Has the time come to include low-level laser photobiomodulation as an adjuvant therapy in the treatment of impaired endometrial receptivity?

Abstract

Low-level laser therapy (LLLT) has been used in photobiomodulation to promote healing, regenerate, and restore tissue function. Women with persistent thin endometrium were assumed to encounter diminished activity in the regenerative and functional capacity of their endometrium. The aim of this study was to examine the ability of LLLT in 635 nm wavelength to enhance the proliferation and gene expression of in vitro cultured endometrial cells. Single (SE) versus multiple exposures (ME) to LLLT were examined in the study groups and compared to controls. A fluence dose of 4.27 J/cm for 16 min was given once in the SE or divided in three equal sessions in days 2, 4, and 6 of the culture in ME. Cellular response was evaluated by measuring viable cell numbers and surface area. Pattern of MUC1, ITGA5, ITGB3, LIF, and PTEN gene expression was assessed using the qRT-PCR. Greater numbers of cells were found in both study groups (P < 0.001) as compared to controls. The surface area of cells at the end of culture phase was highly significant (P < 0.001) in ME when compared to SE and controls. A statistically significant difference was found in terms of gene expression in both irradiated groups (P < 0.001) as compared to controls, although greater difference in PTEN tumour suppressor gene (Phosphatase and tensin homolog) expression was toward ME. The introduction of LLLT to the armamentarium of infertility will serve as a new adjuvant therapy in this field. The current study proofed that LLLT was able to increase the proliferative and functional capacity of cultured endometrial cells.

Cellular homeostasis, implantation window and unexplained infertility: role of phosphatase and tensin homolog deleted on chromosome 10

“…The results suggested that LLLT may serve as a new complementary therapy in improving endometrial receptivity and regeneration by inducing up regulation of PTEN gene expression. 16 In the present study, we found significantly higher number of Ki67 immunopositive nuclei in GE and stroma of midsecretory endometrial biopsies from infertile women as compared to fertile controls, suggesting increased proliferative activity in women with unexplained infertility during implantation window.…”

The Treatment of Complementary and Alternative Medicine on Female Infertility Caused by Endometrial Factors

“…Low-level laser therapy (LLLT) is also a photobiometric therapy that can absorb lasers at the electronic level without producing thermal effects [ 173 ]. El Faham et al [ 174 ] conducted in vitro culture of 40 infertile women's endometrium to explore whether LLLT could enhance its proliferative ability. The studies have shown that when the wavelength of LLLT is 635 nm, the expression of endometrial receptivity genes can be induced, and the ability of endometrial cells to differentiate and regenerate is the strongest.…”

“…Lowlevel laser therapy (LLLT) is also a photobiometric therapy that can absorb lasers at the electronic level without producing thermal effects [173]. El Faham et al [174] conducted in vitro culture of 40 infertile women's endometrium to explore whether LLLT could enhance its proliferative ability.…”

Photobiomodulation therapy at 632 nm wavelength ameliorates intrauterine adhesion via activation of cAMP/PKA/CREB pathway

“…8,9 Furthermore, the few studies focusing on female reproductive diseases have suggested that PBM increased endometrial cell proliferation and the expression of endometrial receptivity genes in vitro. 10,11 Thus, PBM seems to have great potential for reducing fibrosis and promoting endometrial regeneration. However, whether PBM attenuates endometrial fibrosis and impaired fertility caused by IUA remains unclear.…”

“…The potential anti‐fibrotic effects of PBM at various wavelengths on diverse cells and tissues have been reported 8,9 . Furthermore, the few studies focusing on female reproductive diseases have suggested that PBM increased endometrial cell proliferation and the expression of endometrial receptivity genes in vitro 10,11 . Thus, PBM seems to have great potential for reducing fibrosis and promoting endometrial regeneration.…”