development, placental organization, and fetal growth (17).…”

Section: Introductionmentioning

confidence: 99%

“…Differential epigenetic modification at the ICR and upstream of the transcription site of the imprinting genes in the preimplantation embryo have been observed (21). For instance, lysine methylations restricted to the promoter of the imprinted loci are H3 lysine 9 trimethylation (H3K9me3) and H3 lysine 4 trimethylation (H3K4me3), which lead to inhibition and activation, respectively, of gene expression in imprinting genes (15). Recent studies have shown that embryo culture and manipulations, including cryopreservation, intracytoplasmic sperm injection (ICSI), and somatic cell nuclear transfer (SCNT) can lead to the abnormal histone methylation in promoter regions of the imprinting genes (22,23).…”

Section: Introductionmentioning

confidence: 99%

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H19/Igf2 Expression and Methylation of Histone 3 in Mice Chimeric Blastocysts

et al. 2020

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Background: Currently, the efficient production of chimeric mice and their survival are still challenging. Recent researches have indicated that preimplantation embryo culture media and manipulation lead to abnormal methylation of histone in the H19/Igf2 promotor region and consequently alter their gene expression pattern. This investigation was designed to evaluate the relationship between the methylation state of histone H3 and H19/Igf2 expression in mice chimeric blastocysts. Methods: Mouse 129/Sv embryonic stem cells (mESCs) expressing the green fluorescent protein (mESCs-GFP) were injected into the perivitelline space of 2.5 days post-coitis (dpc) embryos (C57BL/6) using a micromanipulator. H3K4 and H3K9 methylation, and H19 and Igf2 expression was measured by immunocytochemistry and q-PCR, respectively, in blastocysts. Results: Histone H3 trimethylation in H3K4 and H3K9 in chimeric blastocysts was significantly less and greater, respectively (p< 0.05), than in controls. H19 expression was significantly less (p< 0.05), while Igf2 expression was less, but not significantly so, in chimeric than in control blastocysts. Conclusions: Our results showed, that the alteration ofH3K4me3 and H3K9me3 methylation, change H19/Igf2 expression in chimeric blastocysts.

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“…Mest expression in the mouse coincides with placental and decidual angiogenesis and is localized to the developing capillary endothelium (Mayer et al 2000). Some studies have shown that assisted reproductive technology, such as IVF, influences DNA methylation and expression of MEST in humans and mice (Jahangiri et al 2014). Embryo culture conditions have been shown to affect DNA methylation and imprinted gene expression in several animal models (Nelissen et al 2012).…”

Section: Discussionmentioning

confidence: 99%

A novel embryo culture media supplement that improves pregnancy rates in mice

et al. 2017

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The preimplantation embryo in vivo is exposed to numerous growth factors in the female reproductive tract, which are not recapitulated in embryo culture media in vitro The IGF2 and plasminogen activator systems facilitate blastocyst development. We hypothesized that the addition of IGF2 in combination with urokinase plasminogen activator (uPA) and plasminogen could improve rates of blastocyst hatching and implantation in mice. B6BcF1 and CBAB6F2 mouse embryos were divided into one of four supplemented culture media treatment groups: (1) control (media only); (2) 12.5 nM IGF2; (3) 10 µg/mL uPA and 5 µg/mL plasminogen; or (4) a combination of IGF2, uPA and plasminogen treatments. Embryo development to blastocyst stage and hatching were assessed before transfer to pseudopregnant recipient females and implantation, pregnancy rates and postnatal growth were assessed. After 90.5 h of culture, IGF2 + U + P treatment increased the percentage of B6BcF1 embryos that were hatching/hatched and percentage developing to blastocyst stage compared with controls (P < 0.02). Following B6BcF1 embryo transfer, IGF2 + U + P treatment increased implantation sites at day 8 of pregnancy compared with controls (P < 0.05). Replication in the CBAB6F2 mouse strain showed significant improvements in pregnancy rates at days 8 and 18 but not in blastocyst development. No adverse effects were seen on gestational age, litter size or birthweight, or the reproductive capacity of offspring of IGF2 + U + P treated embryos. For embryos susceptible to detrimental effects of in vitro culture, IGF2, uPA and plasminogen supplementation of culture media can improve pregnancy success, but the effect of treatment is dependent on the mouse strain.

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H19 and MEST gene expression and histone modification in blastocysts cultured from vitrified and fresh two-cell mouse embryos

Cited by 16 publications

References 44 publications

Aberrant expression of miR‐29a/29b and methylation level of mouse embryos after in vitro fertilization and vitrification at two‐cell stage

Aberrant expression of miR‐29a/29b and methylation level of mouse embryos after in vitro fertilization and vitrification at two‐cell stage

H19/Igf2 Expression and Methylation of Histone 3 in Mice Chimeric Blastocysts

A novel embryo culture media supplement that improves pregnancy rates in mice

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