Heating effects on NG108 cells induced by laser trapping

Vilanova, Ione Verdeny; Fontaine, Arnold; Farré, Arnau; Montes-Usategui, Mario; Martı́n-Badosa, Estela

doi:10.1117/12.893352

Cited by 3 publications

(2 citation statements)

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“…12 There are several methods to create periodic multiple-traps, each with its own advantages and disadvantages. 13 One of the most popular and powerful methods to create a periodic potential, an optical lattice, is the use of the interference effects of multiple coherent beams. 14 One important advantage of using interference methods to produce an optical lattice is that the geometry of the lattice is under complete control.…”

Section: Introductionmentioning

confidence: 99%

Light-driven nanomotors with reciprocating motion and high controllability based on interference techniques

Mohammadnezhad,

Saeed,

Abdulkareem

et al. 2024

Nanoscale Adv.

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Section: Introductionmentioning

confidence: 99%

Light-driven nanomotors with reciprocating motion and high controllability based on interference techniques

Mohammadnezhad,

Saeed,

Abdulkareem

et al. 2024

Nanoscale Adv.

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“…23) Photon absorption is a major detrimental process when laser radiation interacts with cells, consequently, generating thermodamage. [23][24][25][26][27][28][29] A temperature rise can lead to the activation of a stress response or heat-shock response in the trapped cells, and cell mechanisms and physiological functions start to fail when the temperature exceeds the damage threshold. Therefore, the photothermal effect and photodamage on the trapped cells will hinder long-time manipulation and measurement of individual cells.…”

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confidence: 99%

Reducing photodamage in optical trapping of individual cells in living zebrafish

Liu

Zhao

Wang

et al. 2020

Appl. Phys. Express

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We propose to reduce the photodamage in optical trapping of living cells by using azimuthally polarized beams (APBs). APBs achieve higher axial trapping efficiency and lower photodamage than linearly polarized Gaussian beams when used for optical trapping of individual red blood cells (RBCs). In particular, we further achieve reducing photodamage in optical trapping of individual RBCs in living zebrafish. Our work is expected to benefit in vivo optical trapping and study of living cells.

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Optimized back-focal-plane interferometry directly measures forces of optically trapped particles

2012

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Back-focal-plane interferometry is used to measure displacements of optically trapped samples with very high spatial and temporal resolution. However, the technique is closely related to a method that measures the rate of change in light momentum. It has long been known that displacements of the interference pattern at the back focal plane may be used to track the optical force directly, provided that a considerable fraction of the light is effectively monitored. Nonetheless, the practical application of this idea has been limited to counter-propagating, low-aperture beams where the accurate momentum measurements are possible. Here, we experimentally show that the connection can be extended to single-beam optical traps. In particular, we show that, in a gradient trap, the calibration product κ·β (where κ is the trap stiffness and 1/β is the position sensitivity) corresponds to the factor that converts detector signals into momentum changes; this factor is uniquely determined by three construction features of the detection instrument and does not depend, therefore, on the specific conditions of the experiment. Then, we find that force measurements obtained from back-focal-plane displacements are in practice not restricted to a linear relationship with position and hence they can be extended outside that regime. Finally, and more importantly, we show that these properties are still recognizable even when the system is not fully optimized for light collection. These results should enable a more general use of back-focal-plane interferometry whenever the ultimate goal is the measurement of the forces exerted by an optical trap.

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Heating effects on NG108 cells induced by laser trapping

Cited by 3 publications

References 0 publications

Light-driven nanomotors with reciprocating motion and high controllability based on interference techniques

Light-driven nanomotors with reciprocating motion and high controllability based on interference techniques

Reducing photodamage in optical trapping of individual cells in living zebrafish

Optimized back-focal-plane interferometry directly measures forces of optically trapped particles

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