Haemorrhagic and permeability increasing effects of ‘Bothrops jararaca’ and other crotalidae venoms as related to amine or kin in release

Vargäftig, B. Boris; Bhargava, N.; Bonta, I. L.

doi:10.1007/bf01970257

Cited by 11 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…12 The oedema induced by Cdt venom was completely abolished by pretreatment with an antihistamine drug. These findings are corroborated by the observation of Vargaftig et al 13 that antihistamine drugs inhibit the increased vascular permeability induced by this venom. Taken together, these results suggest that histamine may be the main mediator of the acute phase of the mild inflammatory response induced by Cdt venom. The main toxin found in Cdt venom is crotoxin which is composed of two subunits, crotapotin and phospholipase A2.14 Phospholipase A2 (PEA2) present in several snakes venoms is endowed with potent oedema forming activity.15-17…”

Section: Effect Of Cdt Venom On Peritoneal Macrophagesupporting

confidence: 80%

The venom of South American rattlesnakes inhibits macrophage functions and is endowed with anti‐inflammatory properties

Silva

Gonçalves

Mariano

1996

Mediators of Inflammation

View full text Add to dashboard Cite

The injection of Crotalus durissus terrificus venom into the foot pad of mice did not induce a significant inflammatory response as evaluated by oedema formation, increased vascular permeability and cell migration. The subcutaneous injection of the venom, or its addition to cell cultures, had an inhibitory effect on the spreading and phagocytosis of resident macrophages, without affecting the viability of the cells. This effect was not observed when the venom was added to cultures of thioglycollate elicited macrophages, but it was able to inhibit these macrophage functions when the cells were obtained from animals injected simultaneously with the venom and thioglycollate. These observations suggest that the venom interferes with the mechanisms of macrophage activation. Leukocyte migration induced by intraperitoneal injection of thioglycollate was also inhibited by previous venom injection. This down-regulatory activity of the venom on macrophage functions could account for the mild inflammatory response observed in the site of the snake bite in Crotalus durissus terrificus envenomation in man.

show abstract

Section: Effect Of Cdt Venom On Peritoneal Macrophagesupporting

confidence: 80%

The venom of South American rattlesnakes inhibits macrophage functions and is endowed with anti‐inflammatory properties

Silva

Gonçalves

Mariano

1996

Mediators of Inflammation

View full text Add to dashboard Cite

show abstract

“…Contrary to earlier suggestions [22], the effects of collagenase were not inhibited but slightly increased after thrombocytopenia had been induced in two dogs by anti-platelet plasma. At doses of 3, 10 or 30 ~zg of collagenase there were no significant differences in total scoring of haemorrhagic activity after each animal was injected with anti-platelet plasma, although the blood platelet count declined from 3.2 • 105 per mm 3 to uncountably low levels in both animals.…”

Section: (1) Effect Of Collagenase On the Lung Surface Of The Dogcontrasting

confidence: 78%

Haemorrhagic and inflammatory properties of collagenase fromC. histolyticum

1976

View full text Add to dashboard Cite

Collagenase from Clostridium histolyticum induced haemorrhages when applied to the surface of dog lung; it exerted a similar effect on mouse lung when injected intrathoracically. Injected into rat paws, bacterial collagenase induced haemorrhage and oedema. Effects of collagenase were prevented by several procedures that inhibit collagenolytic activity (heating at various temperatures and incubation with metal-complexing agents such as EDTA, penicillamine and dithiothreitol). Protein protease inhibitors, dexamethasone and standard acidic anti-inflammatory drugs had only a slight or no effect on collagenase-induced haemorrhages; dexamethasone and acidic anti-inflammatory drugs blocked collagenase-induced oedema. Inhibition of endogenous kinin-releasing mechanisms by administration of hexadimethrine, a recognized inhibitor of the activation of clotting Factor XII, and depletion of kininogen by administration of carrageenin blocked collagenase-induced oedema. Collagenase did not increase permeability of rat skin vessels, nor did it release potential inflammatory mediators, such as bradykinin or prostaglandins, from plasma or platelets. Bacterial collagenase-induced haemorrhage presumably resulted from enzymatic destruction of membranous structures; at least a portion of the inflammatory response may be due to activation of a kinin-like system.

show abstract

“…A factor has been described in B. jararaca venom which contains phospholipases and which may be related to prostaglandin and leukotriene formation, thus causing an increase in vascular permeability, edema and low blood pressure (32). This could account for protein leakage into the tubular spaces.…”

Section: Discussionmentioning

confidence: 99%

Actions of Crotalus durissus terrificus venom and crotoxin on the isolated rat kidney

Monteiro

Silva

Martins

et al. 2001

Braz J Med Biol Res

View full text Add to dashboard Cite

Many studies have reported the occurrence of lethal acute renal failure after snakebites. The aim of the present investigation was to determine alterations in renal function produced by Crotalus durissus terrificus venom and crotoxin as well as the histological alterations induced by these venoms. Isolated kidneys from Wistar rats weighing 240 to 280 g were perfused with Krebs-Henseleit solution containing 6 g% of previously dialyzed bovine serum albumin. The effects of Crotalus durissus terrificus venom and crotoxin were studied on glomerular filtration rate (GFR), urinary flow (UF), perfusion pressure (PP) and percentage sodium tubular transport (%TNa + ). The infusion of Crotalus durissus terrificus venom (10 µg/ml) and crotoxin (10 µg/ml) increased GFR (control 80 = 0.78 ± 0.07, venom 80 = 1.1 ± 0.07, crotoxin 80 = 2.0 ± 0.05 ml g -1 min -1 , P<0.05) and UF (control 80 = 0.20 ± 0.02, venom 80 = 0.32 ± 0.03, crotoxin 80 = 0.70 ± 0.05 ml g -1 min -1 , P<0.05), and decreased %TNa + (control 100 = 75.0 ± 2.3, venom 100 = 62.9 ± 1.0, crotoxin 80 = 69.0 ± 1.0 ml g -1 min -1 , P<0.05). The infusion of crude venom tended to reduce PP, although the effect was not significant, whereas with crotoxin PP remained stable during the 100 min of perfusion. The kidneys perfused with crude venom and crotoxin showed abundant protein material in the urinary space and tubules. We conclude that Crotalus durissus terrificus venom and crotoxin, its major component, cause acute nephrotoxicity in the isolated rat kidney. The current experiments demonstrate a direct effect of venom and crotoxin on the perfused isolated kidney.

show abstract

Haemorrhagic and permeability increasing effects of ‘Bothrops jararaca’ and other crotalidae venoms as related to amine or kin in release

Cited by 11 publications

References 14 publications

The venom of South American rattlesnakes inhibits macrophage functions and is endowed with anti‐inflammatory properties

The venom of South American rattlesnakes inhibits macrophage functions and is endowed with anti‐inflammatory properties

Haemorrhagic and inflammatory properties of collagenase fromC. histolyticum

Actions of Crotalus durissus terrificus venom and crotoxin on the isolated rat kidney

Contact Info

Product

Resources

About