Heat Stress Induces an Aggregation of the Light-Harvesting Complex of Photosystem II in Spinach Plants

Tang, Yun; Wen, Xiaopeng; Lu, Qingtao; Yang, Zhipan; Cheng, Zhukuan; Lu, Congming

doi:10.1104/pp.106.090712

Cited by 125 publications

(75 citation statements)

References 54 publications

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“…These are (a) protein-protein interactions between neighboring antenna complexes in the grana macrostructure (4,30), sometimes under stress conditions, forming large aggregates in which deeply quenched states may be stabilized (31,32); (b) zeaxanthin, critical for the full expression of NPQ in vivo, allosterically favoring the quenched conformation of LHCII (4,33); and (c) the ⌬pH via the action of the PsbS protein acting as the main physiological trigger of the switch (34).…”

Section: Discussionmentioning

confidence: 99%

Induction of Efficient Energy Dissipation in the Isolated Light-harvesting Complex of Photosystem II in the Absence of Protein Aggregation

Ilioaia

Johnson

Horton

et al. 2008

Journal of Biological Chemistry

105

108

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Under excess illumination, the Photosystem II light-harvesting antenna of higher plants has the ability to switch into an efficient photoprotective mode, allowing safe dissipation of excitation energy into heat. In this study, we show induction of the energy dissipation state, monitored by chlorophyll fluorescence quenching, in the isolated major light-harvesting complex (LHCII) incorporated into a solid gel system. Removal of detergent caused strong fluorescence quenching, which was totally reversible. Singlet-singlet annihilation and gel electrophoresis experiments suggested that the quenched complexes were in the trimeric not aggregated state. Both the formation and recovery of this quenching state were inhibited by a cross-linker, implying involvement of conformational changes. Absorption and CD measurements performed on the samples in the quenched state revealed specific alterations in the spectral bands assigned to the red forms of chlorophyll a, neoxanthin, and lutein 1 molecules. The majority of these alterations were similar to those observed during LHCII aggregation. This suggests that not the aggregation process as such but rather an intrinsic conformational transition in the complex is responsible for establishment of quenching. 77 K fluorescence measurements showed redshifted chlorophyll a fluorescence in the 690 -705 nm region, previously observed in aggregated LHCII. The fact that all spectral changes associated with the dissipative mode observed in the gel were different from those of the partially denatured complex strongly argues against the involvement of protein denaturation in the observed quenching. The implications of these findings for proposed mechanisms of energy dissipation in the Photosystem II antenna are discussed.

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Section: Discussionmentioning

confidence: 99%

Induction of Efficient Energy Dissipation in the Isolated Light-harvesting Complex of Photosystem II in the Absence of Protein Aggregation

Ilioaia

Johnson

Horton

et al. 2008

Journal of Biological Chemistry

105

108

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“…In many food processes the temperature exceeds 35°C, which can lead to an irreversible aggregation 34 . The thylakoid's ability to reduce pancreatic lipase activity, e.g.…”

mentioning

confidence: 99%

The effect of heat treatment of thylakoids on their ability to inhibit in vitro lipase/co-lipase activity

et al. 2014

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The effect of heat treatment of thylakoids on their ability to inhibit in vitro lipase/colipase activity.Östbring, Karolina; Rayner, Marilyn; Sjöholm, Ingegerd; Otterström, Jennie; Albertsson, PerÅke; Emek, Sinan Cem; Erlanson-Albertsson, Charlotte General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal AbstractThylakoids has been shown to prolong lipolysis by the inhibition of lipase/co-lipase, which makes thylakoids suitable as a functional food ingredient with satiating properties. The components of thylakoids that provide its function as a lipolysis modulator are primarily photosystems I and II, which are structurally stabilised by chlorophyll. However, chlorophyll is known to be heat sensitive yet the enzymatic inhibiting capacity after heat treatment has not been previously studied. It was hypothesised that the retained function of thylakoids after heat treatment could be correlated to the degree of degradation. Heat treatment at either 60°C, 75°C or 90°C for time interval ranging from 15 sec to 120 min induced a color shift from bright green to olive brown which was attributed to degradation. The ability of heat-treated thylakoids to inhibit lipolysis in vitro was also reduced. A correlation between chlorophyll a degradation and the enzymatic inhibiting capacity could be established which opens possibilities to use a spectrophotometric method to quantify the ability of thylakoids to inhibit lipase/co-lipase in a more rapid and cost effective way to complement the pH-stat method used today. With the degradation pattern investigated, it is then possible to design a thermal treatment process to ensure a microbiological safe appetite-reducing product and at the same time minimize the loss of functionality.

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“…The F m decline might be related to aggregation of the light-harvesting complexes of PSII, as observed by Tang et al (2007) in heat-stressed spinach (Spinacia oleracea L.). Alternatively, a reduction in F m may result from initial inactivation of the oxygen evolving complex (Lu and Zhang 2000).…”

Section: Discussionmentioning

confidence: 99%

Light-stimulated heat tolerance in leaves of two neotropical tree species, Ficus insipida and Calophyllum longifolium

Krause

Winter

Krause

et al. 2015

Functional Plant Biol.

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Abstract. Previous heat tolerance tests of higher plants have been mostly performed with darkened leaves. However, under natural conditions, high leaf temperatures usually occur during periods of high solar radiation. In this study, we demonstrate small but significant increases in the heat tolerance of illuminated leaves. Leaf disks of mature sun leaves from two neotropical tree species, Ficus insipida Willd. and Calophyllum longifolium Willd., were subjected to 15 min of heat treatment in the light (500 mmol photons m -2 s -1 ) and in the dark. Tissue temperatures were controlled by floating the disks on the surface of a water bath. PSII activity was determined 24 h and 48 h after heating using chlorophyll a fluorescence. Permanent tissue damage was assessed visually during long-term storage of leaf sections under dim light. In comparison to heat treatments in the dark, the critical temperature (T 50 ) causing a 50% decline of the fluorescence ratio F v /F m was increased by~1 C (from~52.5 C to~53.5 C) in the light. Moreover, illumination reduced the decline of F v /F m as temperatures approached T 50 . Visible tissue damage was reduced following heat treatment in the light. Experiments with attached leaves of seedlings exposed to increasing temperatures in a gas exchange cuvette also showed a positive effect of light on heat tolerance.

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Heat Stress Induces an Aggregation of the Light-Harvesting Complex of Photosystem II in Spinach Plants

Cited by 125 publications

References 54 publications

Induction of Efficient Energy Dissipation in the Isolated Light-harvesting Complex of Photosystem II in the Absence of Protein Aggregation

Induction of Efficient Energy Dissipation in the Isolated Light-harvesting Complex of Photosystem II in the Absence of Protein Aggregation

The effect of heat treatment of thylakoids on their ability to inhibit in vitro lipase/co-lipase activity

Light-stimulated heat tolerance in leaves of two neotropical tree species, Ficus insipida and Calophyllum longifolium

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