Heart Histopathology and Mitochondrial Ultrastructure in Aged Rats Fed for 24 Months on Different Unsaturated Fats (Virgin Olive Oil, Sunflower Oil or Fish Oil) and Affected by Different Longevity

Navarro-Hortal, María D.; Ramírez-Tortosa, César L.; Varela-López, Alfonso; Romero-Márquez, Jose M.; Ochoa, Julio J.; Ramírez-Tortosa, M. C.; Forbes-Hernández, Tamara Y.; Granados-Principal, Sergio; Battino, Maurizio; Quiles, José L.

doi:10.3390/nu11102390

Cited by 14 publications

(3 citation statements)

References 59 publications

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“… 2019 ), and sunflower oil (Navarro-Hortal et al. 2019 ), also achieve a similar effect in anti-ageing. Therefore, the present study investigated the active functions on the antioxidative stress of LBSO and showed the effects of L. barbarum on protecting individuals from ageing by antioxidation and anti-inflammation; however, the active components of L. barbarum are yet elusive (Gao et al.…”

Section: Introductionmentioning

confidence: 92%

SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

Zhou

Wang

Zhao

et al. 2021

Pharmaceutical Biology

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Context Lycium barbarum L. (Solanaceae) seed oil (LBSO) exerts LBSO exerts protective effects in the testis in vivo and in vitro via upregulating SIRT3. Objective This study evaluates the effects and mechanism of LBSO in the d -galactose ( d -gal)-induced ageing testis. Materials and methods Male Sprague Dawley (SD) rats ( n = 30, 8-week-old) were randomly divided into three groups: LBSO group ( n = 10) where rats received subcutaneous injection of d -gal at 125 mg/kg/day for 8 weeks and intragastric administration of LBSO at 1000 mg/kg/day for 4 weeks, ageing model group ( n = 10) received 8-week-sunbcutaneous injection of d -gal, and control group ( n = 10) with same administration of normal saline. Lentivirus had established TM4 cells with SIRT3 overexpression or silencing before LBSO intervened in vitro . Results Treatment with LBSO, the levels of INHB and testosterone both increased, compared to ageing model. In vitro , we found the ED 50 of LBSO was 86.72 ± 1.49 and when the concentration of LBSO at 100 μg/mL to intervene TM4 cells, the number of cells increased from 8120 ± 676.2 to 15251 ± 1119, and the expression of SIRT3, HO-1, and SOD upregulated. However, HO-1 and SOD were dysregulated by silencing SIRT3. On the other hand, the expression of AMPK and PGC-1 α upregulated as an effect of SIRT3 overexpression by lentivirus, meanwhile the same increasing trend of that being found in cells treated with LBSO, compared to control group. Discussion and conclusions LBSO alleviated oxidative stress in d -gal-induced sub-acutely ageing testis and TM4 cells by suppressing the oxidative stress to mitochondria via SIRT3/AMPK/PGC-1α.

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Section: Introductionmentioning

confidence: 92%

SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

Zhou

Wang

Zhao

et al. 2021

Pharmaceutical Biology

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“…Previous work in our laboratory with mice fed calorie restricted diets showed that the content of both CoQ 9 and CoQ 10 in skeletal muscle were increased in animals fed soybean oil- or fish oil-enriched diets as compared with those fed diets enriched in lard [ 4 , 5 ]. Aged rats fed a diet supplemented with fish oil showed increased CoQ content in heart as compared to animals fed a diet supplemented with sunflower oil [ 15 ]. However, despite the existing relationship between CoQ and dietary fats, the specific mechanism of CoQ regulation by dietary fat remains to be established.…”

Section: Introductionmentioning

confidence: 99%

Regulation of hepatic coenzyme Q biosynthesis by dietary omega-3 polyunsaturated fatty acids

et al. 2021

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Dietary fats are important for human health, yet it is not fully understood how different fats affect various health problems. Although polyunsaturated fatty acids (PUFAs) are generally considered as highly oxidizable, those of the n-3 series can ameliorate the risk of many age-related disorders. Coenzyme Q (CoQ) is both an essential component of the mitochondrial electron transport chain and the only lipid-soluble antioxidant that animal cells can synthesize. Previous work has documented the protective antioxidant properties of CoQ against the autoxidation products of PUFAs. Here, we have explored in vitro and in vivo models to better understand the regulation of CoQ biosynthesis by dietary fats. In mouse liver, PUFAs increased CoQ content, and PUFAs of the n-3 series increased preferentially CoQ 10 . This response was recapitulated in hepatic cells cultured in the presence of lipid emulsions, where we additionally demonstrated a role for n-3 PUFAs as regulators of CoQ biosynthesis via the upregulation of several COQ proteins and farnesyl pyrophosphate levels. In both models, n-3 PUFAs altered the mitochondrial network without changing the overall mitochondrial mass. Furthermore, in cellular systems, n-3 PUFAs favored the synthesis of CoQ 10 over CoQ 9 , thus altering the ratio between CoQ isoforms through a mechanism that involved downregulation of farnesyl diphosphate synthase activity. This effect was recapitulated by both siRNA silencing and by pharmacological inhibition of farnesyl diphosphate synthase with zoledronic acid. We highlight here the ability of n-3 PUFAs to regulate CoQ biosynthesis, CoQ content, and the ratio between its isoforms, which might be relevant to better understand the health benefits associated with this type of fat. Additionally, we identify for the first time zoledronic acid as a drug that inhibits CoQ biosynthesis, which must be also considered with respect to its biological effects on patients.

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“…The sunflower seed oil, containing both anti-inflammatory (oleic acid, vitamin E, antioxidants, etc.) and pro-inflammatory (linoleic acid) components (14)(15)(16)(17)(18)(19)(20), has lower anti-inflammatory properties than fish oil (21). Although the anti-or pro-inflammatory effect of sunflower seed oil is still a debate, accumulating evidence suggests that it has anti-inflammatory properties (14)(15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Preventive Effects of Fish Oil and Sunflower Seed Oil on the Pathophysiology of Sepsis in Endotoxemic Rats

Kuo

Chan

et al. 2022

Front. Nutr.

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Heart Histopathology and Mitochondrial Ultrastructure in Aged Rats Fed for 24 Months on Different Unsaturated Fats (Virgin Olive Oil, Sunflower Oil or Fish Oil) and Affected by Different Longevity

Cited by 14 publications

References 59 publications

SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

Regulation of hepatic coenzyme Q biosynthesis by dietary omega-3 polyunsaturated fatty acids

Evaluation of the Preventive Effects of Fish Oil and Sunflower Seed Oil on the Pathophysiology of Sepsis in Endotoxemic Rats

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