Helicobacter cinaedi meningitis: A case report and review of previous cases

Okubo, H; Goto, Masahide; Sato, Miori; Sugiyama, Teruyuki; Kawano, Motoharu; Matsunaga, Tetsuro; Akaike, Takaaki

doi:10.1016/j.jns.2014.10.011

Cited by 14 publications

(9 citation statements)

References 9 publications

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“…RPKM-based Pearson coefficients (r i,j ) were calculated for each gene-gene pair, summarized in Fig. 8 as a For several genes discussed above, napA and lctP, coexpression partners are summarized explicitly in Tables S2 and S3 T are summarized across eight culture conditions as follows (conditions 1 to 8 indicated by the numbers 1 to 8 within brackets): [1], monophasic F12, H 2 -supplemented microaerobic; [2], biphasic F12, H 2 -supplemented microaerobic; [3], biphasic F12, H 2 -free/CO 2 -supplemented microaerobic; [4], F12 plus Caco-2 cells, H 2 -supplemented microaerobic; [5], F12 plus Caco-2 cells, aerobic; [6], F12 plus 10 mM L-lactate, H 2 -supplemented microaerobic; [7], F12 plus 10 mM L-lactate, aerobic; [8], MHB, H 2 -supplemented microaerobic. Rows of the heatmap correspond to individual genes (in sequential order), while columns denote culture conditions.…”

Section: Resultsmentioning

confidence: 99%

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Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions

Schmitz

Taniguchi

Misawa

et al. 2016

Appl Environ Microbiol

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Helicobacter cinaedi is an emerging opportunistic pathogen associated with infections of diverse anatomic sites. Nevertheless, the species demonstrates fastidious axenic growth; it has been described as requiring a microaerobic atmosphere, along with a strong preference for supplemental H 2 gas. In this context, we examined the hypothesis that in vitro growth of H. cinaedi could be enhanced by coculture with human epithelial cells. When inoculated (in Ham's F12 medium) over Caco-2 monolayers, the type strain (ATCC BAA-847) gained the ability to proliferate under H 2 -free aerobic conditions. Identical results were observed during coculture with several other monolayer types (LS-174T, AGS, and HeLa). Under chemically defined conditions, 40 amino acids and carboxylates were screened for their effect on the organism's atmospheric requirements. Several molecules promoted Helicobacter cinaedi is an emerging pathogen of the class Epsilonproteobacteria. This spiral-shaped Gram-negative rod is associated with opportunistic infections of multiple anatomic sites, including the intestinal tract (1, 2), blood (3, 4), peripheral soft tissue (5, 6), and other body fluids (7-9). This behavior contrasts with related Helicobacter and Campylobacter species, which typically demonstrate a more limited tissue tropism. H. cinaedi was identified ϳ30 years ago in men who have sex with men (1) and HIV patients (10, 11), although infections have since been described in other immunocompromised groups (12, 13), as well as immunocompetent (14-17) and postsurgical patients (18).Despite abundant case reports, the ecology/epidemiology of H. cinaedi remains poorly defined and sparsely studied. Limited molecular evidence suggests that it can be found in the stools of a small percentage of healthy individuals (19). The organism has also been isolated from animal feces (including rodents, dogs, and primates), although its role as a veterinary and/or zoonotic pathogen is uncertain (20,21). An environmental reservoir has not yet been identified. The majority of recent literature on H. cinaedi (for instance, many of the current references) originates from Japan, although it is unclear whether this reflects regional prevalence or an increased awareness among Japanese investigators. Knowledge of H. cinaedi biology and tools for its genetic manipulation are likewise sparse, limited to several studies of targeted deletion mutants. These deletion mutants include strains deficient in cytolethal distending toxin (22) and alkyl hydroperoxide reductase (23), both of which demonstrate attenuated virulence in a murine colitis model.Likely contributing to this paucity of information, H. cinaedi is a challenging organism to manipulate in vitro. Strains are notable for poor, film-like growth on agar plates, a preference for biphasic culture (broth over agar), and decreased viability on subculture (24, 25). Recommended medium conditions for H. cinaedi are not

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Section: Resultsmentioning

confidence: 99%

“…This spiral-shaped Gram-negative rod is associated with opportunistic infections of multiple anatomic sites, including the intestinal tract (1, 2), blood (3,4), peripheral soft tissue (5,6), and other body fluids (7)(8)(9). This behavior contrasts with related Helicobacter and Campylobacter species, which typically demonstrate a more limited tissue tropism.…”

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confidence: 99%

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions

Schmitz

Taniguchi

Misawa

et al. 2016

Appl Environ Microbiol

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“…It is considered an “enterohepatic Helicobacter ” due to its initial isolation from the gastrointestinal tract [ 2 ]. The most common presenting symptoms of H cinaedi infection are fever and a cellulitic or nodular rash [ 3 , 4 ], although cases of myopericarditis [ 5 ], endocarditis [ 6 ], osteomyelitis [ 7 ], and meningitis [ 8 ] have been reported. Severe disseminated disease has been described in patients with or without immunocompromise.…”

Section: Discussionmentioning

confidence: 99%

Getting to the Heart of the Matter: A 20-Year-Old Man With Fever, Rash, and Chest Pain

Sabo

Boonyaratanakornkit

Cybulski

et al. 2017

Open Forum Infectious Diseases

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“…A case of H. cinaedi meningitis was reported in a 53‐year‐old man . He and his granddaughter had touched guinea pigs while visiting a zoo.…”

Section: Non‐h Pylori Helicobacter Infections In Humansmentioning

confidence: 99%

The Other Helicobacters

et al. 2015

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In the past year, a substantial number of (putative) novel Helicobacter species have been described, including Helicobacter himalayensis colonizing the Himalayan marmot and Helicobacter apodemus, colonizing the Korean striped field mouse. In addition, a putative novel gastric Helicobacter species was identified in wild gorillas and chimpanzees, for which the name "Candidatus H. homininae" was proposed. A high incidence of gastric non-H. pylori Helicobacter infection was described in China and multiple case reports have described the involvement of enterohepatic Helicobacter species, especially Helicobacter cinaedi, in a wide range of diseases. Several studies in rodent models further elucidated the mechanisms underlying the development of gastric mucosaassociated lymphoid tissue lymphoma during infection with gastric non-H. pylori Helicobacters. The effects of infection with gastric Helicobacters on the development of neuroinflammation were investigated and several enterohepatic Helicobacter species were shown to affect the composition of the gut microbiota, to influence vaccine efficiency as well as the progression of cancer in distant sites of the body. Taxonomy and PhylogenyHelicobacter himalayensis was described as a new enterohepatic Helicobacter species in 2015 [1]. H. himalayensis was isolated from gastric mucosa of Marmota himalayana in the Qinghai-Tibet Plateau in China. Analysis of the 16S rRNA gene sequence (1468 bp) showed that it was most closely related to Helicobacter marmotae (96.7% similarity), while the 23S rRNA gene sequence (2879 bp) analysis showed that it was most closely related to Helicobacter canis (96% similarity).A novel Helicobacter species was identified from the gastrointestinal tract of Korean striped field mice, for which the name Helicobacter apodemus sp. nov. was proposed [2]. The isolates were positive for urease activity and were most closely related to a reference strain of Helicobacter mesocricetorum (97.03-97.32% 16S rRNA sequence similarities).A novel Helicobacter sp. was also isolated from the cecum of the Siberian hamster [3]. Sequence analysis showed 97% sequence similarity to Helicobacter ganmani. Coinfection with Helicobacter bilis and Campylobacter spp. was observed in the Siberian hamster. Genomics and GeneticsHelicobacter suis is one of the most fastidious Helicobacter species, requiring biphasic culture conditions. Hence, cultures obtained from an individual animal may contain multiple H. suis strains. A method was developed to obtain single-colony-derived H. suis strains by culturing bacteria as colonies on 1% brucella agar plates, followed by purification and enrichment by biphasic culture [4]. However, for four of 17 strains, MLST analysis revealed genetic differences between parental and single-colony-derived strains. One colony-derived strain was further shown to have lost its capacity to colonize Mongolian gerbils.Draft genome sequences of seven enterohepatic Helicobacter species were described [5]. Six isolates (H. bilis ATCC 43879, H. canis NCTC 12...

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Helicobacter cinaedi meningitis: A case report and review of previous cases

Cited by 14 publications

References 9 publications

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions

Getting to the Heart of the Matter: A 20-Year-Old Man With Fever, Rash, and Chest Pain

The Other Helicobacters

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Helicobacter cinaedi meningitis: A case report and review of previous cases

Cited by 14 publications

References 9 publications

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H 2 -Free Aerobic Conditions

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H 2 -Free Aerobic Conditions

Getting to the Heart of the Matter: A 20-Year-Old Man With Fever, Rash, and Chest Pain

The Other Helicobacters

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Product

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Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions