Headspace, Volatile and Semi-Volatile Organic Compounds Diversity and Radical Scavenging Activity of Ultrasonic Solvent Extracts from Amorpha fruticosa Honey Samples

Jerković, Igor; Marijanović, Zvonimir; Kezić, Janja; Gugić, Mirko

doi:10.3390/molecules14082717

Cited by 42 publications

(40 citation statements)

References 25 publications

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“…Indeed it is perhaps to soon postulate biological origins and roles for these VOC's as part of a stress-sensitive response in breath. At this stage it is helpful to note that benzaldehyde is a common food component and occurs naturally in fruits like apples and honey [31,30] likewise terpenes occur naturally in foodstuffs, [29]. These components may be due to dietary intake and may not have an endogenous origin.…”

Section: Multi-variate Analysismentioning

confidence: 99%

The effect of a paced auditory serial addition test (PASAT) intervention on the profile of volatile organic compounds in human breath: a pilot study

et al. 2013

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This study sought to identify if detectable changes in human breath profiles may be observed following a psychological intervention designed to induce stress, a paced auditory serial addition test (PASAT). Breath samples were collected from 22 participants (10 male and 12 female) following a double cross-over randomized design with two experimental interventions. One intervention required participants to listen to classical music chosen to be neutral. The other intervention required participants to undertake a PASAT that induced cardiovascular responses consistent with acute stress. Both interventions also involved two sequences of cognitive function tests. Blood-pressure and heart-rate were recorded throughout each intervention and distal breath samples were collected onto Tenax® TA/Carbograph 1 thermal desorption tubes, using an adaptive breath sampler. Samples were collected before and after the PASAT. Breath samples were analysed by thermal desorption gas chromatography-mass spectrometry. Data registration using retention indexing and peak deconvolution followed by partial least-squares discriminant analysis identified six stress sensitive compounds. A principal components analysis model based on these components generated a model that predicted post-PASAT versus post-neutral intervention samples with a sensitivity of 83.3% and a selectivity of 91.6% for females, compared to 100% sensitivity and 90% selectivity for males. Of the six compounds indole, 2-hydroxy-1-phenylethanone, benzaldehyde, and 2-ethylhexan-1-ol were identified on the basis of mass spectral, retention indexing and confirmation against pure standards. 2-methylpentadecane was tentatively identified from mass spectral and retention indexing, whilst one component has yet to be assigned, although the mass spectrum is indicative of a terpene. Indole and 2-methylpentadecane concentrations increased in response to the PASAT intervention, while the other compounds reduced in their abundance in human breath, possibly as a result of ventilation effects.

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Section: Multi-variate Analysismentioning

confidence: 99%

The effect of a paced auditory serial addition test (PASAT) intervention on the profile of volatile organic compounds in human breath: a pilot study

et al. 2013

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“…The fibre was conditioned prior to use according to the manufacturer instructions by inserting into the GC injector port. Procedure of the HS-SPME was described in [11] [29]. A total of 5 ml of honey/H 2 O soln.…”

Section: Experimental Partmentioning

confidence: 99%

“…USE was performed in an ultrasound cleaning bath (Transsonic Typ 310/H, Germany) by the mode of indirect sonication, at the frequency of 35 kHz at 25 AE 38 as described in [11] [29]. Forty grams of Salix spp.…”

Section: Experimental Partmentioning

confidence: 99%

Volatile Composition Screening of Salix spp. Nectar Honey: Benzenecarboxylic Acids, Norisoprenoids, Terpenes, and Others

Jerković

Marijanović

2010

Chemistry & Biodiversity

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Salix spp. nectar honey volatiles of Croatian origin were analyzed by headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE), followed by gas chromatography and mass spectrometry (GC, GC/MS). Isolated volatiles were found in the honey headspace and extracts with almost exclusive distribution of several abundant compounds (e.g., phenylacetic acid, pinocembrin, 8-hydroxy-4,7-dimethylcoumarin, and 3-hydroxy-trans-β-damascone in the extracts, or safranal and lilac alcohols in the headspace). Comparison with Croatian Salix spp. honeydew honey revealed similarities regarding distribution of important shikimate pathway derivatives (e.g., high percentage of phenylacetic acid) and several norisoprenoids (α-isophorone and 4-oxoisophorone). On the other hand, distinct features of this honey were occurrence of compounds such as pinocembrin, 8-hydroxy-4,7-dimethylcoumarin, phenylacetonitrile, norisoprenoids (major ones: 3-hydroxy-trans-β-damascenone and trans-β-damascone), more pronounced variability of linalool-derived compounds, as well as the abundance of 3-methylpropanoic acid, 3-methylbutanoic acid, 2-methylpentanoic acid, and 3-methylpentan-1-ol.

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“…However, none of the single methods proposed proved to be simple, rapid, and objective. Recently, our research group suggested a two-way approach for the isolation of the honey volatile compounds, i.e., headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE) [7] [8]. With this approach, it was possible to obtain a more detailed insight into the honey volatile and semi-volatile compounds and to minimize the risks of compound discrimination due to their partial extraction.…”

mentioning

confidence: 99%

Volatile Compounds of Asphodelus microcarpusSalzm. et Viv. Honey Obtained by HS‐SPME and USE Analyzed by GC/MS

Jerković

Tuberoso

Kasum

et al. 2011

Chemistry & Biodiversity

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Chemical analysis of Asphodelus microcarpus Salzm. et Viv. honey is of great importance, since melissopalynology does not allow the unambiguous determination of its botanical origin. Therefore, the volatile compounds of eight unifloral asphodel honeys have been investigated for the first time. The honey extracts were obtained by headspace solid-phase microextraction (HS-SPME) and ultrasonicsolvent extraction (USE) and analyzed by GC and GC/MS. In the honey headspace, 31 volatile compounds were identified with high percentages of 2-phenylacetaldehyde (2; 14.8–34.7%), followed by somewhat lower percentages of methyl syringate (1; 10.5–11.5%). Compound 2 is not a specific marker of the botanical origin of the honey, but its high percentage can be emphasized as headspace characteristic of asphodel honey. The extraction solvent for all the samples was selected after extracting a representative sample with pentane, Et(2)O, pentane/Et(2)O 1:2 (v/v), and CH(2)Cl(2) . Compound 1 was the major constituent of all the USE extracts (46.8–87.0%). According to these preliminary results, all the honey samples were extracted by USE with the solvent pentane/Et(2)O 1:2. A total of 60 volatile compounds were identified with 1 as predominant compound (69.4–87.0%), pointing out 1 as Asphodelus honey volatile marker.

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Headspace, Volatile and Semi-Volatile Organic Compounds Diversity and Radical Scavenging Activity of Ultrasonic Solvent Extracts from Amorpha fruticosa Honey Samples

Cited by 42 publications

References 25 publications

The effect of a paced auditory serial addition test (PASAT) intervention on the profile of volatile organic compounds in human breath: a pilot study

The effect of a paced auditory serial addition test (PASAT) intervention on the profile of volatile organic compounds in human breath: a pilot study

Volatile Composition Screening of Salix spp. Nectar Honey: Benzenecarboxylic Acids, Norisoprenoids, Terpenes, and Others

Volatile Compounds of Asphodelus microcarpusSalzm. et Viv. Honey Obtained by HS‐SPME and USE Analyzed by GC/MS

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