Heavy Metal Pollution Impacts Soil Bacterial Community Structure and Antimicrobial Resistance at the Birmingham 35th Avenue Superfund Site

Goswami, Anuradha; Adkins-Jablonsky, Sarah J.; Filho, Marcelo Malisano Barreto; Shilling, Michelle D; Dawson, Alex; Heiser, Sabrina; O'Connor, Aisha; Walker, Melissa; Roberts, Qutia; Morris, Jeffrey

doi:10.1128/spectrum.02426-22

Cited by 11 publications

(2 citation statements)

References 74 publications

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“…Overall, the ambient permafrost community comprised taxa commonly found in permafrost, soil and sediments, including Chloroflexi (Solirubrobacterales and KD4-96), Actinobacteriota (Gaiellales, Micrococcales, Microtrichales), and Rhizobiales (Costello & Schmidt, 2006;Gołębiewski et al, 2014;Goswami et al, 2023;Liu et al, 2022;Zhang et al, 2019). The ambient seawater microbiome was typical for pelagic marine waters, dominated by SAR11, Flavobacteriales and Pseudomonadales (e.g., SAR86, SAR92).…”

Section: Degradation Of Ancient Organic Matter Is Mediated By Both Am...mentioning

confidence: 99%

Microbial Communities Degrade Ancient Permafrost‐Derived Organic Matter in Arctic Seawater

Ruben,

Marchant,

Wietz

et al. 2024

JGR Biogeosciences

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The Arctic is experiencing rapid warming, which among other processes results in increasing erosion of coastal permafrost and the release of ancient organic carbon (OC) into the Arctic Ocean, which in turn might result in greenhouse gas emissions following its decomposition. Supply of terrigenous organic matter to the ocean affects near‐shore nutrient concentrations and the composition of microbial communities—highlighting the need to understand the fate of permafrost‐derived carbon in this fragile ecosystem. We incubated material from coastal Yedoma permafrost for 85 days in seawater collected during the Arctic Century expedition. During this experiment, 2.8 ± 1.4% of OC from coastal Yedoma was respired to CO2. Radiocarbon analysis revealed that 88 ± 15% of the released CO2 originated from ancient material (∼40,000 years), indicating that degradation of permafrost OC reintroduces old carbon into the short‐term carbon cycle. Hence, the permafrost climate feedback may be enhanced in the coming decades when coastal erosion accelerates. Additionally, 0.9 ± 0.3% of Yedoma OC was leached as dissolved OC. The observed net production of inorganic nitrogen during the incubation could potentially provide a negative feedback by stimulating primary production. Bacterial community analysis showed a succession of primary responders to biolabile substrates (e.g., Psychrobacter and Colwellia) followed by secondary consumers of less biolabile substrates (e.g., Maribacter and Pseudohongiella), plus a potential establishment of permafrost associated‐bacteria on particles. Overall, our data show that OC input from thawing permafrost stimulates bacterial dynamics, with likely implications for regional biogeochemical cycles and the Earth's climate.

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Section: Degradation Of Ancient Organic Matter Is Mediated By Both Am...mentioning

confidence: 99%

Microbial Communities Degrade Ancient Permafrost‐Derived Organic Matter in Arctic Seawater

Ruben,

Marchant,

Wietz

et al. 2024

JGR Biogeosciences

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“…These organisms showed a propensity for soils with reduced carbon/nutrient availability (Shange et al, 2012) and are also widespread with high abundance in HM-polluted soils (Navarro-Noya et al, 2010;Gołębiewski et al, 2014;Yan et al, 2016;Salam and Varma, 2019). Recently, Goswami et al (2023) reported a positive correlation between this taxon and Zn and Pb concentration. Interestingly, the group has been identified as an indicative taxon in HM-contaminated mining and agricultural soils (Huang et al, 2021) and similarly demonstrated to associate with metal-accumulating plants and are easily enriched in HMsupplemented agricultural soils (Wu et al, 2021;Kuo et al, 2018).…”

Section: Frontiers In Environmental Sciencementioning

confidence: 99%

Geochemistry and metagenomics analyses of bacterial community structure in selected waste dumpsites in Lagos Metropolis, Nigeria

Saibu,

Adebusoye,

Oyetibo

et al. 2023

Front. Environ. Sci.

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Dumpsites are reservoirs of persistent organic pollutants (POPs) and heavy metals (HMs), constituting environmental hazards to humanity. Autochthonous microorganisms in dumpsites exhibit various degrees of responses to contaminants. Unfortunately, there is a dearth of information on the types and concentration of pollutants and the array of microorganisms in these dumpsites which may play important roles in the metabolism of such pollutants or other community processes. Therefore, determining the microbial community structure in such contaminated sites across a municipality is essential for profiling the taxa that would serve as consensus degraders of the pollutants. In this study, soil samples from three dumpsites (Cele, CS; Solous, SS; and Computer Village, CVS) were characterized for geochemical properties using GC-MS, MP-AES, and other analytical protocols, while the dynamics of bacterial communities were evaluated based on their 16S rRNA gene barcodes. A significant difference in the bacterial communities was observed among the dumpsites in relation to the extent of pollution caused by POPs and HMs. CVS, with the highest HM contamination, was rich in Actinobacteria (41.7%) and Acidobacteria (10.2%), in contrast to CS and SS. Proteobacteria (34.1%) and Firmicutes (20%) were the dominant phyla in CS (highest POP contamination), while Bacteroidetes (45.5%) and Proteobacteria (39.9%) were dominant in SS soil. Bacillus was the dominant genus in the most polluted dumpsite. Canonical correspondence analysis revealed that polycyclic aromatic hydrocarbons (PAHs) and HMs shaped the structure of the bacterial operational taxonomic units (OTUs) in the most polluted dumpsite. Out of a total of 706 OTUs, 628 OTUs exhibited a significant correlation (>50%) with benzo(b)fluoranthene, azobenzene, dibenzofurans, pyrene, dibenzo(a,l)pyrene, Cu, and Zn. In particular, Proteobacteria (Achromobacter sp. and Serratia sp.), Bacteroidetes (Zhouia sp.), and Firmicutes (Bacillus sp.) were suggested to be pivotal to the ecophysiology of dumpsite soils contaminated with POPs and HMs. The results generally underscored the importance of metagenomic and physicochemical analyses of polluted systems in enabling correlations for useful prediction of drivers of such ecosystems. This will further improve our understanding of the metabolic potential and adaptation of organisms in such systems.

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Unraveling the Role of Contaminants Reshaping the Microflora in Zea mays Seeds from Heavy Metal–Contaminated and Pristine Environment

Awais,

Xiang,

Shah

et al. 2024

Microb Ecol

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Heavy metal (HM) contaminants are the emerging driving force for reshaping the microflora of plants by eradicating the non-tolerance and non-resistant microbes via their lethal effects. Seeds served as a prime source of ancestral microbial diversity hereditary transfer from generation to generation. However, the problem arises when they got exposed to metal contamination, does metal pollutant disrupt the delicate balance of microbial communities within seeds and lead to shifts in their microflora across generations. In this study, the endophytic community within Zea mays seeds was compared across three distinct regions in Yunnan province, China: a HM-contaminated site Ayika (AK), less-contaminated site Sanduoduo (SD), and a non-contaminated Site Dali (DL). High-throughput sequencing techniques were employed to analyze the microbial communities. A total of 492,177 high-quality reads for bacterial communities and 1,001,229 optimized sequences for fungal communities were obtained. These sequences were assigned to 502 and 239 operational taxonomic units (OTUs) for bacteria and fungi, respectively. A higher diversity was recorded in AK samples than in SD and DL. Microbial community structure analysis showed higher diversity and significant fluctuation in specific taxa abundance in the metal-polluted samples exhibiting higher response of microbial flora to HM. In AK samples, bacterial genera such as Gordonia and Burkholderia-Caballeronia-Paraburkholderia were dominant, while in SD Pseudomonas and Streptomyces were dominant. Among the fungal taxa, Fusarium , Saccharomycopsis , and Lecanicillium were prevalent in HM-contaminated sites. Our finding revealed the influential effect of HM contaminants on reshaping the seed microbiome of the Zea mays , showing both the resilience of certain important microbial taxa as well the shifts in the diversity in the contaminated and pristine conditions. The knowledge will benefit to develop effective soil remediation, reclamation, and crop management techniques, and eventually assisting in the extenuation of metal pollution's adverse effects on plant health and agricultural productivity.

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Heavy Metal Pollution Impacts Soil Bacterial Community Structure and Antimicrobial Resistance at the Birmingham 35th Avenue Superfund Site

Cited by 11 publications

References 74 publications

Microbial Communities Degrade Ancient Permafrost‐Derived Organic Matter in Arctic Seawater

Microbial Communities Degrade Ancient Permafrost‐Derived Organic Matter in Arctic Seawater

Geochemistry and metagenomics analyses of bacterial community structure in selected waste dumpsites in Lagos Metropolis, Nigeria

Unraveling the Role of Contaminants Reshaping the Microflora in Zea mays Seeds from Heavy Metal–Contaminated and Pristine Environment

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