Haemopexin in sheep, mouflon and goat: genetic polymorphism, heterogeneity and partial characterization*

Abstract

SummaryBenzidine staining of starch gels after electrophoresis of sera to which haematin was added revealed polymorphism of haemopexin in sheep, mouflon and goat. In sheep three phenotypes were observed, Hpx A, Hpx AB and Hpx B. Pedigree data support the hypothesis of codominant inheritance from a single locus by two alleles, HpxA and HpxB. Neuraminidase treatment of haemopexin preparations showed that Hpx B covered two variants, B1 and B2, thus indicating genetic control by three alleles (HpxA, HpxB1 and HpxB2). In sheep populations the frequency of HpxB is low. In mouflon, in addition to the two variants that are like those of sheep, absence of haemopexin was observed in some animals, by using starch gel electrophoresis as well as immunoelectrophoresis. In goat, three phenotypes were detected, Hpx A, Hpx AB and Hpx B, differing in migration from those of sheep. Haemopexins of the studied species are heterogeneous. Sialic acid is responsible for electrophoretic heterogeneity of sheep haemopexin. Chemical. composition (amino acid and carbohydrate), molecular weight (56 060) and N‐terminal sequence (Leu‐Pro‐Pro‐) of sheep haemopexin were also determined.

Absence of genetic linkage between A1BG, PGD and HPX loci in rabbits

“…PGD phenotypes in red cell lysates were tested in agarose gel electrophoresis according to Gahne & Juneja (1985). Serum HPX phenotypes were typed by starch gel electrophoresis (Kristjansson 1963;Stratil et al. 1984).…”

Phenotyping of pig α1B‐glycoprotein (PO2) and haemopexin by 1D polyacrylamide gel electrophoresis and immunoblotting

“…Serum samples were separated by horizontal 1D PAGE (Gahne et al 1977) in a modification according to . Serum samples for the detection of HPX were supplemented with haematin before electrophoresis (Stratil et al 1984). Small pieces of Whatman filter paper No.…”