Heavy metal mediated immunomodulation of the Indian green frog, Euphlyctis hexadactylus (Anura:Ranidae) in urban wetlands

Priyadarshani, S.; Madhushani, W.A.N.; Jayawardena, Uthpala A.; Wickramasinghe, Deepthi; Udagama, Preethi

doi:10.1016/j.ecoenv.2015.02.037

Cited by 36 publications

(11 citation statements)

References 61 publications

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“…The present study was undertaken to evaluate biochemical and histopathological alterations of a common anuran amphibian, E. hexadactylus , with natural and laboratory exposure to heavy metals, compared with their counterparts collected from a pristine reference site. The study revealed that the Bellanwila‐Attidiya Sanctuary is highly contaminated with various heavy metal species, at concentrations varying on the order of Cu > Cr > Pb > Zn > Cd, and significant bioaccumulation of Cu and Cr in frog liver and gastrocnemius muscle tissue, confirming previous observations . Histopathology of all the major frog organs revealed tissue disturbances as significant alterations from the general morphology observed in the reference animals.…”

Section: Discussionsupporting

confidence: 84%

Heavy metal–induced toxicity in the Indian green frog: Biochemical and histopathological alterations

Jayawardena

Angunawela

Wickramasinghe

et al. 2017

Enviro Toxic and Chemistry

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Heavy metal contamination may have adverse effects on wetland biota, particularly on amphibians. Severe immunotoxic effects elicited in Euphlyctis hexadactylus (Indian green frog) because of metal exposure (Cd, Cr, Cu, Pb, and Zn) in the Bellanwila-Attidiya Sanctuary, a polluted urban wetland in Sri Lanka, provided the rationale for the present study. We evaluated the biochemical and histopathological effects of this metal contamination with a reference E. hexadactylus population and a laboratory exposure group that was subjected to 28 d of exposure to a mixture of Cd, Cr, Cu, Pb, and Zn (5 ppm in each mixture). A histopathological scoring for the semiquantification of tissue damage was established. Results of the biochemical and histopathological markers were remarkably consistent between the 2 exposure scenarios, providing validation for the heavy metal exposure hypothesis. Damage to liver, kidney, lung, and skin of metal-exposed E. hexadactylus quantified multiple impairments absent in the reference frogs. Liver injuries complemented significantly elevated aspartate transaminase (AST), alanine transaminase (ALT), γ-glutamyl transferase (γ-GT), and alkaline phosphatases in frog liver homogenate, indicating hepatocellular leakage and loss of functional and structural integrity of the hepatocyte membrane in both field- and laboratory-exposed frogs. Significant elevation of Kupffer cell hypertrophy, pigmentation, inflammatory cell infiltrates and hepatic inflammation, extramedullary hematopoiesis, karyocytomegaly of hepatocytes (p < 0.05) of the liver, and degeneration of epithelia and necrosis of the lung, manifested as impairments in both metal exposure scenarios. Significantly reduced serum total protein and albumin and significantly elevated urea and creatinine in metal-exposed frogs were indicative of hepatic and renal dysfunction, respectively. The present study affirms histopathology-related biochemical alterations as potential biomarkers for heavy metal toxicity in amphibians. Environ Toxicol Chem 2017;36:2855-2867. © 2017 SETAC.

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Section: Discussionsupporting

confidence: 84%

Heavy metal–induced toxicity in the Indian green frog: Biochemical and histopathological alterations

Jayawardena

Angunawela

Wickramasinghe

et al. 2017

Enviro Toxic and Chemistry

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“…This was, for example, the case with heavy metals in aquatic plants (Bonanno ), Gammaridae (Lieb and Carline ), fish (Campbell ), and amphibians (Priyadarshani et al. ), with pesticides in amphibian (Smalling et al. ) and damselfly (Van Praet et al.…”

Section: Impact Of Urbanization On the Biodiversity Of Urban Ponds Anmentioning

confidence: 99%

“…For example, heavy metals affect the survival, behavior, and immune system of amphibians (Priyadarshani et al. , Sievers et al. ) and increased levels of heavy metal pollution in ponds negatively influence the activity of some species of bats (Straka et al.…”

Section: Impact Of Urbanization On the Biodiversity Of Urban Ponds Anmentioning

confidence: 99%

“…Various pollutants from urban ponds have been shown to be present in organisms or even to bioaccumulate. This was, for example, the case with heavy metals in aquatic plants (Bonanno 2011), Gammaridae (Lieb and Carline 2000), fish (Campbell 1994), and amphibians (Priyadarshani et al 2015), with pesticides in amphibian (Smalling et al 2013) and damselfly (Van Praet et al 2014), and with polycyclic aromatic hydrocarbons in adult damselflies (Heintzman et al 2015).…”

Section: Water Qualitymentioning

confidence: 99%

“…These urban pollutants impact the biology of species in a variety of ways. For example, heavy metals affect the survival, behavior, and immune system of amphibians (Priyadarshani et al 2015, Sievers et al 2018b) and increased levels of heavy metal pollution in ponds negatively influence the activity of some species of bats (Straka et al 2016). Estrogen contamination linked to urbanization is also suspected of modifying sex ratios in amphibian populations (Lambert et al 2015).…”

Section: Water Qualitymentioning

confidence: 99%

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Review: Toward management of urban ponds for freshwater biodiversity

2019

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Many cities around the world are expanding and this trend in urbanization is expected to sharply increase over coming decades. At the same time, the integration of green and blue spaces is widely promoted in urban development, potentially offering numerous benefits for biodiversity. This is particularly relevant for urban waterbodies, a type of ecosystem present in most cities. However, site managers often lack the knowledge base to promote biodiversity in these waterbodies, which are generally created to provide other ecosystem services. To address this, our review presents guidelines for promoting biodiversity in urban ponds. We found a total of 516 publications indexed in ISI Web of Sciences related to this topic, of which 279 were retained for the purposes of our review. The biodiversity of urban ponds, measured by species richness, appears to be generally lower than in rural ponds; however, urban ponds often support threatened species. Furthermore, if well managed, urban ponds have the potential to support a much greater biodiversity than they currently do. Indeed, this review shows that a range of urban factors can impair or promote pond biodiversity, including many that can easily be controlled by site managers. Local factors include design (surface area, pond depth, banks and margins, shade, shoreline irregularity), water quality (conductivity, nutrients, heavy metals), and hydroperiod and biotic characteristics (stands of vegetation, fish, invasive species). Important regional factors include several indicators of urbanization (roads, buildings, density of population, impervious surfaces, car traffic), and the presence of other wetlands or green spaces in the surrounding landscape. We considered each of these factors and their potential impact on freshwater biodiversity. Taking into account the management measures listed in the publications reviewed, we have proposed a framework for the management of urban ponds, with guidelines to promote biodiversity and other ecosystem services, and to avoid ecosystem disservices or the creation of ecological traps. At the city scale, the biodiversity of a pondscape benefits from a high diversity of pond types, differing in their environmental characteristics and management.

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Is trace element concentration correlated to parasite abundance? A case study in a population of the green frog Pelophylax synkl. hispanicus from the Neto River (Calabria, southern Italy)

et al. 2017

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Bioaccumulation of 13 trace elements in the livers of 38 Pelophylax sinkl. hispanicus (Ranidae) and its helminth communities were studied and compared among three sites, each with a different degree of pollution along River Neto (south Italy) during September, 2014. Trace element concentrations in water and liver were measured using inductively coupled plasma mass spectrometry. For most elements, the highest concentration was recorded in the frogs inhabiting the third site, the one with the highest degree of pollution. The trend of trace element concentration in the liver can be represented as follows: Cu > Zn > Mn > Se > Cr. Concentrations of some elements in water and liver samples were significantly different among the three sites and this is evidenced by the bioaccumulation in the frogs. Four species of helminths, all belonging to Nematoda, were found: Rhabdias sp., Oswaldocruzia filiformis (Goeze, 1782), Cosmocerca ornata (Dujarden, 1845), Seuratascaris numidica (Seurat, 1917). The parasite survey presents an important difference of prevalence and average number of helminths in frogs between the three sites. Correlating parasitological and ecotoxicological data showed a strong positive correlation between prevalence and number of parasites with some trace elements such as Mn, Co, Ni, As, Se, and Cd.

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Heavy metal mediated immunomodulation of the Indian green frog, Euphlyctis hexadactylus (Anura:Ranidae) in urban wetlands

Cited by 36 publications

References 61 publications

Heavy metal–induced toxicity in the Indian green frog: Biochemical and histopathological alterations

Heavy metal–induced toxicity in the Indian green frog: Biochemical and histopathological alterations

Review: Toward management of urban ponds for freshwater biodiversity

Is trace element concentration correlated to parasite abundance? A case study in a population of the green frog Pelophylax synkl. hispanicus from the Neto River (Calabria, southern Italy)

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