Habitual Physical Activity is Associated with Relative Apelin Gene Expression in Adipose Tissues Among Non-Diabetic Adults

Mahmoodi, Behnaz; Shemshaki, Afsaneh; Zarkesh, Maryam; Hedayati, Mehdi; Mirmiran, Parvin

doi:10.1007/s10989-018-09800-4

Cited by 5 publications

(7 citation statements)

References 25 publications

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“…Leisure-time PA refers to exercise, sports, or recreation, which is characterized by high, moderate, or intensive activity that is not related to regular work, housework, or transport activities directly associated with PPARγ expression. The current recommendations mostly concentrated on the health benefit of Leisure-time PA on preventing and treating metabolic disorders; however, occupation, transportation, and housework may affect adipose tissue metabolism ( 16 ). Housework-related activity is an important factor that could have contributed to enhancing the total activity level, which female adults primarily carry out.…”

Section: Discussionmentioning

confidence: 99%

“…Habitual PA was calculated from sum of leisure time, occupation, household work, and transportation-related activity in MET-minutes per week. Participants were categorized as either sedentary(PA<600MET-minutes per week) or active(≥600MET-min per week)( 16 , 20 ). Dietary intake was collected using a valid and reliable semi-quantitative food frequency questionnaire (FFQ) ( 21 ).…”

Section: Methodsmentioning

confidence: 99%

“…Studies that differ in training intensity and duration report variable findings on exercise-induced gene expression of PPARγ in adipose tissues ( 14 , 15 ). However, people who did not have usual PA had a chronic impact on adipose tissue gene regulation ( 16 ). It remains unknown whether PPARγ expression in adipose tissues changes with habitual PA in humans with a wide range of obesity.…”

Section: Introductionmentioning

confidence: 99%

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Physical Activity and Exercise Promote Peroxisome Proliferator-Activated Receptor Gamma Expression in Adipose Tissues of Obese Adults

Zarkesh¹,

Nozhat²,

Akbarzadeh³

et al. 2022

ijph

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Background: Peroxisome proliferator-activated receptor gamma (PPARγ) has recently been studied for its potential influence on the functional response of the human body to exercise. We aimed to investigate the association of habitual physical activity (PA) with PPARγ mRNA level in the visceral and subcutaneous adipose tissues (VAT and SAT) in non-obese and obese non-diabetic adults. Methods: VAT and SAT were obtained from 95 individuals, including 40 non-obese (BMI<30kg/m2) and 55 obese (BMI≥30kg/m2) who underwent elective abdominal surgery (Tehran, Iran, 2012-2015). The assessment of habitual PA was performed by a valid and reliable International PA Questionnaire-long form, and the metabolic equivalent of task (MET) was evaluated. Real-time quantitative reverse transcriptase-PCR evaluated the PPARγ expression in VAT and SAT. Results: PPARγ expression in both VAT (1.18 vs. 0.37 fold change, P<0.001) and SAT (2.07 vs. 0.29 fold change, P=0.004) among obese subjects was higher than the non-obese group. After controlling for age, sex, and total energy intake, a positive association was found between total METs and PPARγ expression in both VAT and SAT among obese participants (β=0.22, P=0.007 and β=0.12, P<0.001, respectively). Among obese participants, there was a direct association between leisure time-related METs with VAT PPARγ expression (β=0.05, P=0.026). Moreover, in this group, an association was observed between occupation-related METs with PPARγ in both fat tissues (β=0.11, P=0.002 and β=0.17, P=0.013, respectively), and household work-related METs with SAT PPARγ (β=0.21, P=0.011). Conclusion: High PA as an indispensable part of a healthy lifestyle may exert its beneficial effect by regulating PPARγ expression.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physical Activity and Exercise Promote Peroxisome Proliferator-Activated Receptor Gamma Expression in Adipose Tissues of Obese Adults

Zarkesh¹,

Nozhat²,

Akbarzadeh³

et al. 2022

ijph

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“…Physical activity was assessed using a validated and reliable Persian version of the International Physical Activity Questionnaire Long-Form (IPAQ-LF); the metabolic equivalent minutes (MET) per week was also calculated [ 18 , 19 ]. Dietary intakes were collected through face-to-face interviews by a trained dietitian, using a valid and reliable semi-quantitative food frequency questionnaire (FFQ) [ 20 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

Association of miR-34a and miR-143 levels with PPARγ gene expression in adipose tissues of non-diabetic adults

Zarkesh

Tabaei

Akbarzadeh

et al. 2022

J Physiol Anthropol

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Background Peroxisome proliferator-activated receptor gamma (PPARγ) is a promising therapeutic molecule. Epigenetic mechanisms, including non-coding RNAs, regulate the expression level of the PPARγ gene. Objective We aimed to examine the PPARγ expression in non-diabetic individuals in four body mass index (BMI) categories and its association with miR-34a and miR-143 expression. Methods Visceral and subcutaneous adipose tissues (VAT and SAT) samples were collected from patients undergoing bariatric or elective open abdominal surgeries. The subjects (mean age: 42±14.8 years) included 18 normal-weight, 19 overweight, 18 obese, and 19 morbidly obese individuals. The RNAs levels were determined by quantitative real-time PCR. Results The PPARγ expression was significantly upregulated in both adipose depots of the morbidly obese subjects compared to the normal group. SAT PPARγ level was significantly increased in the obese group compared to the normal-weight group (P<0.01); this increase was also significant in the SAT of morbidly obese subjects compared to the overweight cases (P=0.02). Differences in the regulation of PPARγ expression in both SAT and VAT were significant between the four groups (P<0.05). While miR-143 was overexpressed in the SAT of obese and morbidly obese individuals compared to the normal-weight group, the pairwise comparison showed no significant difference in the miR-34a expression of SAT between the four BMI groups (P>0.01). After controlling for the confounding factors, the expression of VAT PPARγ was directly associated with the miR-34a level in the normal-weight group (β=0.311, P=0.010). A negative association was observed between the VAT PPARγ expression and miR-34a expression in obese cases (β = − 0.594, P=0.039). Conclusion The results also confirmed the regulatory function of microRNAs in the PPARγ expression and adipogenesis.

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“…The PCR was completed using the following thermal programs: initial denaturation (5 min at 95°C) and then a three-step amplification program (10 s at 95°C followed by 25 s at 62°C and 35 s at 72°C), with the melting curve, repeated 40 times. GAPDH was used as the reference gene to normalize mRNA levels, based on previously published studies [20]. All experiments were repeated twice.…”

Section: Methodsmentioning

confidence: 99%

Is Habitual Dietary Intake of Fats Associated with Apelin Gene Expression in Visceral and Subcutaneous Adipose Tissues and Its Serum Levels in Obese Adults?

Zarkesh

Safarian

Asghari

et al. 2022

Public Health Genomics

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Introduction: Apelin could be one of the last protective defenses before developing obesity-related disorders, including insulin resistance, type 2 diabetes, and hypertension, which can be modified by dietary intake. The present study investigated the association of habitual intake of total fatty acids (TFAs), saturated-, monounsaturated-, polyunsaturated FAs, n-3, and n-6 FAs with Apelin expression in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT). Methods: We obtained VAT and SAT from 168 participants (64 nonobese and 104 obese) who had undergone open abdominal surgery. Dietary intake information was gathered with a valid and reliable food frequency questionnaire. The mRNA expression of the Apelin gene was analyzed by real-time PCR. Results: Apelin serum levels were increased in the obese subjects compared to the nonobese group (p = 0.016). The SAT and VAT Apelin mRNA levels were significantly elevated in the obese participants compared to the nonobese ones (p < 0.05). Based on BMI status, only obese subjects indicated a positive association between SAT and VAT Apelin expression and TFA intake (p < 0.001). However, this association was observed between SAT and VAT Apelin gene expression and polyunsaturated fatty acid (PUFA) and n-3 FA intakes in both obese and nonobese groups (p < 0.05). Conclusion: High Apelin gene expression was associated with TFA intake in obese subjects in both fat tissues. However, habitual intake of PUFA and n-3 FA was associated with Apelin gene expression in obese and nonobese individuals. Our results indicate a determinative role of the quality and quantity of FA intake on adipose tissue.

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Habitual Physical Activity is Associated with Relative Apelin Gene Expression in Adipose Tissues Among Non-Diabetic Adults

Cited by 5 publications

References 25 publications

Physical Activity and Exercise Promote Peroxisome Proliferator-Activated Receptor Gamma Expression in Adipose Tissues of Obese Adults

Physical Activity and Exercise Promote Peroxisome Proliferator-Activated Receptor Gamma Expression in Adipose Tissues of Obese Adults

Association of miR-34a and miR-143 levels with PPARγ gene expression in adipose tissues of non-diabetic adults

Is Habitual Dietary Intake of Fats Associated with Apelin Gene Expression in Visceral and Subcutaneous Adipose Tissues and Its Serum Levels in Obese Adults?

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