Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil

Abstract

A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40 T , was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3-15 % (w/v) (optimum, 8 %, w/v), at pH 7.0-9.0 (optimum, pH 8.0) and at 4-60 8C (optimum, 30 8C). It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40 T and the type strains of related species of the genus Halobacillus ranged from 98.8 % (Halobacillus alkaliphilus FP5 T ) to 97.1 % (Halobacillus kuroshimensis IS-Hb7 T ). DNA-DNA hybridization relatedness values between strain NEAU-ST10-40 T and H. alkaliphilus DSM 18525 T , Halobacillus campisalis KCTC 13144 T , Halobacillus yeomjeoni DSM 17110 T , Halobacillus halophilus DSM 2266 T , Halobacillus litoralis DSM 10405 T , Halobacillus dabanensis DSM 18199 T , Halobacillus salinus DSM 18897 T , Halobacillus naozhouensis DSM 21183 T , Halobacillus trueperi DSM 10404 T and Halobacillus salsuginis DSM 21185 T were from 43¡1 to 19¡1 % (mean¡SD). The DNA G+C content was 39.3 mol%. The major fatty acids (.10 %) were anteiso-C 15:0 , anteiso-C 17:0 and iso-C 16:0 , the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40 T is considered to represent a novel species, for which the name Halobacillus andaensis sp. nov. is proposed. The type strain is NEAU-ST10-40 T (5CGMCC 1.12153 T 5DSM 25866 T ).The genus Halobacillus was created by Spring et al. (1996) with the description of two novel species, Halobacillus litoralis and Halobacillus trueperi, and the reclassification of Sporosarcina halophila (Claus et al., 1983) to Halobacillus as the type species of the genus, Halobacillus halophilus.

A UPF0118 family protein with uncharacterized function from the moderate halophile Halobacillus andaensis represents a novel class of Na+(Li+)/H+ antiporter

“…Also, UPF0118 exhibited Na + (Li + )/H + antiport activity mainly under alkaline conditions from pH 8.0 to 9.5 (Fig. 7), which may be related to the adaptation of its host to the extremophilic saline-alkaline conditions, since strain NEAU-ST10–40 T is a moderate halophile with the optimum growth at the 8% (w/v) NaCl concentrations and at pH 8.039.…”

“…In our recent study39, strain NEAU-ST10-40 T , a moderate halophile isolated from Na 2 CO 3 -type saline and alkaline soils in Anda City, China was identified to represent a novel species Halobacillus, Halobacillus andaensis , with the growth range of NaCl concentrations of 3–15% (w/v) (optimum, 8%, w/v) and pH 7.0–9.0 (optimum, pH 8.0), and thus this novel halophilic strain from unique saline-alkaline conditions could have developed sophisticated mechanisms to maintain its intracellular steady osmotic and ionic state. Since almost all halophilic microorganisms have the ability to expel Na + from the interior of the cells using Na + (Li + )/H + antiporters4041, it is very likely that important (even novel) Na + (Li + )/H + antiporter genes exist in this novel strain NEAU-ST10-40 T , a moderate halophile which can tolerate up to 15% (w/v) NaCl.…”

Characterization of a Functionally Unknown Arginine–Aspartate–Aspartate Family Protein From Halobacillus andaensis and Functional Analysis of Its Conserved Arginine/Aspartate Residues

“…The strains and plasmids related to this study are shown in Table 1 . H. andaensis NEAU-ST10-40 T was cultured in a modified S–G liquid medium with the composition as described by Wang et al (2015) . E. coli strain KNabc (Δ nhaA Δ nhaB Δ chaA ; Nozaki et al, 1996 ) and its transformants were cultured in the LBK medium composed of 1.0% tryptone, 0.5% yeast extract, and 87 mM KCl as described by Karpel et al (1988) or KCl-removed LBO (“O” stands for 0 mM KCl) medium solely consisting of 1.0% tryptone and 0.5% yeast extract.…”

“…That has been partially established by the recent report by Meng et al (2017) that a pair of functionally unknown homologous DUF1538 family proteins from the moderate halophile Halomonas zhaodongensis function as a novel two-component Na + (Li + , K + )/H + antiporter. As the type strain of a novel species of Halobacillus andaensis ( Wang et al, 2015 ), NEAU-ST10-40 T is a moderate halophile isolated from unique Na 2 CO 3 -type saline and alkaline conditions, which can grow at the range of NaCl concentrations of 0.5–2.5 M (optimum, 1.4 M) and pH 7.0–9.0 (optimum, pH 8.0). Therefore, this novel halophile may have evolved profound mechanisms for the stability of its intracellular osmotic and ionic state.…”

Draft genome sequence of the moderately halophilic bacterium Halobacillus sp. BBL2006

“…The genus Halobacillus , proposed by Spring et al in 1996 [1] , contains isolates from diverse sources such salt lakes [1] , [2] , [3] , solar salterns [4] , [5] , [6] , [7] , saline soil [8] , [9] , [10] , deep sea methane seeps [11] , sea anemone [12] and mangrove ecosystems [13] , [14] . Many of this group׳s members are of industrial importance for their production of halotolerant extracellular enzymes [15] , [16] .…”