HAEMAGLUTINATION OF Shigella flexneri SUBUNIT PILI PROTEIN 18 KDA AS A MOLECULE ADHESION IN MICE ENTEROCYTES

Fitrianingsih, Avin Ainur

doi:10.18860/jip.v2i1.4248

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…These cytokines have also been identified as the other cytokines inhibitor since it inhibits the myeloid cells' ability such as macrophages and dendritic cells to activate Th1 cells so that the cytokines production by Th1 cells is blocked. 12 Another possible cause of the decrease in IL-10 levels is the microorganisms burden, in this study was formed as pili protein along with adjuvant induction, was not high enough to increase the production of IL-10. As other studies explained that infections caused gramnegative bacteria, the microorganism burden can affect the IL-10 response.…”

Section: Discussionmentioning

confidence: 76%

Pili Protein 65.5 kDa of Klebsiella pneumoniae Induced a Decrease in IL-10 in Mice

Agustina

Wati

Wisudanti

et al. 2022

mkb

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Klebsiella pneumoniae is one of the leading cause of nosocomial infection worldwide with clinical isolates mostly found in Multi-Drug Resistant (MDR) or Extensively Drug-Resistant (XDR). This makes therapeutic options due to this bacteria limited. Several studies have shown that the pili protein of K. pneumoniae with different molecular weights plays a role in the virulence factor, as they are hemagglutinin and adhesin proteins. Pili protein 65.5 kDa of K. pneumoniae can be considered as the antigen candidate for vaccines. This study aimed to determine the immune response based on the IL-10 level as induced by pili protein 65.5 kDa K. pneumoniae. This study was conducted from October 2020 to March 2021 at the Faculty of Mathematics and Natural Sciences and Faculty of Medicine of Jember University. This was true experimental with post-test only controlled group design on 27 male BALB/c mice which divided into 3 groups: K1, K2, and K3. The groups were induced with sterile PBS, pili protein and Freund’s adjuvant, and Freund’s adjuvant only, respectively. Samples were drawn through cardiac puncture, then serum was taken to measure the IL-10 levels using the ELISA method. Hemagglutination test showed a positive result with 1/8 as the highest titer. Results showed that the mean IL-10 levels were 290.92±45.33; 235.05±44.53; 218.54±64,81 for K1, K2, and K3, respectively. One-way ANOVA test results showed a statistical difference between groups (p=0,019). This research shows that pili protein 65.5 kDa K. pneumoniae decreases the IL-10 level. However, when it is provided with the Freund’s Adjuvant, there is no proof of the immunogenicity when compared to the control group.

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Section: Discussionmentioning

confidence: 76%

Pili Protein 65.5 kDa of Klebsiella pneumoniae Induced a Decrease in IL-10 in Mice

Agustina

Wati

Wisudanti

et al. 2022

mkb

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“…Based on research in 2017, the results showed that the S. flexneri pili protein subunit was able to cross-react with Omp S. flexneri with Molecular Weight (MW) 23 and 27 kDa, and could cross-react with the pili 18 kDa subunit; 23; 34; and 53 kDa S. flexneri [9]. Then from another study in the same year, it was also found that the 18 kDa S. flexneri pili subunit protein is an adhesive protein that can help attach the pili bacteria to the enterocyte cells of mice [10]. A 2018 study proved that β defensin in the serum, intestinal mucosa, and lungs of mice was increased by oral vaccination of the 18 kDa protein subunit S. flexneri pili for 4 weeks.…”

Section: Introductionmentioning

confidence: 97%

REACTION BETWEEN Shigella dysentriae SUBUNIT PILI ANTIGEN WITH 18 KDA Shigella flexneri SUBUNIT PILI ANTIBODY

Fitrianingsih, M. Biomed.

2022

El–Hayah

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Shigellosis caused by S. flexneri and S. dysentriae is the leading cause of death in developing countries. Meanwhile, the development of the Shigella vaccine is still ongoing. This study aims to prove the reaction between the 18 kDa S. flexneri pili sub-unit protein's antibodies and with the S. dysentriae pili sub-unit protein antigen. This study used the Western Blot and Dot Blot tests to determine the antibody-antigen reaction both qualitatively and quantitatively. From the study results, it was found that the 18 kDa S. flexneri 18 kDa pili subunit protein was able to recognize the S. dysentriae pili sub-unit protein with a molecular weight of 17 kDa and 23 kDa. The protein subunit pili S. dysentriae with a molecular weight of 17 kDa and 23 kDa probably has an epitope recognized by the antibody to the pili protein subunit S. flexneri 18 kDa. The equivalent zone of the antibody-antigen reaction lies in the concentration of the antibody protein subunit pili 18 kDa S. flexneri titer 1/300 and the concentration of the antigen protein subunit pili 18 kDa S. dysentriae titer 1/20. So that the higher the dilution of the antibody of the 18 kDa S. flexneri pili subunit, the lower the bond with the S. dysentriae pili subunit antigen.

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Combined effects of microplastics and antibiotic-resistant bacteria on Daphnia magna growth and expression of functional genes

Chen,

Wang,

Huang

et al. 2023

Science of The Total Environment

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HAEMAGLUTINATION OF Shigella flexneri SUBUNIT PILI PROTEIN 18 KDA AS A MOLECULE ADHESION IN MICE ENTEROCYTES

Abstract: ABSTRACT

Cited by 3 publications

References 5 publications

Pili Protein 65.5 kDa of Klebsiella pneumoniae Induced a Decrease in IL-10 in Mice

Pili Protein 65.5 kDa of Klebsiella pneumoniae Induced a Decrease in IL-10 in Mice

REACTION BETWEEN Shigella dysentriae SUBUNIT PILI ANTIGEN WITH 18 KDA Shigella flexneri SUBUNIT PILI ANTIBODY

Combined effects of microplastics and antibiotic-resistant bacteria on Daphnia magna growth and expression of functional genes

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