Haploid identification using tropicalized haploid inducer progenies in maize

Ribeiro, Cauê; Pereira, Francielly de Cássia; Filho, Lamartine da Nóbrega; Rezende, Breno Alvarenga; Dias, Kaio Olímpio das Graças; Braz, Guilherme Tomaz; Ruy, Murilo Candido; Silva, M.B.; Cenzi, Gisele; Techio, Vânia Helena; Souza, João Cândido de

doi:10.1590/1984-70332018v18n1a3

Cited by 21 publications

(31 citation statements)

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“…(2015), and Ribeiro et al. (2018). Although these are efficient tools, they require specialized skills and involve continuous costs and special preparation techniques.…”

Section: Identification Of Haploidsmentioning

confidence: 95%

“…Ribeiro et al. (2018) attempted but failed to prepare metaphase C slides, which indicated the need to conduct further optimization studies.…”

Section: Identification Of Haploidsmentioning

confidence: 99%

“…Ribeiro et al. (2018) used flow cytometry to validate the putative haploids. This method of haploid identification is the most efficient based on accuracy and number of samples that can be studied on a daily basis but is a costly method.…”

Section: Identification Of Haploidsmentioning

confidence: 99%

“…Anthocyanin pigments imparted by the Navajo gene also vary in color intensity from year to year (Ribeiro et al., 2018). The R1‐nj gene expression pattern was also reported to be variable and show incomplete penetrance (Belicuas et al., 2007).…”

Section: Phenotypic Markersmentioning

confidence: 99%

“…Changes in the R1‐nj expression pattern were also attributed to the fact that anthocyanin pigment is also naturally expressed in the pericarp tissues of some germplasm sources (Chaikam et al., 2016). Kernel type (dent or flint) also influences the expression as it was reported that dent kernels express more intense anthocyanin pigmentation because of the R1‐nj gene (Ribeiro et al., 2018). Both F 1 and F 2 populations could be used for haploid induction.…”

Section: Phenotypic Markersmentioning

confidence: 99%

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Doubled haploids in maize: Development, deployment, and challenges

Maqbool¹,

Beshir²,

Khokhar³

2020

Crop Science

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Haploids are naturally produced in maize (Zea mays L.) at different rates and can also be induced through different methods. Haploids are used to develop doubled haploids (DHs), which have many potential uses. The development of DH lines in maize involves haploid induction, haploid identification, chromosome doubling, and field sowing for self‐pollination of D0 plants. Different potential methods are used for haploid induction, in‐vivo maternal haploid induction being the most prevalent. Haploid induction is highly reliant on the unambiguous identification of haploids among a mixture of different ploidies. Haploid identification is facilitated by visual morphological markers, chromosome counting, flow cytometry, molecular markers, and many other approaches. Chromosome doubling may be achieved by spontaneous doubling or by induction with different antimicrotubular treatments. Among the potential uses of DH lines are the development of inbred lines, genomic selection (GS), quantitative trait loci (QTL) mapping, and unlocking new genetic variations. Although DH technology can potentially accelerate maize breeding, it still faces challenges at each step of DH line development. This article aims to highlight the importance, procedural steps, potential opportunities, and key challenges in DH line development in maize.

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“…(2015), and Ribeiro et al. (2018). Although these are efficient tools, they require specialized skills and involve continuous costs and special preparation techniques.…”

Section: Identification Of Haploidsmentioning

confidence: 95%

“…Ribeiro et al. (2018) attempted but failed to prepare metaphase C slides, which indicated the need to conduct further optimization studies.…”

Section: Identification Of Haploidsmentioning

confidence: 99%

Section: Identification Of Haploidsmentioning

confidence: 99%

Section: Phenotypic Markersmentioning

confidence: 99%

Section: Phenotypic Markersmentioning

confidence: 99%

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