Pathogenic Vibrio spp. are Gram-negative bacteria and can infect many fish and shellfish species including olive flounder (Paralichthys olivaceus) (Lee et al., 2021). Such bacteria cause vibriosis via gastric invasion in marine species (Chen et al., 2003), exhibiting typical hemorrhagic septicemia, lethargy and dark skin lesions in infecting olive flounder. Vibrio spp. have caused mass mortalities and extensive economic losses of olive flounder aquaculture industry in