HBO1 directs histone H4 specific acetylation, potentiating mechano-transduction pathways and membrane elasticity in ovarian cancer cells

Quintela, Marcos; Sieglaff, Douglas H.; Gazze, Andrea; Zhang, Aijun; González, Deyarina; Francis, Lewis; Webb, Paul; Conlan, R. Steven

doi:10.1016/j.nano.2019.01.017

Cited by 33 publications

(33 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…OVCAR.3 wide lower expression of MT1MMP, COLL11A1, vimentin,0.40, 0.33, 0.45, respectively, on 3D compared to 2D culture seems to suggest the acquisition of an even less metastatic phenotype with the only exception of E-cad which was also downregulated 0.45 times in 3D compared to 2D (p > 0.05) (Figure 7). Finally, YAP1, a transcriptional factor and mechanotransducer involved in the initiation, progression, and metastasis of several cancers (Zanconato et al, 2016;Quintela et al, 2019), was downregulated 0.49 times in SKOV.3 and 0.55 in OVCAR.3 cells cultured on 3D scaffolds compared to 2D culture, suggesting that multidimensional culturing methods strongly influence ovarian cancer gene expression compared to 2D systems (p > 0.05) (Figure 7).…”

Section: Ovca Metastasis-related Markers Expression In 3d Scaffolds Cmentioning

confidence: 99%

Marine Collagen Substrates for 2D and 3D Ovarian Cancer Cell Systems

Paradiso

Fitzgerald

Yao

et al. 2019

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

A fundamental structural component of extracellular matrix in all connective and interstitial tissue, collagen is the most abundant protein in the human body. To date, mammalian collagens sources represent the golden standard for multiple biomedical applications, while marine-derived collagens have largely been used in industry (food, pharmaceutical, and cosmetic), with little use in research and clinical applications. Herein we demonstrate the effective use Rhizostoma pulmo jellyfish collagen, a source of biocompatible, sustainable collagen for 2D and 3D cell culture, addressing the global drive for technological developments that result in the replacement of animals and their derived products in research. Jellyfish collagen harbors similar structural features mammalian collagen type I, despite differing slightly in amino acid content. Jellyfish collagen supports ovarian cancer (OvCa) cell line proliferation, cellular morphology and expression of epithelial to mesenchymal transition markers, supporting the use of R. pulmo as a non-mammalian collagen cell culture substrate. Furthermore, R. pulmo collagen is effective in 3D device fabrication such as sponges where it mimics tissue architecture complexity. OvCa cells migrated and differentiated within the R. pulmo collagen 3D scaffolds confirming its suitability for advanced cell culturing applications, providing an excellent alternative to mammalian collagen sources for the culture of human cells.

show abstract

Section: Ovca Metastasis-related Markers Expression In 3d Scaffolds Cmentioning

confidence: 99%

Marine Collagen Substrates for 2D and 3D Ovarian Cancer Cell Systems

Paradiso

Fitzgerald

Yao

et al. 2019

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Because an acetyltransferase affects gene expression in a wide spectrum, it is of interest to identify other potential transcription factors targets of KAT7 in MCP-1 and RANTES regulation. Apart from transcription factors, histone H3 and H4 are acetylated by KAT7 (25)(26). Another question remains unresolved is if the histone acetylation by KAT7 contributes to MCP-1 and RANTES gene transcription.…”

Section: Discussionmentioning

confidence: 99%

“…It has demonstrated that a number of KLFs bind to co-factors that possess histone acetyltransferase (HAT) activity, such as cAMP response element binding-binding protein (CBP), p300, and p300/CBP-associated factor (P/CAF) 7 , 22 , 23 . As a co-factor, lysine acetyltransferase 7 (KAT7, also known as HBO1 or MYST2) is also a member of the HAT family 24 , and KAT7 is involved in gene transcription via acetylation of histone H3/H4 and non-histone proteins 25 - 27 . Besides, KAT7, as a regulator of inflammatory cytokine gene transcription, not only affects IL-1β, IL-6, and IL-10 synthesis in THP-1 monocytes upon lipopolysaccharide exposure, but also induces IL-6 expression in synovial fibroblasts via epigenetic mechanism 28 , 29 .…”

Section: Introductionmentioning

confidence: 99%

KLF6 Acetylation Promotes Sublytic C5b-9-Induced Production of MCP-1 and RANTES in Experimental Mesangial Proliferative Glomerulonephritis

Gong

Liu

et al. 2020

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Rat Thy-1 nephritis (Thy-1N) is an experimental mesangial proliferative glomerulonephritis (MsPGN) for studying human MsPGN. Although sublytic C5b-9 complex formation on glomerular mesangial cells (GMCs) and renal MCP-1 and RANTES production in rats with Thy-1N have been proved, the role and mechanism of MCP-1 or RANTES synthesis in GMCs induced by sublytic C5b-9 are poorly elucidated. In this study, we first found the expression of transcription factor (KLF6), co-activator (KAT7) and chemokines (MCP-1 and RANTES) was all up-regulated both in renal tissue of Thy-1N rats (in vivo) and in sublytic C5b-9-induced GMCs (in vitro). Further in vitro experiments revealed that KLF6 bound to MCP-1 promoter (-297 to-123 nt) and RANTES promoter (-343 to-191 nt), leading to MCP-1 and RANTES gene transcription. Meanwhile, KAT7 also bound to the same region of MCP-1 and RANTES promoter in a KLF6-dependent manner, and KLF6 was acetylated by KAT7 at lysine residue 100, which finally promoted MCP-1 and RANTES expression. Moreover, our in vivo experiments discovered that knockdown of renal KAT7 or KLF6 gene obviously reduced MCP-1 and RANTES production, GMCs proliferation, ECM accumulation, and proteinuria secretion in Thy-1N rats. Collectively, our study indicates that sublytic C5b-9-induced MCP-1 and RANTES synthesis is associated with KAT7-mediated KLF6 acetylation and elevated KLF6 transcriptional activity, which might provide a new insight into the pathogenesis of rat Thy-1N and human MsPGN.

show abstract

“…A previous study demonstrated the effect of indole-3carbinol (I3C), indicating that I3C may inhibit metastasis and invasion of OVCAR-3 [32]. HBO1-directed histone H4 specific acetylation, potentiating the membrane elasticity of ovarian cancer cells, and HBO1 mRNA expression were significantly higher in OVCAR-3 cells than in the HOSEpiC control [33]. The different roles of lamin A and lamin C were also elucidated in the metastasis of ovarian cancer cells HO-8910 and SKOV-3.…”

Section: Viscoelastic Properties Of Ovarian Cancer Cellsmentioning

confidence: 99%

Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy

Chen¹,

Zeng²,

Ruan³

et al. 2020

Beilstein J. Nanotechnol.

View full text Add to dashboard Cite

The mechanical properties of cells could serve as an indicator for disease progression and early cancer diagnosis. This study utilized atomic force microscopy (AFM) to measure the viscoelastic properties of ovarian cancer cells and then examined the association with the invasion of ovarian cancer at the level of living single cells. Elasticity and viscosity of the ovarian cancer cells OVCAR-3 and HO-8910 are significantly lower than those of the human ovarian surface epithelial cell (HOSEpiC) control. Further examination found a dramatic increase of migration/invasion and an obvious decease of microfilament density in OVCAR-3 and HO-8910 cells. Also, there was a significant relationship between viscoelastic and biological properties among these cells. In addition, the elasticity was significantly increased in OVCAR-3 and HO-8910 cells after the treatment with the anticancer compound echinomycin (Ech), while no obvious change was found in HOSEpiC cells after Ech treatment. Interestingly, Ech seemed to have no effect on the viscosity of the cells. Ech significantly inhibited the migration/invasion and significantly increased the microfilament density in OVCAR-3 and HO-8910 cells, which was significantly related with the elasticity of the cells. An increase of elasticity and a decrease of invasion were found in OVCAR-3 and HO-8910 cells after Ech treatment. Together, this study clearly demonstrated the association of viscoelastic properties with the invasion of ovarian cancer cells and shed a light on the biomechanical changes for early diagnosis of tumor transformation and progression at single-cell level.

show abstract

HBO1 directs histone H4 specific acetylation, potentiating mechano-transduction pathways and membrane elasticity in ovarian cancer cells

Cited by 33 publications

References 49 publications

Marine Collagen Substrates for 2D and 3D Ovarian Cancer Cell Systems

Marine Collagen Substrates for 2D and 3D Ovarian Cancer Cell Systems

KLF6 Acetylation Promotes Sublytic C5b-9-Induced Production of MCP-1 and RANTES in Experimental Mesangial Proliferative Glomerulonephritis

Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy

Contact Info

Product

Resources

About