Healthy in Vitro Propagation by Meristem Tip Culture of Helleborus Niger¿s Selected Clone for Cut Flower

Poupet, R.; Cardin, L.; Henri, Amvam Zollo Paul; Onesto, J. P.

doi:10.17660/actahortic.2006.725.37

Cited by 6 publications

(10 citation statements)

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“…The axillary buds of four hellebore species (H. argutifolius, H. foetidus, H. niger, H. orientalis) were also used successfully for in vitro initiation on modified MS medium enriched with 2iP and BAP [17] or on MS medium supplemented with BAP, 2iP and NAA [19]. Poupet et al [11] produced virus-indexed H. niger plants only from the apical meristem on medium containing: kinetin, GA 3 , and IBA. Apical buds of H. niger seedlings were isolated and established on medium supplemented with BAP and GA 3 [16].…”

Section: Discussionmentioning

confidence: 99%

“…Apical buds of H. niger seedlings were isolated and established on medium supplemented with BAP and GA 3 [16]. [11,16,17]. The multiplication rate was significantly higher for H. niger than for H. argutifolius, H. foetidus, and H. orientalis [17].…”

Section: Discussionmentioning

confidence: 99%

“…It is also important as pot and cut flowers. It is one of the most popular Christmas cut flowers cultivated in southern France and exported from France to Germany, the Netherlands, Switzerland, and Belgium [11]. Also, the other species are popular as garden perennials: H. orientalis, H. viridis, H. lividus, H. foetidus, H. purpurascens, and H. argutifolius.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro propagation of many plants has played a very important role in rapid multiplication of cultivars and the production of healthy plants. Relatively little tissue culture research has been published on the micropropagation of hellebores [11,[15][16][17][18][19][20]. The shoot multiplication efficacy of hellebores is influenced by several factors, such as: type of initial explants, genotype, growth regulators, and environmental factors [11,16,17].…”

Section: Introductionmentioning

confidence: 99%

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Effect of different sucrose and nitrogen salt levels in the medium and temperature on in vitro propagation of Helleborus niger L.

Gabryszewska

2015

Acta Agrobot

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Helleborus niger L. is a rhizomatous, herbaceous perennial with overwintering, divided, basal leaves. The objective of the study was to investigate the influence of different levels of sucrose (10, 20, 30, 40, 50, 60, 70, and 80 g l−1) and nitrogen salts (25%, 50%, and 100% according to MS medium) as well as temperature (15°C, 20°C) on in vitro multiplication and rooting and ex vitro acclimatization of H. niger. The growth and multiplication of axillary shoots were performed on modified MS medium supplemented with various growth regulators (2iP, BAP and kinetin – each at a concentration of 1.0 mg l−1, GA3 2.5 mg l−1). For the induction of roots, the medium was supplemented with IBA 1 mg l−1 and NAA 0.1 mg l−1. Rooted plants were transplanted in a peat–perlite substrate (4:1) in a heated greenhouse for ex vitro acclimatization. The multiplication rate of H. niger shoots, in vitro rooting, and ex vitro acclimatization were strongly dependent on the sucrose/nitrogen salt relationship in the medium. The highest multiplication rate of axillary shoots (3.7) was found at a temperature of 15°C or 20°C, on the medium with cytokinins and GA3 supplemented with sucrose 20–30 g l−1 and nitrogen salts at 50%. Sucrose at a concentration of 50 g l−1 strongly stimulated the number of roots per microplant (5.8–6.0) on the media with a reduced level of nitrogen salts (25% and 50%) when the temperatures were 20°C and 15°C, respectively. The plants rooted on the media with a high sucrose/nitrogen salt ratio showed acclimatization rates which ranged from 82% to 100%. Morphological observation of plantlets revealed obvious differences in leaf shape and size and the architecture of the root system as well as differences in the developmental stages of shoots grown on media with different sucrose and nitrogen salt concentrations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of different sucrose and nitrogen salt levels in the medium and temperature on in vitro propagation of Helleborus niger L.

Gabryszewska

2015

Acta Agrobot

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“…In a nursery environment, the chilling of young plants stimulated growth (Lowder et al, 2010) and accelerated flowering time (Christiaens et al, 2012). As it turned out, microshoot multiplication and rooting in vitro is more effective at a temperature of around 15 o C (Seyring, 2002;Poupet et al, 2006;Dhooghe and van Labeke, 2007;Beruto and Curir, 2009;Gabryszewska, 2014). Establishing of this condition can be a problem due to the cost of cooling.…”

Section: Introductionmentioning

confidence: 99%

Burkholderia phytofirmansPsJN promotes in vitro rooting and acclimatization ofHelleborus

Orlikowska¹,

Nowak²,

Ogórek³

2017

Acta Hortic.

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Helleborus is a valuable ornamental plant, flowering in early spring or winter. Its hybrid cultivars can be propagated vegetatively, including in vitro via axillary shoots. One of the obstacles preventing effective micropropagation is the necessity for culture to be kept at a temperature of around 15 o C, which additionally increases costs. Knowledge obtained up to date shows that inoculating plants with bacteria Burkholderia phytofirmans PsJN can increase their tolerance to the non-optimal growth temperature. In this study, we tested whether the bacterium, which is known to produce a relatively high amount of IAA and demonstrates ACC deaminase activity, was able to stimulate Helleborus root formation at a temperature of 23 o C.In our experiment, the microshoots cultured without auxin and not inoculated with PsJN rooted in 83% with 1.7 roots of 0.6 cm long. The microshoots, which were induced to root with auxins IBA 3 mg/L and NAA 1 mg/L but not inoculated, were rooted in 94% with 2.0 roots of 1.1 cm long. A similar result was obtained for the microshoots not rooted on auxin containing medium but inoculated with PsJN -95% rooted shoots with 2.3 roots of 1.2 mm long. The microshoots that were induced to root on the auxins containing medium and then inoculated with PsJN were rooted in 100% with 6.9 roots of 2.1 cm long. Almost all microplants from this treatment acclimatized to greenhouse conditions and grew vigorously, then flowered after winter precooling in a mildly heated greenhouse.

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Micropropagation of Helleborus through Axillary Budding

Beruto

Viglione

Bisignano

2012

Methods in Molecular Biology

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Helleborus genus, belonging to the Ranunculaceae family, has 20 species of herbaceous perennial flowering plants. The commercial exploitation of this plant is dependent on the selection and propagation of appropriate lines. High propagation rate could be accomplished by using a suitable tissue culture method enabling the rapid introduction of valuable selections in the market. However, in vitro cultivation of Helleborus is still very difficult. Thereby the development of reliable in vitro propagation procedures is crucial for future production systems. Axillary buds cultured on agar-solidified Murashige and Skoog medium supplemented with 1 mg/L benzyladenine, 0.1 mg/L β-naphthoxyacetic acid, and 2 mg/L isopentenyl adenine develop shoots after 16 weeks of culture under 16 h light regime, 50-60 μmol/s/m(2), and 19 ± 1°C. The multiplication rate ranges from 1.4 to 2.1. However, the genotype and the number of subcultures affect the efficiency of the micropropagation process. The rooting of shoots is about 80% in solidified MS medium containing 1 mg/L 1-naphthaleneacetic acid and 3 mg/L indole-3-butyric acid. The described protocol provides information which can contribute to the commercial production of Helleborus plants.

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Healthy in Vitro Propagation by Meristem Tip Culture of Helleborus Niger¿s Selected Clone for Cut Flower

Cited by 6 publications

References 0 publications

Effect of different sucrose and nitrogen salt levels in the medium and temperature on in vitro propagation of Helleborus niger L.

Effect of different sucrose and nitrogen salt levels in the medium and temperature on in vitro propagation of Helleborus niger L.

Burkholderia phytofirmansPsJN promotes in vitro rooting and acclimatization ofHelleborus

Micropropagation of Helleborus through Axillary Budding

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