HAUS Augmin-Like Complex Subunit 1 Influences Tumour Microenvironment and Prognostic Outcomes in Glioma

Abstract

Background. The expression of HAUS Augmin-like complex subunit 1 (HAUS1), a protein-coding gene, is low in normal samples among various cancers with pan-cancer analysis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. However, the detailed expression pattern of HAUS1 and its correlation with immune infiltration in glioma (LGG and GBM) (LGG: low-grade glioma; GBM: glioblastoma) remain unknown. Therefore, in this study, we examined the role and prognostic value of HAUS1 in glioma. Methods. Transcriptional expression data of HAUS1 were collected from the CGGA and TCGA databases. The Kaplan–Meier analysis, univariate and multivariate Cox analyses, and receiver operating characteristic (ROC) curves were used to analyse the clinical significance of HAUS1 in glioma. The STRING database was used to analyse protein-protein interactions (PPI), and the “ClusterProfiler” package was used for functional enrichment analysis to examine the possible biological roles of HAUS1. In addition, the HAUS1 promoter methylation modification was analysed using MEXPRESS, and the association between HAUS1 expression and tumour-infiltrating immune cells was investigated using CIBERSORT. Results. Based on the data retrieved from TCGA (703 samples) and CGGA (1018 samples), an elevated expression of HAUS1 was observed in glioma samples, which was associated with poorer survival of patients, unfavourable clinical characteristics, 1p/19q codeletion status, WHO grade, and IDH mutation status. Furthermore, multivariate and univariate Cox analyses revealed that HAUS1 was an independent predictor of glioma. HAUS1 expression level was associated with several tumour-infiltrating immune cells, such as Th2 cells, macrophages, and activated dendritic cells. The outcomes of ROC curve analysis showed that HAUS1 was good to prognosticate immune infiltrating levels in glioma with a higher area under the curve (AUC) value (AUC = 0.974). Conclusions. HAUS1 was upregulated and served as a biomarker for poor prognosis in patients with glioma. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells, which had high infiltration levels. Therefore, these findings suggest that HAUS1 is a potential biomarker for predicting the prognosis of patients with glioma and plays a pivotal role in immune infiltration in glioma.

A Prognostic Risk Model of a Novel Oxidative Stress-Related Signature Predicts Clinical Prognosis and Demonstrates Immune Relevancy in Lung Adenocarcinoma

“…Although many clinical studies have confirmed that multitargeted drugs and immunotherapy can prolong the overall survival (OS) and improve the objective response rate (ORR) of LUAD patients [3][4][5], the rapid progression of the disease due to multi-drug resistance is currently very difficult. Few targeted treatment options [6][7][8][9]. With the indepth research in many aspects [10,11], it is beneficial to discover small molecule inhibitors.…”

The significance of HAUS1 and its relationship with immune microenvironment in hepatocellular carcinoma

“…HAUS1 is significant for the spindle assembly as the first subunit of the Human Augmin Complex and some researchers have found that HAUS1 and HAUS6 are similar, interact with NEDD1, target spindle microtubules 33 , participate in forming cellular spindle filaments, cell mitosis, and regulating cell cycles 34 . Current evidence suggested that HAUS1 was closely associated with tumor formation and might contribute to tumor progression 35 . Analysis of TCGA, GTEx, GEO and HPA databases showed that HAUS1 was significantly highly expressed in HCC, which was associated with a worse prognosis and reduced survival among patients.…”

Single‐cell and bulk RNA‐sequencing reveals mitosis‐involved gene HAUS1 is a promising indicator for predicting prognosis and immune responses in prostate adenocarcinoma (PRAD)

“…Meanwhile, another phenomenon called checkpoint adaptation might happen. Adaptation of the checkpoint allowed cells to withdraw from mitosis without spindle formation or chromosome segregation (Fujita et al, 2016; Yao et al, 2022). The cells that exited mitosis due to the checkpoint adaptation might re‐enter S phase through a process named endoreduplication, which would produce polyploid cells.…”