HAUS5 Is A Potential Prognostic Biomarker With Functional Significance in Breast Cancer

Abstract

BackgroundBreast cancer (BRCA) has become the most frequently appearing, lethal, and aggressive cancer with increasing morbidity and mortality. Previously, it was discovered that the HAUS5 protein is involved in centrosome integrity, spindle assembly, and the completion of the cytoplasmic division process during mitosis. By encouraging chromosome misdivision and aneuploidy, HAUS5 has the potential to cause cancer. The significance of HAUS5 in BRCA and the relationship between its expression and clinical outcomes or immune infiltration remains unclear.MethodsPan-cancer was analyzed by TIMER2 web and the expression differential of HAUS5 was discovered. The prognostic value of HAUS5 for BRCA was evaluated with KM plotter and confirmed with Gene Expression Omnibus (GEO) dataset. Following that, we looked at the relationship between the high and low expression groups of HAUS5 and breast cancer clinical indications. Signaling pathways linked to HAUS5 expression were discovered using Gene Set Enrichment Analysis (GSEA). The relative immune cell infiltrations of each sample were assessed using the CIBERSORT algorithm and ESTIMATE method. We evaluated the Tumor Mutation Burden (TMB) value between the two sets of samples with high and low HAUS5 expression, as well as the differences in gene mutations between the two groups. The proliferation changes of BRCA cells after knockdown of HAUS5 were evaluated by fluorescence cell counting and colony formation assay.ResultHAUS5 is strongly expressed in most malignancies, and distinct associations exist between HAUS5 and prognosis in BRCA patients. Upregulated HAUS5 was associated with poor clinicopathological characteristics such as tumor T stage, ER, PR, and HER2 status. mitotic prometaphase, primary immunodeficiency, DNA replication, cell cycle related signaling pathways were all enriched in the presence of elevated HAUS5 expression, according to GSEA analysis. The BRCA microenvironment’s core gene, HAUS5, was shown to be related with invading immune cell subtypes and tumor cell stemness. TMB in the HAUS5-low expression group was significantly higher than that in the high expression group. The mutation frequency of 15 genes was substantially different in the high expression group compared to the low expression group. BRCA cells’ capacity to proliferate was decreased when HAUS5 was knocked down.ConclusionThese findings show that HAUS5 is a positive regulator of BRCA progression that contributes to BRCA cells proliferation. As a result, HAUS5 might be a novel prognostic indicator and therapeutic target for BRCA patients.

Comprehensive analysis of the correlation of the pan-cancer gene HAUS5 with prognosis and immune infiltration in liver cancer

“…As shown in Fig. 1 C, HAUS5 mRNA levels were significantly higher in glioblastoma (GBM) 17 , brain lower grade glioma (LGG), lung adenocarcinoma (LUAD), colon adenocarcinoma (COAD), breast invasive carcinoma (BRCA) 16 , esophageal carcinoma (ESCA), kidney renal papillary cell carcinoma (KIRP), stomach adenocarcinoma (STAD), prostate adenocarcinoma (PRAD), uterine corpus endometrial carcinoma (UCEC), head and neck squamous cell carcinoma (HNSC), kidney renal clear cell carcinoma (KIRC), lung squamous cell carcinoma (LUSC), LIHC, thyroid carcinoma (THCA), rectum adenocarcinoma (READ), bladder urothelial carcinoma (BLCA) and cholangiocarcinoma (CHOL) tissues than in normal tissues. Due to the small normal sample size of some cancers, we integrated the GTEx and TCGA datasets with log2(x + 1) expression value and analyzed the differences of HAUS5 expression in 27 cancer types.…”

“…Although it is known the augmin complex plays an important role in the correct assembly of the spindle, few studies have been conducted on HAUS5. A recent study suggested that HAUS5 is a potential biomarker in breast cancer 16 . However, the role of HAUS5 in hepatocellular carcinoma remains unknown and deserves further investigation.…”

Comprehensive Bioinformatics Analysis Reveals PTPN1 (PTP1B) Is a Promising Immunotherapy Target Associated with T Cell Function for Liver Cancer

“…To investigate the potential biological function and pathway, we frst screened the DEGs between the low-and high-PTPN1 expression groups using the R package "limma." Te genes with log 2 |fold change (FC)| larger than one and adjusted p value (q value) lower than 0.05 were considered signifcant DEGs [14]. Next, these DEGs were used to perform GO and KEGG pathway enrichment analysis.…”

“…Next, these DEGs were used to perform GO and KEGG pathway enrichment analysis. GO terms and KEGG pathways with q value lower than 0.05 were signifcantly enriched in DEGs [14]. Furthermore, we also utilized GSEA pathway analysis on PTPN1 by the KEGG gene sets.…”

“…Ten, we explored the association between the expression of PTPN1 and the clinical characteristics and survival of patients with liver cancer in the TCGA and ICGC databases. To explore whether the biological function and signal pathway of PTPN1 is related to immune responses, three functional and pathway enrichment methods were conducted [13][14][15]. Tese methods were the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis on diferentially expressed genes (DEGs) between low-and high-PTPN1 expression groups, the Gene Set Enrichment Analysis (GSEA), and the GO and KEGG analysis on PTPN1-correlated genes.…”