Haemophilus ducreyi Infection in South Florida: A Rare Disease on the Rise?

Tm, Becker; DeWitt, Wallis E.; G, Van Dusen

doi:10.1097/00007611-198702000-00010

Cited by 14 publications

(5 citation statements)

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“…Chancroid, an ulcerating cutaneous infection caused by Haemophilus ducreyi, is one of the most prevalent sexually transmitted diseases in developing countries (61) and is endemic in some areas of the United States (6,8,16,19,36). Chancroid is a recognized cofactor for human immunodeficiency virus transmission (33,40), making H. ducreyi the focus of considerable recent research aimed at understanding the molecular mechanisms of pathogenesis.…”

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Haemophilus ducreyiInfection Causes Basal Keratinocyte Cytotoxicity and Elicits a Unique Cytokine Induction Pattern in an In Vitro Human Skin Model

et al. 1998

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Haemophilus ducreyi is the etiologic agent of the sexually transmitted genital ulcer disease chancroid. Predominantly a cutaneous pathogen, H. ducreyi is present in chancroid ulcers that are characterized by extensive neutrophil accumulation in intraepidermal lesions accompanied by a mononuclear infiltrate in the dermis. We used an in vitro human skin model composed of foreskin fibroblasts and keratinocytes to examine host skin cell interactions with H. ducreyi 35000. Bacteria replicated and persisted in artificial skin for at least 14 days. We observed H. ducreyi inside suprabasal keratinocytes using transmission electron microscopy. Although no bacteria were seen in the basal keratinocyte region, these cells were disrupted in infected cocultures.H. ducreyi infection stimulated increased secretion of interleukin-6 (IL-6) and IL-8 by skin cells. Conversely, tumor necrosis factor alpha and IL-1α levels were not elevated. IL-8 produced in response to H. ducreyi infection may be involved in recruiting polymorphonuclear leukocytes and other inflammatory cells, thereby contributing to the tissue necrosis and ulcer formation characteristic of chancroid.

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confidence: 99%

Haemophilus ducreyiInfection Causes Basal Keratinocyte Cytotoxicity and Elicits a Unique Cytokine Induction Pattern in an In Vitro Human Skin Model

et al. 1998

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“…1). It is the most frequent cause of sexually transmitted endemic in some areas of Africa 4 and the United States 26,33–36 . Hemophilus ducreyi is now well recognized as a cofactor in HIV transmission, 37,38 on account of their frequent coexistence.…”

Section: Etiologymentioning

confidence: 99%

“…It is the most frequent cause of sexually transmitted endemic in some areas of Africa 4 and the United States. 26,[33][34][35][36] Hemophilus ducreyi is now well recognized as a cofactor in HIV transmission, 37,38 on account of their frequent coexistence. This has fascinated investigators in to forming a detailed profile, including the characters, of this organism worldwide.…”

Section: Etiologymentioning

confidence: 99%

“…Subsequently, chancroid infection in Africa has predisposed the risk of perpetuating the already prevalent HIV infection rate either by creating a portal of entry or by increasing the local concentration of cluster differentiating (CD4+) T cells, for which they have a predilection 28–32 . It is the most frequent cause of sexually transmitted endemic in some areas of Africa 4 and the United States 26,33–36 . Hemophilus ducreyi is now well recognized as a cofactor in HIV transmission, 37,38 on account of their frequent coexistence.…”

Section: Epidemiologymentioning

confidence: 99%

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Chancroid: contemporary appraisal

Sehgal¹,

Srivastava²

2003

Int J Dermatology

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“…During the past decade, outbreaks of chancroid have been reported in both Canada and the United States (3,4,8,9,20,21,27). In New York City, outbreaks have been reported yearly beginning in 1981 (10), and the number of cases has been increasing each year since then.…”

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DNA probes for the identification of Haemophilus ducreyi

et al. 1989

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Haemophilus ducreyi ATCC 33922, a virulent, well-characterized strain, was used to construct a genomic library in a bacteriophage expression vector. Three DNA fragments were selected for use as probes on the basis of their ability to encode H. ducreyi-specific proteins, as demonstrated by reactivity with rabbit polyclonal antiserum. With DNA-DNA hybridization, the three probes, labeled with 32p, reacted strongly with 16 strains of H. ducreyi obtained from a variety of sources. Thirty-seven other bacterial isolates, representing 33 different species and including organisms likely to be encountered in the urogenital tract, were also tested with the three probes. Twenty-eight of these isolates, including the genital pathogen Neisseria gonorrhoeae, showed no hybridization with the probes. In addition, herpes simplex virus-infected tissue culture cells and Treponema pallidum-infected rabbit testicular fluid were also completely nonreactive. Nine isolates, six belonging to other Haemophilus species and three belonging to Pasteurella species, reacted weakly with the probes when approximately 3.0 x 107 to 6.0 x 107 CFU was tested. When 105 to 106 CFU of these organisms was tested, the weak reactions could no longer be seen. Yet this number of H. ducreyi still reacted strongly. In fact, the three probes consistently detected 104 CFU of H. ducreyi in pure and mixed cultures and even produced a weak signal when only 103 CFU was present. It is clear from our results that use of these probes will greatly facilitate the laboratory diagnosis of this genital pathogen.

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Haemophilus ducreyi Infection in South Florida: A Rare Disease on the Rise?

Cited by 14 publications

References 0 publications

Haemophilus ducreyiInfection Causes Basal Keratinocyte Cytotoxicity and Elicits a Unique Cytokine Induction Pattern in an In Vitro Human Skin Model

Haemophilus ducreyiInfection Causes Basal Keratinocyte Cytotoxicity and Elicits a Unique Cytokine Induction Pattern in an In Vitro Human Skin Model

Chancroid: contemporary appraisal

DNA probes for the identification of Haemophilus ducreyi

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