Hamster oocyte maturation : Inhibition by follicular components

Gwatkin, Ralph B. L.; Andersen, O. F.

doi:10.1016/0024-3205(76)90232-0

Cited by 82 publications

(31 citation statements)

References 17 publications

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“…This conclusion fits well with the observations of Soupart (1975) who showed that normal male pronucleus formation only occurred in human oocytes when estradiol and then estradiol plus 17 aOH progesterone were added sequentially in the culture medium. We have also shown that estradiol and testosterone further cytoplasmic maturation of rabbit oocytes (Thibault et al, 1975 (Wassarman, 1987) generally induces the acrosome reaction which had been shown to occur on the zona (Gwatkin and Andersen, 1976 ;pig, Sz6llbsi and Hunter, 1973 ;mice, Florman and Storey, 1982 ;bovine, Crozet, 1984 ;ovine, Crozet and Dumont, 1984 ; human, Overstreet et al, 19871 …”

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confidence: 87%

“…As pig follicular fluid is easily available and significantly reduced the percentage of spontaneous nuclear maturation of pig, rat (Tsafriri and Channing, 1975) and hamster oocytes (Gwatkin and Andersen, 1976) (1985) reported that purine bases and purine nucleosides, present in porcine and mouse follicular fluids, seem responsible for follicular inhibition of meiosis. When two of these, hypoxanthine and adenosine, are present in the culture medium at the same concentration as in the follicle in vivo, they almost completely inhibit the GVBD in preovulatory mouse oocytes (Eppig et al, 19851.…”

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confidence: 99%

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Mammalian oocyte maturation

Thibault¹,

Szöllősi²,

Gérard³

1987

Reprod. Nutr. Dévelop.

207

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confidence: 87%

mentioning

confidence: 99%

Mammalian oocyte maturation

Thibault¹,

Szöllősi²,

Gérard³

1987

Reprod. Nutr. Dévelop.

207

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“…Several such factors have been reported to contribute to the maintenance of meiotic arrest, such as a peptide of low Mr in the follicular fluid (Gwatkin & Anderson, 1976;Tsafriri et al, 1976), proportions of intrafollicular steroids (Rice & McGaughey, 1981), and hypoxanthine and adenosine (Downs et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Changes in linoleic acid during follicular development and inhibition of spontaneous breakdown of germinal vesicles in cumulus-free bovine oocytes

Homa

Brown²

1992

Reproduction

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Summary. The fatty-acid composition of follicular fluid from small and large developing follicles was analysed and the effects of saturated and unsaturated fatty acids on spontaneous breakdown of germinal vesicles were investigated. Fatty acids were bound to bovine serum albumin and cultured with occytes at 100 \g=m\mol/l. Linoleic acid (18:2) was the only fatty acid tested that significantly inhibited breakdown of germinal vesicles (P < 0\m=.\01). The effect was dose-dependent and was greatest at 50 \g=m\molfatty acid/1 (% breakdown of control, 81\m=.\1\ m=+-\6\m=.\8 vs. 50\g=m\mol linoleic acid/1, 35\m=.\4\ m=+-\7\ m=. \ 3; P < 0\m=.\02). Linoleic acid was the major fatty acid, constituting about a third of the total fatty acid in the follicular fluid; followed by 18\m=.\9 \ m=+-\ 1\m=.\0%and 16\m=.\9 \ m=+-\ 1\m=.\3%oleic acid (18:1) in small and large follicles, respectively. Saturated fatty acids accounted for <30% of the total fatty acid composition. There was a marked absence of tetraenoic acids in small and large follicles. Proportions of linoleic acid were significantly lower in follicular fluid from large follicles (31\m=.\1 \m=+-\1\m=.\2%of total fatty acid) than from small follicles (34\m=.\8 \m=+-\0\m=.\7%of total fatty acid) (P < 0\m=.\05) and there was a significant inverse correlation between follicle diameter and percentage of linoleic acid in the follicular fluid (r= \m=-\0\m=.\6966; P < 0\m=.\05). There was no significant alteration in any other fatty acid during follicular development. The results suggest that (i) a critical relative concentration of linoleic acid may be required for maintenance of meiotic arrest in bovine oocytes and (ii) the decline in the proportion of linoleic acid during follicular development may contribute to the relief of the inhibition of breakdown of germinal vesicles.

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“…LH is known to stimulate the preovulatory follicle to produce glycosaminoglycans which accumulate on the surface and in the intercellular spaces of the cumulus cells (Eppig, 1979). Sato et aL (1986) (Gwatkin and Andersen, 1976). Under our conditions the inhibitory factor from porcine cumulus cells does not inhibit maturation in mouse oocytes but it is able to exert its effect on cattle oocytes.…”

Section: Resultsmentioning

confidence: 63%

“…In addition, LH has been reported to alleviate the inhibitory action of porcine follicular fluid or of its purified fractions (Tsafriri & Channing, 1975), of bovine or hamster follicular fluid (Gwatkin & Andersen, 1976), of rat granulosa cell conditioned medium, as well as that of cocultured with rat granulosa cells (Tsafriri et al, 1977). Hence the ability of LH to partly overcome the inhibition of meiosis by a cumulus cell factor described in the present study lends support to the view that this factor may have a physiological role in the regulation of meiosis.…”

Section: Resultsmentioning

confidence: 99%