Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria

Kharroub, Karima; Quesada, Teresa; Ferrer, Raquel Casesnoves; Fuentes, Susana; Aguilera, Margarita; Boulahrouf, Abderrahmane; Ramos‐Cormenzana, A.; Monteoliva‐Sánchez, Mercedes

doi:10.1099/ijs.0.64272-0

Cited by 48 publications

(21 citation statements)

References 27 publications

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“…Strains similar to members of genus Salicola and Pseudomonas are other bacterial isolates of this study. Salicola is a non-pigmented and extremely halophilic bacteria which was first isolated from shallow salt lakes of Algeria [32]. Pseudomonas halophiles a moderately halophilic bacteria and was first isolated from Great Salt Lake in the USA [33].…”

Section: Volume 56mentioning

confidence: 99%

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Rasooli

Amoozegar

Sepahy

et al. 2016

ILNS

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Abstract. Extremely halophilic diversity of IncheBroun wetland located in the north of Iran was investigated by using culture-dependent methods. Sampling was carried out in May and September 2014. In each sampling 4 distinct regions of wetland were analyzed by using complex media like MGM, JCM168, MH1 and an alkaliphilic medium containing 23% salts. After incubation at 40˚C, a total of 406 isolates and 2.1 × 10 6 CFU/ml were obtained in culture media. Among them 361 isolates were obtained from MGM and 39 isolates from JCM 168, 3 isolates from MH1 and 3 isolates from the alkaliphilic media. Initial morphological, biochemical and physiological tests were performed. Production of 4 hydrolytic enzymes by 45 selected strains was assayed qualitatively. A total of 38, 19 and 6 strains were able to produce lipase, DNase and amylase activity. Protease activity was not observed among strains. As total 45 strains were selected randomly and phylogenetic analysis of 16S rRNA was performed for them. Among selected strains 40 isolated strians belonged to Haloarchaea and were belonged to the genera: Haloarcula(30%), Halorubrum(27.5%), Haloferax(17.5%), Halobellus (10%), Halogeometricum(5.2%), Halobacterium(2.6%), Halolamina(2.6%), Halorhabdus (2.6%) and Halostagnicola (2.6%). Haloarcula and Halorubrum were the dominant populations. A total of 5 strains belonged to domain of Bacteria and were similar to members of Rhodovibrio (40%), Pseudomonas (40%) and Salicola (20%).

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Section: Volume 56mentioning

confidence: 99%

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Rasooli

Amoozegar

Sepahy

et al. 2016

ILNS

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“…aidingense (Cui et al, 2006), Hrr. ezzemoulense (Kharroub et al, 2006), Hrr. orientale (Castillo et al, 2006), Hrr.…”

unclassified

Halorubrum californiense sp. nov., an extreme archaeal halophile isolated from a crystallizer pond at a solar salt plant in California, USA

Pesenti

Sikaroodi

Gillevet

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…alkaliphilum (Feng et al, 2005), Hrr. ezzemoulense (Kharroub et al, 2006), Hrr. orientale (Castillo et al, 2006), Hrr.…”

unclassified

Halorubrum kocurii sp. nov., an archaeon isolated from a saline lake

Gutiérrez

Castillo

Pagaling

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-negative, non-motile, neutrophilic, rod-shaped, extremely halophilic archaeon, designated strain BG-1 T , was isolated from a salt lake, Lake Bagaejinnor, in Inner Mongolia, China. Strain BG-1 T was able to grow at 25-55 6C, required at least 2.5 M NaCl for growth (with an optimum at 3.4 M NaCl) and grew at pH 6.0-9.0 (with an optimum at pH 7.5). Hypotonic treatment with less than 2.0 M NaCl caused cell lysis. Phylogenetic analysis of the almost-complete 16S rRNA gene sequence positioned the isolate within the genus Halorubrum in the family Halobacteriaceae. Strain BG-1 T was most closely related to Halorubrum aidingense 31-hong T (98.8 % sequence similarity), Halorubrum saccharovorum NCIMB 2081 T (98.6 %), Halorubrum lacusprofundi ACAM 34 T (98.6 %) and Halorubrum lipolyticum 9-3 T (98.4 %). However, values for DNA-DNA hybridization between strain BG-1 T and the most closely related members of the genus Halorubrum were below 40 %. Analysis of the polar lipids of strain BG-1 T revealed the presence of mannosyl-2-sulfate-(1-4)-glycosyl-archaeol, the main glycolipid found in neutrophilic species of the genus Halorubrum. The G+C content of the genomic DNA was 69.4 mol% (T m ).Comparison of the phenotypic characteristics of the strain with those of Halorubrum species supported the conclusion that BG-1 T represents a novel species within this genus, for which the name Halorubrum kocurii sp. nov. is proposed. The type strain is BG-1 T (5CECT 7322 T

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Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria

Cited by 48 publications

References 27 publications

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Halorubrum californiense sp. nov., an extreme archaeal halophile isolated from a crystallizer pond at a solar salt plant in California, USA

Halorubrum kocurii sp. nov., an archaeon isolated from a saline lake

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