Heat or cold chronic stress affects organ weights and Hsp70 levels in chicken embryos

Givisiez, Patrícia Emília Naves; Silva, M. M. da; Mazzi, Carmen Maria; Ferro, M. I. T.; Ferro, J. A.; Gonzáles, Elisabeth; Macari, Marcos

doi:10.4141/a00-049

Cited by 36 publications

(19 citation statements)

References 32 publications

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“…The observed enlarged hearts for T2 embryos was consistent with reports of Givisiez et al (2001) and Yalçın and Siegel (2003). Whether this has a role in subsequent susceptibility of broilers to ascites requires future studies.…”

Section: Discussionsupporting

confidence: 80%

Manipulation of Incubation Temperatures to Increase Cold Resistance of Broilers:Influence on Embryo Development, Organ Weights, Hormones and Body Composition

et al. 2012

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Eighteen hundred eggs obtained from Ross broiler breeders at 32 and 48 wk age were randomly assigned to two incubation treatments: T1 eggs were incubated at 37.6℃ throughout, while for T2 eggs the incubation temperature was reduced 1℃ for 6 h daily at embryonic ages (EA) 10 to 18. Embryo and organ weights and body composition were measured at EA 14, 19 and day of hatch (DOH). Hatchability and hatching duration, as well as serum triiodothyronine (T 3 ), thyroxin (T 4 ), and triglycerides were measured at DOH. T1 eggs contained less water than T2 eggs at EA 18. Hatchability was lower and the incubation period was 4.2 h longer for T2 than T1 chicks. On DOH for older breeders chick weights and serum T 3 levels were higher for T2 than T1; however, those from younger breeders were similar at both incubation temperatures. These results may show a beneficial effect of T2 treatment in older breeders. Incubation temperature did not affect triglycerides levels. On DOH, higher body lipids content of T2 than T1 chicks may contribute to their resistance to cold post hatch.

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Section: Discussionsupporting

confidence: 80%

Manipulation of Incubation Temperatures to Increase Cold Resistance of Broilers:Influence on Embryo Development, Organ Weights, Hormones and Body Composition

et al. 2012

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“…A positive correlation was seen between the change in cloacal temperature and Hsp70 levels in the liver of naked neck birds at five days of age (Dionello, 1998). Hsp70 expression was also shown to be tissue-dependent when the levels of this protein were studied in different organs of chicken embryos subjected to heat stress (Givisiez et al, 2001). Naked neck birds, from a breed selected for weight gain (PP1), showed different Hsp70 levels in hepatic cells when submitted to increasing gradual heat stress (28-36°C).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds

Mazzi

Ferro

et al. 2003

Genet. Mol. Biol.

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The promoter region and the beginning of the coding region of the hsp70 stress gene were analysed in broiler chickens of a commercial breed (Hubbard-Pettersen), a breed selected for weight gain (PP1) and a non-selected breed (naked-neck Label Rouge). The naked neck gene (Naked neck, Na), which reduces feathering in birds and is thus related to heat resistance, was present in both PP1 and Label Rouge breeds. Genomic DNA was restricted with PstI and Southern blotting analysis of the samples revealed the absence of polymorphic sites for that enzyme in the promoter region and beginning of the coding region of the hsp70 gene of studied birds. PCR-SSCP analysis of these regions, however, indicated the presence of polymorphisms in the beginning of the coding region and the sequencing of the PCR products confirmed and identified two polymorphic sites in this region: a transition A → G in position +258 and a transversion C → G in position +276. Both mutations were considered to be silent, since they did not modify the aminoacid sequence of the protein Hsp70. The promoter region of the hsp70 gene was identical in all studied birds, indicating that the regulation pattern of this gene must be the same in all birds at the promoter region. Three different alleles (hsp70-1, hsp70-2 and hsp70-3) were identified for the hsp70 gene from the observed mutations. The allele hsp70-3 was detected in only two breeds, Hubbard-Pettersen and PP1, but at a low frequency (0,016 and 0,006, respectively).

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“…Hsps are major molecular chaperones that perform important functions in the folding/unfolding and translocation of proteins as well as in the assembly/disassembly of protein complexes (Bernabo et al 2011). Meanwhile, Hsps are also expressed constitutively in poultry species (Givisiez et al 2001). Among HSP families, Hsp70 is the most extensively studied because of its prominent response to diverse stressors, and increased synthesis of these inducible proteins is involved in the protection of stressed cells and organisms (Gabriel et al 2002).…”

Section: Introductionmentioning

confidence: 99%

The role of heat shock proteins in inflammatory injury induced by cold stress in chicken hearts

Zhao

Zhang

Wang

et al. 2013

Cell Stress and Chaperones

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The aim of this study was to investigate the effects of cold stress on the expression levels of heat shock proteins (Hsps90, 70, 60, 40, and 27) and inflammatory factors (iNOS, COX-2, NF-κB, TNF-α, and PTGEs) and oxidative indexes in hearts of chickens. Two hundred forty 15-day-old male chickens were randomly divided into 12 groups and kept at the temperature of 12±1°C for acute and chronic cold stress. There were one control group and five treatment groups for acute cold stress, three control groups, and three treatment groups for chronic cold stress. After cold stress, malondialdehyde level increased in chicken heart; the activity of superoxide dismutase and glutathione peroxidase in the heart first increased and then decreased. The inflammatory factors mRNA levels were increased in cold stress groups relative to control groups. The histopathological analysis showed that heart tissues were seriously injured in the cold stress group. Additionally, the mRNA levels of Hsps (70, 60, 40, and 27) increased significantly (P<0.05) in the cold stress groups relative to the corresponding control group. Meanwhile, the mRNA level and protein expression of Hsp90 decreased significantly (P<0.05) in the stress group, and showed a gradually decreasing tendency. These results suggested that the levels of inflammatory factors and Hsps expression levels in heart tissues can be influenced by cold stress. Hsps commonly played an important role in the protection of the heart after cold stress.

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Heat or cold chronic stress affects organ weights and Hsp70 levels in chicken embryos

Cited by 36 publications

References 32 publications

Manipulation of Incubation Temperatures to Increase Cold Resistance of Broilers:Influence on Embryo Development, Organ Weights, Hormones and Body Composition

Manipulation of Incubation Temperatures to Increase Cold Resistance of Broilers:Influence on Embryo Development, Organ Weights, Hormones and Body Composition

Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds

The role of heat shock proteins in inflammatory injury induced by cold stress in chicken hearts

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