Harmonic generation by yeast cells in response to low-frequency electric fields

Nawarathna, Dharmakeerthi; Claycomb, J. R.; Cárdenas, Gustavo; Gardner, J.; Warmflash, David; Miller, Jr Jh; Widger, W.

doi:10.1103/physreve.73.051914

Cited by 18 publications

(15 citation statements)

References 31 publications

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“…This low-pass behaviour is consistent with recent experimental findings indicating the absence of harmonic cell response to EM carriers in the mobile-telephone frequency bands [41,42], and the presence of such responses to (very) low frequency fields [43,44].…”

Section: Numerical Resultssupporting

confidence: 79%

Nonlinear Interaction of Electromagnetic Radiation at the Cell Membrane Level: Response to Stochastic Fields

Vita

Croce²,

Pinto³

et al. 2011

PIER B

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Abstract-A general rigorous analytic framework for computing the transmembrane potential shift resulting from the nonlinear voltagecurrent membrane relationship in response to wideband stochastic electromagnetic radiation is outlined, based on Volterra functional series. The special case of an insulated cylindrical cell with HodgkinHuxley membrane in an infinite homogeneous medium is worked out in detail, for the simplest case where the applied electric is normal to the cell axis, and independent from the axial coordinate. Representative computational results for a zero-average stationary band-limited white Gaussian incident field are illustrated and briefly discussed.

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Section: Numerical Resultssupporting

confidence: 79%

Nonlinear Interaction of Electromagnetic Radiation at the Cell Membrane Level: Response to Stochastic Fields

Vita

Croce²,

Pinto³

et al. 2011

PIER B

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“…Because the SQUID is non-contacting, requiring no measurement electrodes, this technique enables nonlinear dielectric spectroscopy of living cell suspensions without any spurious polarization effects caused by dipole layers at the pick up electrode-medium interface. Details of the nonlinear harmonic response measurement setup for cells in suspension and sample preparations have been reported [9]- [11].…”

Section: Harmonic Response Of Cell Suspensionsmentioning

confidence: 99%

SQUID-Based Biosensor for Probing Ion Transporters in Cell Suspensions and Tissue

Nawarathna

Claycomb

Cárdenas

et al. 2007

IEEE Trans. Appl. Supercond.

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We have developed a SQUID-based technique to probe membrane-bound proteins, such as cation transporters. The method involves excitation of a biological sample with a sinusoidal electric field (0-5 V/cm, 10-300 Hz), detection, with a SQUID magnetometer, of the magnetic field produced by ac currents in the tissue or cell suspension, and calculation of the Fast Fourier Transform (FFT) of the measured response to obtain a spectrum. We find that the harmonic response of budding yeast cells (S. cerevisiae, 10 8 cells/ml) is sensitive to sodium metavanadate, an inhibitor of a proton pump known as H + -ATPase, glucose, a substrate of H + -ATPase, and gramicidin, a membrane depolarizer. SQUID-based field and frequency dependent harmonic response measurements are also made of a live earthworm (Lumbricus terrestris). Use of the SQUID significantly reduces spurious harmonics and distortion of the excitation field by the measurement electrodes.

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“…Nevertheless, the THD performance is good enough to register the nonlinear dielectric properties of biological suspensions, according to previous reports. [1][2][3][4][5]15,[19][20][21] …”

Section: Discussionmentioning

confidence: 99%

“…: ϩ54͑264͒4211700/ext 313. issue. [16][17][18][19][20][21][22] At the moment, there are no reports about spectrometer that provides true information about the linear/ nonlinear dielectric properties of aqueous suspensions. This paper presents and evaluates a tetrapolar nonlinear spectrometer.…”

Section: Introductionmentioning

confidence: 99%

Design and evaluation of a fast Fourier transform-based nonlinear dielectric spectrometer

Treo

Felice

2009

Review of Scientific Instruments

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Nonlinear dielectric spectroscopy of micro-organism is carried out by applying a moderate electrical field to an aqueous sample through two metal electrodes. Several ad hoc nonlinear spectrometers were proposed in the literature. However, these designs barely compensated the nonlinear distortion derived from the electrode-electrolyte interfaces (EEI). Moreover, the contribution of the suspension is masked by the effect of the nonlinearity introduced by the electrode contacts. Conversely, the nonlinear capability of a commercial tetrapolar analyzer has not been fully investigated. In this paper a new nonlinear tetrapolar spectrometer is proposed based on a commercial linear apparatus and ad hoc control and signal processing software. The system was evaluated with discrete electronic phantoms and showed that it can measure nonlinear properties of aqueous suspension independently of the presence of EEI (ANOVA test, p>0.001). It was also tested with real aqueous samples. The harmonics observed in the current that circulates through the sample reveals useful information about the transfer function of the sample. The total harmonic distortion was computed for linear mediums. Values lower than -60 dB suggest that the system has enough capability to perform nonlinear microbiological analysis. Design specifications, sources of interference, and equipment's limitations are discussed.

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Harmonic generation by yeast cells in response to low-frequency electric fields

Cited by 18 publications

References 31 publications

Nonlinear Interaction of Electromagnetic Radiation at the Cell Membrane Level: Response to Stochastic Fields

Nonlinear Interaction of Electromagnetic Radiation at the Cell Membrane Level: Response to Stochastic Fields

SQUID-Based Biosensor for Probing Ion Transporters in Cell Suspensions and Tissue

Design and evaluation of a fast Fourier transform-based nonlinear dielectric spectrometer

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