Halocatena marina sp. nov., a novel filamentous halophilic archaeon isolated from marine tidal flat and emended description of the genus Halocatena

Wu, Zhang-Ping; Zheng, Xi-Wen; Sun, Yaping; Wang, Beibei; Hou, Jing; Cui, Heng‐Lin

doi:10.1007/s00792-023-01292-5

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…Strain XZYJT29 T contained the gene cluster encoding for PHA synthesis regulatory protein (PhaR), PHA granule binding protein (PhaP), type IIIA PHA synthase (PhaE and PhaC subunits) and acetoacetyl CoA reductase (PhaB), indicating the probable synthesis of PHAs by the new isolate (Fig. S3) [42]. In addition, strains GSLN9 T and XZYJT29 T contained the genes encoding β-carotene biosynthesis, including CrtB (phytoene synthase) and CrtY (lycopene cyclase), and also Brp, encoding β-carotene dioxygenase that converts β-carotene to retinoids (Fig.…”

Section: Genome Informationmentioning

confidence: 99%

Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov., halophilic archaea isolated from saline soil and an inland solar saltern

Hu,

Ma,

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Two extremely halophilic archaeal strains, GSLN9T and XZYJT29T, were isolated from the saline soil in different regions of western China. Both strains GSLN9T and XZYJT29T have two 16S rRNA genes with similarities of 95.1 and 94.8 %, respectively. Strain GSLN9T was mostly related to the genus Halomicrococcus based on 16S rRNA (showing 91.0–96.0 % identities) and rpoB′ genes (showing 92.0 % identity). Strain XZYJT29T showed 92.1–97.6 % (16S rRNA gene) and 91.4–93.1 % (rpoB′ gene) sequence similarities to its relatives in the genus Halosimplex , respectively. The polar lipid profile of strain GSLN9T included phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulphate (PGS), sulphated mannosyl glucosyl diether (S-DGD-1) and sulphated galactosyl mannosyl glucosyl diether (S-TGD-1), mostly similar to that of Halomicrococcus hydrotolerans H22T. PA, PG, PGP-Me, S-DGD-1 (S-DGD-PA), S2-DGD, S-TGD-1 and an unidentified glycolipid were detected in strain XZYJT29T; this polar lipid composition is similar to those of members of the genus Halosimplex . The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between these two strains and their relatives of the genera Halomicrococcus and Halosimplex were no more than 82, 27 and 80 %, respectively, much lower than the thresholds for species demarcation. Other phenotypic characterization results indicated that strains GSLN9T and XZYJT29T can be differentiated from the current species of the genera Halomicrococcus and Halosimplex , respectively. These results revealed that strains GSLN9T (=CGMCC 1.15215T=JCM 30842T) and XZYJT29T (=CGMCC 1.15828T=JCM 31853T) represent novel species of Halomicrococcus and Halosimplex , for which the names Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov. are proposed.

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Section: Genome Informationmentioning

confidence: 99%

Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov., halophilic archaea isolated from saline soil and an inland solar saltern

Hu,

Ma,

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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“…126–127 of The Halohandbook [ 33 ] works well. Electron microscopy is recommended to examine members with unique cell shapes, such as species of Haloquadratum and Halocatena [ 15 30 ]. For scanning electron microscopic examination, a robust method is as follows: a 0.5 ml fresh culture is fixed overnight at 4 °C by adding glutaraldehyde to a final concentration of 5 %.…”

Section: Morphologymentioning

confidence: 99%

“…Orders, families, and genera with validly published names, not including synonyms, included into the class Halobacteria as of December 2023, based on the classification proposed by Cui et al [4] as rod, coccus, disc, triangular, square, and pleomorphic shapes [1,14]. The recently described Halocatena and Actinarchaeum (name effectively published as Actinoarchaeum and corrected by the List Editors of the International Journal of Systematic and Evolutionary Microbiology) were surprisingly found to be filamentous and produced white spores from mycelia [15,16]. The aerobic and facultatively anaerobic representatives of the Halobacteria are chemoorganotrophic, using amino acids or carbohydrates as carbon sources, while the strictly anaerobic members are chemoorganoheterotrophic, utilizing acetate as electron donor (carbon source) and elemental sulfur as electron acceptor, or chemolithoheterotrophic, using formate or hydrogen as electron donors and elemental sulfur, thiosulfate, or dimethyl sulfoxide as electron acceptors [9,10].…”

Section: Introductionmentioning

confidence: 99%

Proposed minimal standards for description of new taxa of the class Halobacteria

Cui,

Hou,

Amoozegar

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Halophilic archaea of the class Halobacteria are the most salt-requiring prokaryotes within the domain Archaea. In 1997, minimal standards for the description of new taxa in the order Halobacteriales were proposed. From then on, the taxonomy of the class Halobacteria provides an excellent example of how changing concepts on prokaryote taxonomy and the development of new methods were implemented. The last decades have witnessed a rapid expansion of the number of described taxa within the class Halobacteria coinciding with the era of genome sequencing development. The current members of the International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Halobacteria propose these revisions to the recommended minimal standards and encourage the use of advanced technologies in the taxonomic description of members of the Halobacteria. Most previously required and some recommended minimal standards for the description of new taxa in the class Halobacteria were retained in the present revision, but changes have been proposed in line with the new methodologies. In addition to the 16S rRNA gene, the rpoB′ gene is an important molecular marker for the identification of members of the Halobacteria. Phylogenomic analysis based on concatenated conserved, single-copy marker genes is required to infer the taxonomic status of new taxa. The overall genome relatedness indexes have proven to be determinative in the classification of the taxa within the class Halobacteria. Average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity values should be calculated for rigorous comparison among close relatives.

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Genome-based classification of the family Haloferacaceae and description of five novel species of Halobaculum

Tan,

Zhu,

Zhang

et al. 2024

Extremophiles

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Halocatena marina sp. nov., a novel filamentous halophilic archaeon isolated from marine tidal flat and emended description of the genus Halocatena

Cited by 6 publications

References 31 publications

Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov., halophilic archaea isolated from saline soil and an inland solar saltern

Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov., halophilic archaea isolated from saline soil and an inland solar saltern

Proposed minimal standards for description of new taxa of the class Halobacteria

Genome-based classification of the family Haloferacaceae and description of five novel species of Halobaculum

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