Hb Hope: A Rare Variant of Haemoglobin-Forged a Rise in HbA1c on Immunoturbidimetric Assay

Singh, Bratati; Behera, Debasis; Mehta, Neelam; Das, Sankar

doi:10.1007/s12291-013-0406-0

Cited by 8 publications

(11 citation statements)

References 9 publications

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“…Therefore, the possibility of interference by a silent Hb like Hb hope should always be discussed while analyzing the abnormally raised HbA1c value. Moreover, several methods for estimation of glycosylated Hb should be used while dealing with aberrant raised HbA1c value 6 . The laboratories should have alternative assay systems such as glycated albumin to assist the management of the DM patient with a hemoglobin variant.…”

Section: Discussionmentioning

confidence: 99%

Variant hemoglobin (Hb hope) may enhance glycosylated hemoglobin (HbA1c) results of cation exchange high performance liquid chromatography

Kapoor¹

2018

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Section: Discussionmentioning

confidence: 99%

Variant hemoglobin (Hb hope) may enhance glycosylated hemoglobin (HbA1c) results of cation exchange high performance liquid chromatography

Kapoor¹

2018

jmscr

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“…The same mutation at the analogous position in the beta subunit of HbA (N108K) is known to decrease oxygen affinity . In addition, LtEc also has three other substitutions relative to EfEc (H103Q in the A subunit, along with Q132F and G145D in the D subunit) that have been shown to decrease the oxygen affinity of HbA (D94H, P124Q, and G136D, respectively) . Overall, any or all of these isolated mutations could be responsible for the relatively low oxygen affinity of LtEc, but future mutational studies would be needed to confirm this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Direct comparison of oligochaete erythrocruorins as potential blood substitutes

Zimmerman

DiIusto

Dienes

et al. 2017

Bioengineering & Transla Med

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While many blood substitutes are based on mammalian hemoglobins (e.g., human hemoglobin, HbA), the naturally extracellular hemoglobins of invertebrates (a.k.a. erythrocruorins, Ecs) are intriguing alternative oxygen carriers. Specifically, the erythrocruorin of Lumbricus terrestris has been shown to effectively deliver oxygen in mice and rats without the negative side effects observed with HbA. In this study, the properties of six oligochaete Ecs (Lumbricus terrestris, Eisenia hortensis, Eisenia fetida, Eisenia veneta, Eudrilus eugeniae, and Amynthas gracilis) were compared in vitro to identify the most promising blood substitute candidate(s). Several metrics were used to compare the Ecs, including their oxidation rates, dissociation at physiological pH, thermal stability, and oxygen transport characteristics. Overall, the Ecs of Lumbricus terrestris (LtEc) and Eisenia fetida (EfEc) were identified as promising candidates, since they demonstrated high thermal and oligomeric stability, while also exhibiting relatively low oxidation rates. Interestingly, the O2 affinity of LtEc (P 50 = 26.25 mmHg at 37 °C) was also observed to be uniquely lower than EfEc and all of the other Ecs (P 50 = 9.29–13.62 mmHg). Subsequent alignment of the primary sequences of LtEc and EfEc revealed several significant amino acid substitutions within the D subunit interfaces that may be responsible for this significant change in O2 affinity. Nonetheless, these results show that LtEc and EfEc are promising potential blood substitutes that are resistant to oxidation and denaturation, but additional experiments will need to be conducted to determine their safety, efficacy, and the effects of their disparate oxygen affinities in vivo.

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“…1 Hb I Philadelphia, Hb Hope and Hb Sherwood Forest were associated with falsely elevated HbA 1C levels. 10,26,28,29 The first case of heterozygous Hb Sherwood Forest was reported in a pregnant female having anemia and another case of homozygous Hb Sherwood Forest had erythrocytosis. Majority of the cases were reported from Pakistan and Iran.…”

Section: Delta Gene Variants and Double Heterozygous Variantsmentioning

confidence: 99%

Wide spectrum of novel and rare hemoglobin variants in the multi‐ethnic Indian population: A review

Thaker,

Mahajan,

Mukherjee

et al. 2024

Int J Lab Hematology

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The hemoglobin (Hb) variants are qualitative abnormalities due to production of structurally abnormal globin proteins. They are categorized based on the type of mutation present in the α1, α2, β, Gγ, Aγ and δ globin genes. So far, more than 1550 Hb variants are reported in the database. They could lead to Hb polymerization, Hb instability, altered oxygen affinity and decreased oxygen‐carrying capacity of Hb or have no clinical manifestations. In India, ethnic diversity, consanguinity, regional variations and migration result in the presence of different Hb variants. We have compiled all the variants of α, β and δ globin chains in heterozygous, homozygous and in compound heterozygous forms reported from India in the last 52 years. Of the 63 rare and novel hemoglobin variants reported from India, 22 were α‐globin chain variants, 37 were β‐globin chain variants and 4 were δ‐globin chain variants. Twelve novel Hb variants (Hb J Rajappan, Hb Koya Dora, Hb Rampa, Hb Godavari, Hb Chandigarh, Hb D Agri, Hb Lucknow, Hb Vellore, Hb Midnapore, Hb Bijnor, Hb A2Tianhe and Hb A2Saurashtra) were identified among persons of Indian origin. Majority of them were picked up on HPLC. Some of the variants like Hb Titusville, Hb Shimonoseki, Hb Chandigarh, Hb D Agri, Hb Yaizu and Hb Vellore eluted in the HbS window whereas variants like HbD Iran, Hb St. Louis, Hb G Coushata, HbM Saskatoon, Hb Lucknow, Hb Grange‐Blanche and Hb Tianshui showed falsely elevated HbA2. Hence, careful and systematic investigations are required to identify them.

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Hb Hope: A Rare Variant of Haemoglobin-Forged a Rise in HbA1c on Immunoturbidimetric Assay

Cited by 8 publications

References 9 publications

Variant hemoglobin (Hb hope) may enhance glycosylated hemoglobin (HbA1c) results of cation exchange high performance liquid chromatography

Variant hemoglobin (Hb hope) may enhance glycosylated hemoglobin (HbA1c) results of cation exchange high performance liquid chromatography

Direct comparison of oligochaete erythrocruorins as potential blood substitutes

Wide spectrum of novel and rare hemoglobin variants in the multi‐ethnic Indian population: A review

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