Halal authenticity of gelatin using species-specific PCR

Shabani, Hessam; Mehdizadeh, Mehrangiz; Mousavi, Seyed Mohammad; Dezfouli, Ehsan Ansari; Solgi, Tara; Khodaverdi, Mahdi; Rabiei, Maryam; Rastegar, Hossein; Alebouyeh, Mardawig

doi:10.1016/j.foodchem.2015.02.140

Cited by 82 publications

(58 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With the development of Real-time PCR offering sensitive and specific enough to trace small amounts of target DNA. This technique becomes popular tool to detection of bovine and porcine DNA in gelatin mixtures, gelatin-containing food products and capsule shells (Shabani et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Real-Time Polymerase Chain Reaction for Halal Authentication of Gelatin in Soft Candy

Sepminarti¹,

Sudjadi²,

Wardani³

et al. 2015

Asian J. of Biochemistry

View full text Add to dashboard Cite

Currently, along with the development of science and technology, the diversification of food products occurs in the market. Food products can contain non-halal components like porcine gelatine. One of food suspected to use gelatine is soft candy. Gelatin can be made from pork or beef or other animal. The presence of porcine gelatine in any food products is not allowed for Moslem community, therefore an analytical method offering reliable results must be developed. This study is intended to use Real-Time Polymerase Chain Reaction (RT-PCR) for analysis of porcine gelatine in soft candy. Isolation of DNA was performed with mitochondrial DNA Isolation Kit K280-50 (Bio-Vision). The DNA was analyzed by RT-PCR using primer D-Loop 318. Analysis for the primer specificity was performed on fresh tissue (pig, cows, chickens, goats and rats) and gelatin sources (beef, pigs and catfish). Primer D-loop318 can amplify porcine DNA at the optimum temperature 61.4°C. Repeatability test demonstrated amplification of all positive response samples containing porcine DNA in serial dilution of 10000-1 pg). The Coefficient of Variation (CV) is 6.32%. The repeatability test was also performed on soft candy 100% having CV of 1.06%. The commercial soft candy samples evaluated do not contain porcine DNA.

show abstract

Section: Introductionmentioning

confidence: 99%

Real-Time Polymerase Chain Reaction for Halal Authentication of Gelatin in Soft Candy

Sepminarti¹,

Sudjadi²,

Wardani³

et al. 2015

Asian J. of Biochemistry

View full text Add to dashboard Cite

show abstract

“…The identification of the sample mixtures using the cytochrome b gene could be performed until a 0.1% level [3,12].…”

Section: Discussionmentioning

confidence: 99%

“…Cytochrome b markers have shown good feasibility in detecting species of origin from the sample mixtures. The nucleotide sequence of the cytochrome b gene contains species-specific information and has been used in food safety as well as in forensic investigations in a number of studies [3,4,5,6].…”

Section: Introductionmentioning

confidence: 99%

Identification Human and Animal Blood Mixtures Using Human Cytochrome b Gene

Widodo¹,

Furqoni²,

Yudianto³

et al. 2018

Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change A

View full text Add to dashboard Cite

Abstract-Human and animal blood has a similar composition so it is difficult to distinguish between the two. The aim of this study was to identify human blood from human and animal blood mixtures using the cytochrome b gene that has a species-specific DNA sequence. The DNA extracted from varied human and chicken blood was amplified using a human-specific cytochrome b gene primer. The result showed that 10 % human blood from the mixtures could be identified and the DNA extracted from the human blood could be amplified. The concentration of 0.01 ng still showed the appropriate DNA band. The primer was very sensitive and specific, so it useful for forensic purposes to verify human blood in a blood mixture.

show abstract

“…Additionally, eight commercial gelatin containing food products and eight commercial gelatin capsules were investigated with both methods. After gel electrophoresis of amplified samples it is found that the developed method was very sensitive (as low as 0.1%), specific and affordable for routine analyses of adulteration on gelatin containing foods and capsules (Shabani et al, 2015). Furthermore, multiplex PCR assays can be used with species-specific primers to multiple target detection at the same time.…”

mentioning

confidence: 99%

“…In this technique, a targeted DNA sequence can be amplified precisely. Shabani et al (2015) have developed a new method based on species specific PCR technique to investigate halal authenticity of gelatin. Mitochondrial cyt b gene was targeted and two sets of specific bovine and porcine primers were used.…”

mentioning

confidence: 99%

Origin Determination and Differentiation of Gelatin Species of Bovine, Porcine, and Piscine through Analytical Methods

Eryılmaz

Işık

Demircan

et al. 2017

Turkish JAF Sci.Tech.

View full text Add to dashboard Cite

Gelatin origin determination has been a crucial issue with respect to religion and health concerns. It is necessary to analyze the origin of gelatin with reliable methods to ensure not only consumer choices but also safety and legal requirements such as labeling. There are many analytical methods developed for detection and/or quantification of gelatin from different sources including bovine, porcine and piscine. These analytical methods can be divided into physicochemical, chromatographic, immunochemical, spectroscopic and molecular methods. Moreover, computational methods have been used in some cases consecutively to ensure sensitivity of the analytical methods. Every method has different advantages and limitations due to their own principles, applied food matrix and process conditions of material. The present review intends to give insight into novel analytical methods and perspectives that have been developed to differentiate porcine, bovine and piscine gelatins and to establish their authenticity. Almost every method can be succeeded in origin determination; however, it is a matter of sensitivity in that some researches fail to ensure sufficient differentiation. IntroductionGelatin is a water soluble protein obtained by hydrolysis of collagen to several high molecular weight polypeptides. While the main component of the gelatin is protein (85-92%), it also contains mineral salts and moisture (Baziwane and He, 2003;Schrieber and Gareis, 2007). Gelatin has a great importance in pharmaceutical, cosmetic and especially food industry with its well-known functional properties such as gel formation in jellies, foam formation and stabilization in ice cream, emulsion stabilization in meat products, syneresis stabilization in yogurt (Nhari et al., 2012). Gelatin was produced approximately 326.000 metric tons in 2008 worldwide, and it is estimated to be consumed 395.840 metric tons by the year of 2017 according to Global Industry Analysts, Inc. which is one of the largest and reputed market research companies (Anonymous, 2016).The raw materials used in gelatin production are mainly from bovine and porcine sources: 80% from pig skin, 15% from cattle hide split, the remaining 5 % from bones, fish and other sources (GME, 2016). Gelatin origin determination has been a matter of paramount importance for Muslims, Hindus, Jews, vegetarians and vegans, in that, in Islam and Judaism, porcine derivatives are prohibited to consume, whereas in Hinduism, cow can neither be slaughtered nor consumed. Vegetarians and vegans prefer to eat animal-free foods (Boran et al., 2010;Nhari et al., 2012). Moreover, bovine gelatin has been restricted for human consumption due to Bovine Spongiform Encephalopathy (BSE) outbreak in 1986 in England and other countries (Venien and Levieux, 2005). Therefore, it is necessary to analyze the origin of gelatin with a reliable method. As far as it is concerned; physiochemical, chromatographic, immunochemical, spectroscopic and molecular methods have been developed to determine and differentiate gelatin o...

show abstract

Halal authenticity of gelatin using species-specific PCR

Cited by 82 publications

References 20 publications

Real-Time Polymerase Chain Reaction for Halal Authentication of Gelatin in Soft Candy

Real-Time Polymerase Chain Reaction for Halal Authentication of Gelatin in Soft Candy

Identification Human and Animal Blood Mixtures Using Human Cytochrome b Gene

Origin Determination and Differentiation of Gelatin Species of Bovine, Porcine, and Piscine through Analytical Methods

Contact Info

Product

Resources

About