Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line

Abstract

Abstract:Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. In these species, megagametophyte cultures have been widely used to obtain haploid callus and somatic embryogenic lines. One of the main problems associated with tissue culture is the potential genetic instability of the regenerants. Because of this, chromosomal stability of the callus and/or somatic embryos should also be assessed. To this end, chromosome counting, flow cytometry and genotyping using microsatellites have been reported. Here, we present an overview of the work done in conifers, with special emphasis on the production of a haploid cell line in maritime pine (Pinus pinaster L.) and the use of a set of molecular markers, which includes Single Nucleotide Polymorphisms (SNPs) and microsatellites or Single Sequence Repeats (SSRs), to validate chromosomal integrity confirming the presence of all chromosomic arms.

Identification and high-throughput genotyping of single nucleotide polymorphism markers in a non-model conifer (Abies nordmanniana (Steven) Spach)

“…We used haploid megagametophytes as a source of DNA for the full genome sequencing. Haploid tissues are useful for the genomic study of species with large genomes 50 . They can be used as a first step in sequence complexity reduction.…”

Reproduction and genetic accuracy during somatic embryogenesis in Larix sibirica

“…The genetic instability of larch species in vitro cultures as well as of other plant taxa is well known (von Aderkas, Anderson, 1993;Burg et al, 2007). Nevertheless, some studies indicate that during somatic embryogenesis in some Pinaceae species genetic variation was not detected and regenerant genetics was the same as in the original explant (Mo et al, 1989;Hеlmersson et al, 2004;Arrillaga et al, 2014;Cabezas et al, 2016;Ahn, Choi, 2017).…”