Heat shock protein 70 membrane expression on fibroblast‐like synovial cells derived from synovial tissue of patients with rheumatoid and juvenile idiopathic arthritis

Nguyen, Thị Thu Hien; Gehrmann, Mathias; Zlacka, Denisa; Sosna, A; Vavrincová, P; Multhoff, Gabriele; Hromadníková, Ilona

doi:10.1080/03009740600759621

Cited by 19 publications

(11 citation statements)

References 19 publications

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“…Pannus tissue adheres to the surface of articular cartilage, and proliferating FLS within the pannus produce proteinases that degrade and destroy the cartilage and underlying cortical bone (31). A fully formed pannus is characterized by: proliferation of FLS; inflammatory infiltrates comprised predominantly of lymphocytes, plasma cells, and macrophages; and marked angiogenesis (32)(33)(34)(35). NGF and its receptor system are known to influence several of these crucial biological events associated with the formation of pannus in inflammatory arthritis, for example angiogenesis (19), activation of T cells (36), upregulation of chemokines (37) and neuropeptides (38), induction of nerve proliferation (22), and regulation of pain control (39,40).…”

Section: Discussionmentioning

confidence: 99%

The regulatory role of nerve growth factor and its receptor system in fibroblast‐like synovial cells

Raychaudhuri¹,

Raychaudhuri

2009

Scandinavian Journal of Rheumatology

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Upregulation of NGF/TrkA in proinflammatory cytokine-activated FLS, the mitogenic effect of NGF on FLS, and the increased NGF level in SF of inflammatory arthritis suggest that there is cross-talk between NGF/NGF-R and FLS. These results also suggest that dysregulated production of NGF may lead to synovial cell proliferation and thus could influence the inflammatory and proliferative cascades of inflammatory arthritis.

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Section: Discussionmentioning

confidence: 99%

The regulatory role of nerve growth factor and its receptor system in fibroblast‐like synovial cells

Raychaudhuri¹,

Raychaudhuri

2009

Scandinavian Journal of Rheumatology

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“…Alternatively, ligation of HSP70 and autoantigens may promote autoantigen processing and presentation. (55)…”

Section: Pathogenesismentioning

confidence: 99%

Oligoarticular and polyarticular JIA: epidemiology and pathogenesis

et al. 2009

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Summary Juvenile idiopathic arthritis (JIA) refers to a group of chronic childhood arthropathies, currently classified into subtypes primarily on the basis of clinical features. Research has focused on the hypothesis that these subtypes arise through distinct etiologic pathways. In this Review, we discuss four subtypes of JIA: persistent oligoarticular, extended oligoarticular, rheumatoid-factor positive polyarticular and rheumatoid-factor-negative polyarticular. These subtypes differ in prevalence between ethnic groups and are associated with different HLA alleles. Non-HLA genetic risk factors have also been identified, some of which reveal further molecular differences between these subtypes, while others suggest mechanistic overlap. Investigations of immunophenotypes also provide insights into subtype differences: adaptive immunity appears to have a prominent role in both polyarticular and oligoarticular JIA, and the more-limited arthritis observed in persistent oligoarticular JIA as compared with extended oligoarticular JIA may reflect more-potent immunoregulatory T-cell activity in the former. Tumor necrosis factor seems to be a key mediator of both polyarticular and oligoarticular JIA, especially in the extended oligoarticular subtype, although elevated levels of other cytokines also are observed. Limited data on monocytes, dendritic cells, B cells, natural killer T cells, and neutrophils suggest that the contributions of these cells differ across subtypes of JIA. Within each subtype, however, common pathways appear to drive joint damage.

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“…With regard to unprocessed biological samples, Hsp70 cell surface expression was negative in most cases, except in two patients with de novo AML, where Hsp70 positive blasts, showing rather more mature features, were identified in fresh peripheral blood and bone marrow samples. Interestingly, we repeatedly observed that peripheral blood monocytes, derived either from healthy controls or patients with rheumatoid arthritis, were frequently Hsp70 positive, apparently due to the binding of extracellular Hsp70 to Hsp receptors such as CD14, CD91, and Toll-like receptors expressed on the surface of monocytes [30].…”

Section: Discussionmentioning

confidence: 98%

“…A cut-off value for Hsp70, of 10%, was chosen based on results from previous screenings of normal peripheral blood lymphocytes using flow cytometry [30,31].…”

Section: Healthy Controlsmentioning

confidence: 99%

Heat-shock protein expression in leukemia

et al. 2010

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Heat-shock proteins (Hsps) are thought to play a role in the development of cancer and to modulate tumor response to cytotoxic therapy. In this study, Hsp27, Hsp60, Hsp90α, and HspBP1 gene expression was investigated in human leukemia cell lines as well as in leukemia cells derived from patients with the onset of the disease. Hsp70 membrane expression and expression of Hsp27, Hsp60, Hsp70, Hsp90α, and HspBP1 genes were also tested in samples from leukemia patients. Relative Hsps gene expression was examined in human leukemia cell lines and also in patients, using real-time quantitative reverse-transcriptase polymerase chain reaction (RT-PCR). Hsp70 cell surface expression was studied in patients with leukemia onset using flow cytometry. All tested cell lines showed significantly increased expression of Hsp60, Hsp90α, and HspBP1 genes compared with a cohort of healthy controls; additionally there was increased Hsp27 expression except for Jurkat and CCRF cells. Significantly higher gene expression of Hsp27, Hsp60, Hsp90α, and HspBP1 was observed in the peripheral blood of patients compared with bone marrow and healthy control samples, while Hsp70 expression was without any significant difference among cohorts. Hsp70 cell surface expression was found on defrosted and cultured leukemia cells but not on unprocessed biological samples from patients. Leukemia cells showed a heterogeneous pattern of Hsp gene expression among leukemia cell lines as well as in peripheral blood and bone marrow of patients.

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Heat shock protein 70 membrane expression on fibroblast‐like synovial cells derived from synovial tissue of patients with rheumatoid and juvenile idiopathic arthritis

Abstract: Fibroblast-like synovial cells derived from patients with severe course of RA and JIA are strongly positive for membrane-expressed Hsp70.

Cited by 19 publications

References 19 publications

The regulatory role of nerve growth factor and its receptor system in fibroblast‐like synovial cells

The regulatory role of nerve growth factor and its receptor system in fibroblast‐like synovial cells

Oligoarticular and polyarticular JIA: epidemiology and pathogenesis

Heat-shock protein expression in leukemia

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