Haemocytes of the clam Tapes philippinarum (Adams &amp; Reeve, 1850): morphofunctional characterisation

Cima, Francesca; Matozzo, Valerio; Marin, María Gabriella; Ballarin, Loriano

doi:10.1006/fsim.2000.0282

Cited by 134 publications

(132 citation statements)

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“…Numerous studies have reported that granulocytes are the main population of hemocytes involved in phagocytosis and ROS production in marine bivalves, although hyalinocytes might exhibit similar phagocytic and oxidative activities (Tripp 1992;Cima et al 2000;Delaporte et al 2003;Goedken and De Guise 2004;Lambert et al 2007;Donaghy et al 2010). Whole hemocytes of oysters from the spill site exhibited a lower total phagocytic activity and lower ROS production than the control oysters (Table 2).…”

Section: Discussionmentioning

confidence: 97%

“…In contrast, the oil spill-damaged oysters exhibited more blast-like cells than the control oysters (14.7 vs. 10.7%, respectively). Blast-like cells are very small agranular cells that are considered stem cells freely circulating in the hemolymph (Cima et al 2000;Aladaileh et al 2007;Matozzo et al 2008;Travers et al 2008;Donaghy et al 2010). Although current knowledge about hemopoiesis in marine bivalves is somewhat limited (Hine 1999;Matozzo et al 2008), multiplication of blastlike cells might have been initiated to mainly restore the decreased granulocyte population.…”

Section: Discussionmentioning

confidence: 99%

“…Hemocytes act as cellular immune eVectors through their capacities to phagocytize, encapsulate, and subsequently degrade invading foreign materials (Montes et al 1995;Chu 2000;Canesi et al 2002). Hemocytes express various hydrolytic enzymes and are able to produce reactive oxygen species (ROS), both of which are involved in the destruction of ingested or encapsulating nonself materials (Adema et al 1991;Toreilles et al 1996;López et al 1997;Cima et al 2000;Bayne et al 2001;Lambert et al 2003;Terahara and Takahashi 2008). Hemocytes are also involved in other vital functions of bivalve biology and physiology, including nutrient transport and digestion, shell and tissue formation, and repair, as well as homeostatic maintenance (Cheng 1996(Cheng , 2000Chu 2000;Beninger et al 2003;Mount et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

et al. 2010

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In marine bivalves, hemocytes support various physiological functions, including immune defense, nutrient transport, shell repair, and homeostatic maintenance. Although the eVects of marine contaminants on the immunological functions of bivalves have been extensively investigated, the impacts of oil spills are not well understood. Therefore, we investigated hemocyte parameters in the PaciWc oyster Crassostrea gigas 13 months after the Hebei Spirit oil spill (December 2007) oV the west coast of Korea. The parameters studied included hemocyte concentration and mortality, relative proportion of hemocyte populations, and immunological functions such as phagocytosis and oxidative activity using Xow cytometry. These immune-related parameters in oysters damaged by the oil spill were also compared to control oysters that were collected from an area unaVected by the spill. The Xow cytometry study indicated that granulocyte population, phagocytic capacity, and reactive oxygen species production in oysters exposed to crude oil 13 months prior were depressed compared to the unexposed control oysters. Our data suggest that immunocompetence in oysters aVected by the oil spill had not fully recovered 1 year after the accident, although more detailed studies on the physiology and disease resistance should be performed.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

et al. 2010

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“…So this kind of cells might be hematopoiesis precursors (Figure 4) [17][18][19][20] . The other kind of cells had positive hTERT and CEA ( Figure 5) expressions after immunohistochemical staining, but expression of CD34 and CD45 was absent.…”

Section: Immunocytochemical and Immunofluorescence Stainingmentioning

confidence: 99%

“…The other kind of cells had positive hTERT and CEA ( Figure 5) expressions after immunohistochemical staining, but expression of CD34 and CD45 was absent. According to the morphology and the outcome of immunohistochemical and immunofluorescence staining, these cells were considered as the disseminated carcinoma cells escaped from the primary neoplasm focus ( Figure 6) [17,[21][22][23] . The first kind of cells was found on all slides, while the other kind of cells was only found on the slides from some gastric carcinoma patients (25/60), the positive rate was about 41.7% (Table 1).…”

Section: Immunocytochemical and Immunofluorescence Stainingmentioning

confidence: 99%

Detection of micrometastasis of gastric carcinoma in peripheral blood circulation

Chen¹,

Chen²,

Wang³

et al. 2004

WJG

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AIM:To detect the micrometastasis of gastric carcinoma in peripheral blood circulation using immunomagnetic beads sorting technique and RT-PCR technique, and to discuss its significance and the difference between the two methods. METHODS:Density gradient centrifugation was used to isolate mononuclear cells from peripheral blood, immunomagnetic beads sorting technique and RT-PCR technique were used to detect the disseminated carcinoma cells. HE, immunocytochemical and immunofluorescence staining were also used to identify the characteristics of the cells separated with immunomagnetic beads sorting technique. RESULTS:Cells expressing cytokeratin were separated and enriched from the peripheral blood specimens of patients suffering from gastric carcinoma or chronic gastritis. After HE staining, two kinds of cells with little cytoplasm were found. Majority of these cells had small and round nuclei, even chromatins and the thickness of nuclear membrane was normal. Immunohistochemical staining indicated that there were CD34 and CD45 expression on the cell membrane of this kind of cells and these cells also showed expressed human telomerase reverse transcriptase by immunofluorescence staining, but the expression of carcinoembryonic antigen was absent. So, these cells might hematopoiesis precursors. Another kind of cells had larger and abnormal nuclei with thicker nuclear membranes. Massed chromatins and polynucleoli were found in the nuclei. These cells expressed human telomerase reverse transcriptase and carcinoembryonic antigen, but CD34 and CD45 were not found on the cell membrane. So, these cells were considered as gastric carcinoma cells escaping from the original focuses and existing in the peripheral blood circulation. Carcinoma cells were found in 25 of 60(41.7%) specimens of peripheral blood from patients with gastric carcinoma, while there were no such cells separated from the blood specimens of chronic gastritis patients. The difference of positive rates of disseminated carcinoma cells between two groups was markedly significant (P<0.005). The expressions of CK20 mRNA in peripheral blood specimens were examinated with RT-PCR. CK20 mRNA was detected from 32 of 60(53.3%) peripheral blood specimens in the group of gastric carcinoma patients, while none of the specimens from patients suffering from chronic gastritis had CK20 mRNA. Significant difference was also found between two groups (P<0.005). Statistic analyses also showed that there was a significant difference between the positive rates of two methods in detecting the disseminated carcinoma cells from the peripheral blood circulation of gastric carcinoma patients (P<0.05). CONCLUSION:The results demonstrated that there were disseminated carcinoma cells in the peripheral blood circulation of some patients with gastric carcinoma. Disseminated carcinoma cells can be detected from the peripheral blood samples with immunomagnetic beads sorting technique and RT-PCR technique. The positive rate of RT-PCR technique is higher than that of immunomagnetic be...

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Circulation, respiration, excretion and osmoregulation

Gosling¹

2015

Marine Bivalve Molluscs

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Haemocytes of the clam Tapes philippinarum (Adams & Reeve, 1850): morphofunctional characterisation

Cited by 134 publications

References 49 publications

Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

Detection of micrometastasis of gastric carcinoma in peripheral blood circulation

Circulation, respiration, excretion and osmoregulation

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