Headspace Solid-Phase Micro-Extraction Versus Hydrodistillation of Volatile Compounds from Leaves of Cultivated Mentha Taxa: Markers of Safe Chemotypes

Abstract

Various mint taxa are widely cultivated and are used not only for medicinal purposes but also in cosmetic and industrial applications. The development of new varieties or cultivars of mint generates difficulties in their correct identification and safe use. Volatile organic compounds (VOCs) from the leaves of seven different taxa of the genus Mentha obtained by hydrodistillation (HD) and headspace solid-phase microextraction (HS-SPME) were analyzed using gas chromatography–mass spectrometry (GC-MS). Principal component analysis (PCA) was also performed. Comparative GC-MS analysis of the obtained extracts showed similarity in the major compounds. PCA data allowed the separation of two groups of chemotypes among the analyzed mints, characterized by the abundance of piperitenone oxide and carvone. Two out of seven analyzed taxa were not previously examined for VOC profile, one was examined only for patent application purposes, and six out of seven were investigated for the first time using the HS-SPME technique. The presented analysis provides new data on the abundance and qualitative characterization of VOCs in the studied mint plants and on the safety of their use, related to the possibility of the presence of potentially toxic components. HS-SPME is a valuable method to extend the characterization of the VOC profile obtained by hydrodistillation.

Exploring Volatile Organic Compounds in Rhizomes and Leaves of Kaempferia parviflora Wall. Ex Baker Using HS-SPME and GC–TOF/MS Combined with Multivariate Analysis

“…Some temperature-sensitive volatiles can be degraded when high temperatures are used during the analysis, which is eliminated in the HS-SPME. Adam et al (2022) also confirmed that more volatile components were detected using HS-SPME than hydro-distillation in Mentha species [ 66 ]. Moreover, semi-volatiles, such as oxygenates and some hydrocarbons, were also detected in this study, but had not been reported in previous studies.…”

“…In the characterization of VOCs from plant matrices, the sample preparation and detection techniques are critical steps in achieving optimal results [ 65 , 66 ]. Nowadays, headspace solid-phase microextraction (HS-SPME) sample preparation coupled with gas chromatography/time-of-flight mass spectrometry (GC-TOF-MS) has been widely employed for metabolic profiling of volatiles from highly complex plant matrices.…”

“…Nowadays, headspace solid-phase microextraction (HS-SPME) sample preparation coupled with gas chromatography/time-of-flight mass spectrometry (GC-TOF-MS) has been widely employed for metabolic profiling of volatiles from highly complex plant matrices. In the past, sample preparation of VOCs analysis relied on different extraction methods, including hydrodistillation (HD), supercritical fluid extraction (SFE), and solvent extractions [ 66 ]. These methods are time-consuming; utilize high amounts of solvents, resulting to excessive solvent waste; and causing some temperature-sensitive volatiles to be degraded at high temperatures during extraction [ 67 ].…”

Peanut oil odor enhances the immunomodulatory effect on immunosuppressed mice by regulating the cAMP signaling pathway via the brain–spleen axis

“…The reported method was used with slight modifications. 28,29 The volatile compounds were extracted using a solid-phase (SPME) microextraction device, configured with 50/30 μm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) (Supelco, Bellefonte, PA, U.S.A.) fibers. First, 5 g of peanut oil and 2 g of peppermint oil were weighed into a 20 mL headspace vial and sealed with an aluminum cap and Teflon silicone septum.…”

The Influence of Microwave Treatments on Bioactive Compounds and Antioxidant Capacity of Mentha piperita L.

“…Most studies focus on the application of microwaves as a technique for extracting essential oils from peppermint [ 13 , 16 , 24 , 25 ]. The novelty of this study consists in the application of microwaves with the aim of making the extraction process of bioactive compounds such as polyphenols and peppermint pigments more efficient.…”