Heat stress effect on the intestinal epithelial function of broilers fed methionine supplementation

Vesco, Ana Paula Del; Khatlab, Angélica de Souza; Santana, Thaís Pacheco; Pozza, Paulo César; Soares, Maria Amélia Menck; Brito, Cláudson Oliveira; Barbosa, Leandro Teixeira; Gasparino, Eliane

doi:10.1016/j.livsci.2020.104152

Cited by 15 publications

(4 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Evidence is growing that the HSP70 interacts with inflammatory cytokines to diminish inflammation by boosting the immunity of the small intestine ( Borges et al, 2012 ). In the present research, there was overexpression in HSP70 mRNA under HS conditions which is consonant with the former studies ( Vesco et al, 2020 ; Siddiqui et al, 2020 ). Comparable to Li et al (2019) study, BT treatment alleviated heat-induced increased HSP70 mRNA expression.…”

Section: Discussionsupporting

confidence: 93%

Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress

et al. 2021

View full text Add to dashboard Cite

This research was executed to study the impacts of adding betaine ( BT ) to broiler diets on intestinal inflammatory response and barrier integrity under heat stress ( HS ). At 21 d of age, 150 male broilers (Ross 308) were randomly assigned to 3 treatment groups: control ( CON ) group, in which broilers were provided standard finisher feed under thermoneutral condition (22 ± 1°C); HS group and HS + BT group, in which broilers were given the standard feed supplied with 0 and 1,000 mg/kg BT, respectively, under cyclic HS condition (33 ± 1°C for 8 h from 08:00 to 16:00 h and the thermoneutral temperature for the residual hours). Each treatment was replicated ten times with 5 broilers per replicate. The HS group showed an elevation ( P < 0.05) in serum corticosterone ( CORT ) concentration, D-lactate acid ( D-LA ) content, and diamine oxidase ( DAO ) activity, mucosal interleukin-1β ( IL-1β ) level, and expression of heat shock protein 70 ( HSP70 ) gene, and a reduction ( P < 0.05) in mucosal interleukin-10 ( IL-10 ) level and secretory immunoglobulin A ( SIgA ) content and relative abundance of mRNA for occludin ( OCLN ), zonula occludens-1 ( ZO-1 ), claudin-1 ( CLDN1 ), and claudin-4 ( CLDN4 ). In contrast, broilers in the HS + BT group exhibited a raise ( P < 0.05) in mucosal IL-10 level and SIgA content and relative expression of OCLN and ZO-1 genes, and a decline ( P < 0.05) in serum CORT concentration and DAO activity, mucosal IL-1β level, and expression of HSP70 mRNA. These results indicate that supplemental BT can ameliorate intestinal injury in heat-challenged broilers by suppressing inflammatory responses and enhancing mucosal barrier function.

show abstract

Section: Discussionsupporting

confidence: 93%

Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Corresponding with our findings, it was found that thermal exposure increases the expression of CLDN1 [14][15][16], OCLN [16], CDH1, and CLDN5 [14]. These findings suggest that upregulation in gene expression is a compensatory response to the damaged junctional proteins or to the rupture of the intestinal epithelia.…”

Section: Impact Of Tml and Tmh Temperatures On The Expression Of Junctional And Heat Shock Genes In Broilers Subjected To Thermal Stresssupporting

confidence: 89%

“…Any damage to junctional proteins increases the permeability of the intestinal barrier to luminal antigens and may facilitate the translocation of pathogens across it, resulting in endogenous infection and eventual endotoxemia that impairs the absorption of nutrients [11,12]. The previous studies had reported that thermal stress could affect intestinal integrity by modulating the expression of junction and heat shock proteins in the small intestine of broiler chickens and subsequently increasing the expression of pro-inflammatory cytokines and toll-like receptors (TLR) [13][14][15][16][17][18][19][20]. In addition, thermal stress was reported to have severe effect on a broiler's nutrient absorption capability by modifying the intestine's histomorphometric parameters, leading to intestinal tissue injury [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Molecular and morphometric changes in the small intestine during hot and cold exposure in thermally manipulated broiler chickens

2021

View full text Add to dashboard Cite

Background and Aim: Thermal stress (hot or cold) is one of many environmental stressors that severely affects the health of broiler chickens. One negative effect of thermal stress is the disruption of the intestinal barrier function in broiler chickens. This study aimed to evaluate the effect of thermal manipulation (TM) on the small intestine in terms of histomorphometry as well as junctional, heat-shock, and immune response gene expression during post-hatch exposure to thermal stress. Materials and Methods: The experiment was conducted by dividing 928 fertile Ross eggs into three incubation groups: The control (C) group (incubated at 37.8°C and 56% relative humidity [RH] for the whole incubation period), the TM using low temperature TML group (incubated at 36°C and 56% RH for 18 h/day from embryonic days 7 to 16), and the TM using high temperature (TMH) group (incubated at 39°C and 65% RH for 18 h/day from embryonic days 7 to 16). On post-hatch day 21, 90 chicks were randomly selected from each incubation group and were equally subdivided into three subgroups for the post-hatch thermal stress experiment: The TN subgroup (room temperature maintained at 24°C), the heat stress (HS) subgroup (room temperature maintained at 35°C), and the cold stress (CS) subgroup (room temperature maintained at 16°C). After 1 day of thermal stress exposure (age 22 days), five birds from each subgroup were euthanized and ileum samples were collected to evaluate the transcription of the Claudin (CLDN1), CLDN-5, Occludin, Cadherin-1, heat shock factors (HSF1), HSF3, 70 kilodalton heat shock protein, 90 kilodalton heat shock protein, Interleukin 6 (IL6), IL8, toll-like receptors-2 (TLR2), and TLR4 genes by Real-Time Quantitative Reverse Transcription polymerase chain reaction analysis. Finally, after 4 and 7 days of thermal stress (age 25 and 28 days, respectively), nine chicks were euthanized, and their jejunum and ileum were collected for histomorphometric analysis. Results: After exposure to 1 day of thermal stress, the C subgroups exposed to thermal stress (HS and CS) possessed significantly increased expression of junctional, heat-shock, and immune response genes compared to the C-TN subgroup, and similar results were observed for the TMH. In contrast, thermally stressed TMH subgroups had significantly lower expression of the studied genes compared to C subgroups exposed to thermal stress. Furthermore, no significant changes were detected between the TML subgroups exposed to thermal stress and TML-TN. Moreover, significant alterations in villus height (VH), villus surface area, crypt depth (CD), and VH to CD ratio were observed between the TML, TMH, and C subgroups exposed to CS. Conclusion: It might be suggested that TM may have a protective impact on the small intestine histomorphometry and epithelial integrity of broilers during post-hatch exposure to thermal stress.

show abstract

“…Dietary Met supplementation in broilers improved the GSH:GSSG ratio and glutathione peroxidase activity as well as increased the villus height (VH) and ratio of villus height to crypt depth (VH:CD) with or without stocking density challenge [ 29 ]. Met supplementation also improved the tight junction protein expression, whereas it decreased the expression of proinflammatory cytokines in broilers under heat stress [ 30 ]. Another study also demonstrated that Met supplementation increased the concentration of GSH, while it reduced the malondialdehyde (MDA) content in the duodenum mucosa.…”

Section: Methionine and Arginine In Intestinal Healthmentioning

confidence: 99%

The Functional Roles of Methionine and Arginine in Intestinal and Bone Health of Poultry: Review

Liu,

Kim

2023

Animals

View full text Add to dashboard Cite

This review explores the roles of methionine and arginine in promoting the well-being of poultry, with a specific focus on their impacts on intestinal and bone health. The metabolic pathways of methionine and arginine are elucidated, highlighting their distinct routes within the avian system. Beyond their fundamental importance in protein synthesis, methionine and arginine also exert their functional roles through their antioxidant capacities, immunomodulating effects, and involvement in the synthesis of metabolically important molecules such as S-adenosylmethionine, nitric oxide, and polyamines. These multifaceted actions enable methionine and arginine to influence various aspects of intestinal health such as maintaining the integrity of the intestinal barrier, regulating immune responses, and even influencing the composition of the gut microbiota. Additionally, they could play a pivotal role in promoting bone development and regulating bone remodeling, ultimately fostering optimal bone health. In conclusion, this review provides a comprehensive understanding of the potential roles of methionine and arginine in intestinal and bone health in poultry, thereby contributing to advancing the nutrition, overall health, and productivity of poultry in a sustainable manner.

show abstract

Heat stress effect on the intestinal epithelial function of broilers fed methionine supplementation

Cited by 15 publications

References 49 publications

Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress

Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress

Molecular and morphometric changes in the small intestine during hot and cold exposure in thermally manipulated broiler chickens

The Functional Roles of Methionine and Arginine in Intestinal and Bone Health of Poultry: Review

Contact Info

Product

Resources

About