2004
DOI: 10.1161/01.atv.zhq0504.1403
|View full text |Cite
|
Sign up to set email alerts
|

HDL 3 Induces Cyclooxygenase-2 Expression and Prostacyclin Release in Human Endothelial Cells Via a p38 MAPK/CRE-Dependent Pathway: Effects on COX-2/PGI-Synthase Coupling

Abstract: Objective-In endothelial cells, cyclooxygenase-1 (COX-1) and COX-2 both contribute to prostacyclin production. Recent findings suggest that COX-2 contributes significantly to systemic prostacyclin synthesis in humans; whether COX-2 inhibition is related to an increased cardiovascular risk is undergoing debate. HDLs have been shown to increase prostacyclin synthesis, thus in the present study we investigated the molecular mechanisms involved in this effect in endothelial cells. Methods and Results-HDL 3 (30 g/m… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

4
75
0

Year Published

2006
2006
2016
2016

Publication Types

Select...
3
2
2

Relationship

0
7

Authors

Journals

citations
Cited by 89 publications
(79 citation statements)
references
References 50 publications
4
75
0
Order By: Relevance
“…The higher prevalence of CHD and angina seen in the CC genotype may be due to reduced HDLcholesterol levels, since HDL-cholesterol is effective in countering the formation of these diseases by increasing COX-2 expression within smooth muscle [28,29]. The HDL-cholesterol induced COX-2 expression is directed towards prostacyclin production, a potent vasodilator [29].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The higher prevalence of CHD and angina seen in the CC genotype may be due to reduced HDLcholesterol levels, since HDL-cholesterol is effective in countering the formation of these diseases by increasing COX-2 expression within smooth muscle [28,29]. The HDL-cholesterol induced COX-2 expression is directed towards prostacyclin production, a potent vasodilator [29].…”
Section: Discussionmentioning
confidence: 99%
“…The higher prevalence of CHD and angina seen in the CC genotype may be due to reduced HDLcholesterol levels, since HDL-cholesterol is effective in countering the formation of these diseases by increasing COX-2 expression within smooth muscle [28,29]. The HDL-cholesterol induced COX-2 expression is directed towards prostacyclin production, a potent vasodilator [29]. Therefore, low HDL-cholesterol levels result in a lower COX-2 expression in the CC genotype which may lead to increased blood pressure and subsequent coronary heart disease manifesting in angina, however this would be more likely be expected of the GG genotype, since endothelial dysfunction is increased with COX-2 activity [5,9].…”
Section: Discussionmentioning
confidence: 99%
“…Total RNA was extracted and underwent reverse transcription as described (4,17). Three μl of cDNA was amplified by real-time quantitative PCR with 1X SYBER Green universal PCR mastermix (BioRad) (4).…”
Section: Methodsmentioning
confidence: 99%
“…The melting peaks of the amplicons were as expected (data not shown). The primers used, the amplicon size and the melting temperature have been described previously (4). Each sample was analyzed in duplicate using the IQ™-Cycler (Bio-Rad).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation