Heat shock protein 60 negatively regulates the biological functions of ubiquitin-like protein MNSFβ in macrophages

Communications Between Bone Marrow Macrophages and Bone Cells in Bone Remodeling

“…NF-kB, composed of subunits such as p65, is a downstream transcription factor of the RANKL-RANKL signaling pathway (Park et al, Interfere the PTEN/PI3K/AKT signaling pathway to regulate the biological behavior of cells Zheng et al, 2017;Yang et al, 2018 mir-155 Promote the secretion of inflammatory cytokines Stanczyk et al, 2008;Heymans et al, 2013;Ying et al, 2017 mir-5106 Inhibit the genes SIK2 and SIK3 to promote bone formation Xiong et al, 2020 Alarmins Annexins Induce bone resorption and help macrophages to work Li et al, 2005;D'Souza et al, 2012;Stukes et al, 2016;McArthur et al, 2020;Xia et al, 2020;Xiong et al, 2020 Galectins Act on the differentiation of various bone cells Andersen et al, 2003;Shimura et al, 2005;Nakajima et al, 2014;Weilner et al, 2016;Simon et al, 2017;Iacobini et al, 2018 HSP; fibronectin Functions through osteoblasts and osteoclasts. Moursi et al, 1996;Koh et al, 2009;Gramoun et al, 2010;Notsu et al, 2016;Nakamura et al, 2019 2017). Nuclear factor of activated T cells cytoplasmic 1 (NFATc1) is a master regulator of osteoclast differentiation (Okamoto and Takayanagi, 2011).…”

“…Galectins mainly affect the differentiation of bone marrow stromal cells (Andersen et al, 2003), osteoblast, osteoclast (Shimura et al, 2005;Nakajima et al, 2014;Simon et al, 2017;Iacobini et al, 2018) and the osteogenesis of mesenchymal stem cells (Weilner et al, 2016). What's more, HSP-60 (Koh et al, 2009), HSP-70 (Notsu et al, 2016;Nakamura et al, 2019) and fibronectin are also primarily concerned with osteoblasts (Moursi et al, 1996) and osteoclasts (Gramoun et al, 2010) to come into play ( Table 1).…”

MNSFβ Regulates TNFα Production by Interacting with RC3H1 in Human Macrophages, and Dysfunction of MNSFβ in Decidual Macrophages Is Associated With Recurrent Pregnancy Loss

“…We hypothesized that MNSFβ might regulate the production of TNFα by different molecular pathways in different cells. Although several molecules, such as Bcl-G ( 10 ), endocytosin II ( 11 ), HSPA8 ( 33 ), and Hsp60 ( 34 ), have been identified to bind with MNSFβ in murine Mϕ, none of them could explain the promoting effect of MNSFβ on promoting TNFα expression. Thus, we identified candidates in the BioGRID database ( Supplementary Figure S3 ) and found that MNSFβ potentially binds to RC3H1, which could inhibit the expression of TNFα by degrading its mRNA ( 24 , 35 ), and as an immune regulator, RC3H1 is involved in T-cell activation ( 36 ).…”

MNSFβ regulates placental development by conjugating IGF2BP2 to enhance trophoblast cell invasiveness

“…However, it is notable that among the candidates that potentially bind to MNSFβ, ribosomal protein S3A (RPS3A) was reported to promote the biological processes related to tumourigenesis, metastasis and immunosuppression in hepatocellular carcinoma patients, 31 in formate dehydrogenase (FDH). 30,[33][34][35] K139 in IGF2BP2 has been the predominant site for its ubiquitination. 36 We thus assume that the covalent binding between G74 in MNSFβ with K139 in IGF2BP2 may be the recognition site to protect the degradation of IGF2BP2.…”