Heat and oxidative stress alter the expression of orexin and its related receptors in avian liver cells

Greene, Elizabeth S.; Khaldi, Stephanie; Ishola, Peter; Bottje, Walter; Ohkubo, Tadayasu; Anthony, Nicholas B.; Dridi, Sami

doi:10.1016/j.cbpa.2015.08.016

Cited by 15 publications

(18 citation statements)

References 49 publications

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“…In contrast to our previous study where we have shown that heat stress down-regulated the expression of orexin at both mRNA and protein levels in quail liver (Greene et al, 2016 ), heat stress dysregulated only orexin protein but not mRNA abundances in quail muscle in the current study. This indicated that heat stress might regulate orexin expression at post-transcriptional and/or translational levels in quail muscle (Sakurai et al, 1998 ; Cai et al, 2000 ; Chen and Randeva, 2010 ).…”

Section: Discussioncontrasting

confidence: 99%

“…Similar to heat stress, H 2 O 2 treatment dysregulated the expression of orexin system in QM7 cells suggesting that the effect of heat stress might be mediated through induction of oxidative stress and formation of reactive oxygen species (ROS) which merits further in depth investigations. Hepatic orexin expression has been previously shown by our group to be altered by H 2 O 2 (Greene et al, 2016 ) and H 2 O 2 /ROS has recently become a crucial player in regulating neuronal response in a substrate-dependent manner (Kuo et al, 2011 ; Drougard et al, 2015 ). As H 2 O 2 /ROS are now known to act in many signaling pathways in different peripheral organs, the induction of phosphorylated AMPKα1/2 at Thr172 site in the current study (data not shown) suggest that heat and oxidative stress might regulate muscle orexin expression via AMPK pathway.…”

Section: Discussionmentioning

confidence: 95%

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Acute Heat Stress Alters the Expression of Orexin System in Quail Muscle

et al. 2017

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Accumulating evidences indicate that the hypothalamic neuropeptide orexins or hypocretins are involved in stress-induced responses in mammals. Recently, we found that orexin is expressed and secreted from avian muscle cells, however its regulation is still unknown. In this study, we investigated the effect of heat and oxidative stress, the most challenging stressors in poultry production, on the expression of orexin system in quail muscle tissues and myoblast cell lines. Four week-old genetically selected susceptible and resistant Japanese quail (Coturnix coturnix Japonica) lines were exposed to acute heat stress (HS, 37°C for 1.5 h) or maintained at thermoneutral conditions (24°C). Quail myoblast (QM7) cell line was exposed to heat stress (45°C) for 0.5, 1, 2, or 4 h. The control cells were maintained at 37°C. The cells were also treated with several doses of hydrogen peroxide (H2O2, 10–200 μM) or 4-Hydroxynonenal (4-HNE, 10–30 μM) as oxidative stress. Untreated cells were used as controls. Acute HS significantly induced the expression of HSP70 and down-regulated orexin system in both quail muscle tissue and QM7 cells. Similarly, H2O2 but not 4-HNE treatment significantly increased HSP70 protein levels and dysregulated the expression of orexin and its related receptors in a dose-dependent manner in QM7 cells. Transient overexpression of HSP70 down-regulated the expression of orexin system in QM7 cells. Taken together, these data indicate that orexin may be a key player in stress response in avian muscle by demonstrating that heat and oxidative stress alter the expression of orexin system in quail muscle. This effect might be mediated through HSP70. Unraveling the upstream regulators and downstream effectors of orexin in avian muscle merits further in depth investigations.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 95%

Acute Heat Stress Alters the Expression of Orexin System in Quail Muscle

et al. 2017

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“…We speculated that this decrease in feeding may be related to the impaired mobility in hens and stress induced by keel fractures. Some researchers have reported that negative emotions, heat, and oxidative stress downregulate the expression of avian orexin and orexin receptors genes, which may inhibite the feeding motivation ( Greene et al., 2016 , Nguyen et al., 2017 ). Likewise, our previous study has found that keel fracture causes stress response and inhibits the gene expression of orexin and its receptors ( Wei et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Keel fracture changed the behavior and reduced the welfare, production performance, and egg quality in laying hens housed individually in furnished cages

Wei

Xin

et al. 2020

Poultry Science

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Keel fracture has adverse effects on welfare, behavior, health, production performance, and egg quality of laying hens. To investigate this, 90 healthy Lohmann white laying hens with normal keel bones at 17 wk of age ( WOA ) were used in this study and housed individually in furnished cages. All hens were marked with fractured keel ( FK ) or normal keel ( NK ) based on the keel bone status through palpation at 5 time-points (22, 27, 32, 37, and 42 WOA). After the palpation, the behavior was observed for 2 consecutive days at each time-point, and the total number of eggs produced, dirty eggs, broken eggs, and feed intake of FK and NK laying hens were recorded at 27–32, 32–37, and 37–42 WOA, respectively. After each behavioral observation, 10 fresh FK hens and 10 NK hens were randomly selected to determinate the welfare and egg quality. The results showed that the incidences of keel fracture increased with the age of laying hens. Compared with NK hens, the sitting and standing behaviors significantly increased ( P < 0.05) while feeding, walking, perching, and jumping behaviors significantly decreased ( P < 0.05) in FK hens. There were no significant changes in drinking, preening, comforting, cage pecking, and nesting behaviors between NK and FK hens ( P > 0.05). During the experiment period, the egg production rate, body weight, daily feed intake, and eggshell strength, thickness, and weight decreased ( P < 0.05) and duration of tonic immobility increased ( P < 0.05) in FK hens compared with those in NK hens. At 27–32 WOA, FK hens had significantly elevated broken egg rate ( P < 0.05). There were no significant differences in the dirty egg rate, egg shape index, protein height, Haugh unit, feather cover score, and toe and foot pad health score ( P > 0.05). Therefore, keel fracture in laying hens caused changes in behavior and reduced the welfare, production performance, feed intake, and eggshell quality.

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“…In fact, Furuse's group have shown that intracerebroventricular (ICV) administration of orexin did not affect feeding behavior in neonate chicks (Furuse et al, 1999 ). Recently, our group made a breakthrough by identifying that orexin is expressed in chicken muscle and liver and it is responsive to oxidative and HS (Lassiter et al, 2015 ; Greene et al, 2016 ). Additionally, emerging evidence from mammalian studies identified orexin as a stress modulator via its interaction with the hypothalamic-pituitary-adrenal (HPA) axis (Bonnavion et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Noni (Morinda citrifolia) Modulates the Hypothalamic Expression of Stress- and Metabolic-Related Genes in Broilers Exposed to Acute Heat Stress

et al. 2017

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Heat stress (HS) adversely affects growth performance and inflicts heavy economic losses to the poultry industry. There is, therefore, a critical need to identify new alternative strategies to alleviate the negative effects induced by HS. The tropic medicinal plant, Morinda citrifolia (Noni), is being used in livestock nutrition, however the literature is limited and conflicting for its impact on growth performance. The present study aimed to determine the effect of Noni on feeding and drinking behavior as well as on the hypothalamic expression of stress- and metabolic-related genes in broiler chickens exposed to acute HS. A total of 480 1 day-old male broiler chicks were randomly assigned to 12 controlled environmental chambers. Birds were subjected to two environmental conditions (TN, 25°C vs. HS, 35°C for 2 h) and fed two diets (control vs. 0.2% Noni) in a 2 × 2 factorial design. Feed intake and core body temperature (BT) were recorded during HS period. Blood was collected and hypothalamic tissues were harvested for target gene and protein analyses. Acute HS-broilers exhibited higher BT (~1°C), spent less time eating with a significant decrease in feed intake, and spent more time drinking along with higher drinking frequency compared to those maintained under TN conditions. Although Noni supplementation did not improve feed intake, it significantly delayed (~30 min) and reduced the BT-induced by HS. At molecular levels and under HS conditions, Noni supplementation down regulated the hypothalamic expression of HSP90 and its related transcription factors HSF1, 2, and 4, increased orexin mRNA levels, and decreased the phosphorylation levels of AMPKα1/2Thr172 and mTORSer2481. Together, these data indicated that Noni supplementation might modulate HS response in broilers through central orexin-AMPK-mTOR pathways.

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Heat and oxidative stress alter the expression of orexin and its related receptors in avian liver cells

Cited by 15 publications

References 49 publications

Acute Heat Stress Alters the Expression of Orexin System in Quail Muscle

Acute Heat Stress Alters the Expression of Orexin System in Quail Muscle

Keel fracture changed the behavior and reduced the welfare, production performance, and egg quality in laying hens housed individually in furnished cages

Noni (Morinda citrifolia) Modulates the Hypothalamic Expression of Stress- and Metabolic-Related Genes in Broilers Exposed to Acute Heat Stress

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