Helicobacter pylori-Derived Outer Membrane Vesicles (OMVs): Role in Bacterial Pathogenesis?

Jarzab, Miroslaw; Posselt, Gernot; Meisner‐Kober, Nicole; Weßler, Silja

doi:10.3390/microorganisms8091328

Cited by 42 publications

(41 citation statements)

References 108 publications

(260 reference statements)

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“…3a). These results are confirming the findings of previous reports stating that OMVs derived from Gram-negative bacteria to be spherical bilayer membrane vesicles ranging from 20 to 500 nm [44][45][46][47][48] .…”

Section: Identification and Characterization Of Omvs Populations Derived From H Pylorisupporting

confidence: 92%

Heterogeneous Populations of Outer Membrane Vesicles Released from Helicobacter Pylori SS1 with Distinct Biological Properties

Ahmed¹,

Zheng²,

Abdalla³

et al. 2021

Eng. Sci.

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Gram-negative bacteria release outer membrane vesicles (OMVs) with great potential in a variety of biomedical applications. The study aimed to investigate nutritional growth supplements (HBS, FBS, β-Cyclodextrin, or Cholesterol) and their impact on H. pylori's OMVs biological properties. SDS-page showed a difference in protein components between all OMVs populations.Nuclear morphology for cells treated with 40 μg/mL of ExHp-CD displayed no change in nuclear morphology, while cells treated with ExHp-HBS, ExHp-FBS, and ExHp-Chlos showed chromatin condensation and nuclear fragmentation. CCK-8, LDH, and WST-1 assays showed no adverse effect for ExHp-CD in all used concentrations, while treated cells were negatively affected under higher concentrations for ExHp-HBS and ExHp-FBS (40 and 50 μg/mL) or in almost all the used concentrations for ExHp-Chlos. Obtained OMVs populations were phagocyted by macrophage RAW 264.7 cells leading to activate macrophages and produce cytokines. IL-10 and IL-4 were significantly (p<0.05) increased after stimulation with all obtained OMVs, while levels of IFN-γ and IL-12 were not significantly increased. All OMVs populations increased CD206 ratio over CD86 ratio, demonstrating M2-phenotype polarization. Current findings revealed that growth supplements influenced OMVs biological properties while inducing Th2-immune response. This knowledge could determine which type of OMVs population for the required biomedical application.

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Section: Identification and Characterization Of Omvs Populations Derived From H Pylorisupporting

confidence: 92%

Heterogeneous Populations of Outer Membrane Vesicles Released from Helicobacter Pylori SS1 with Distinct Biological Properties

Ahmed¹,

Zheng²,

Abdalla³

et al. 2021

Eng. Sci.

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“…Furthermore, the Bacteroides vulgatus strain mpk induces a tolerant semimature phenotype in BMDCs using OMVs [83] while EVs from the Bifidobacterium longum KACC 91563 strain induce the apoptosis of mast cells [84]. Helicobacter pylori OMVs are not only able to induce the release of both pro-inflammatory IL-6 and anti-inflammatory IL-10 but also induce T-cells apoptosis [85].…”

Section: Activation Of the Immune Response Of Tlrs By Microbiota-derived Evsmentioning

confidence: 99%

Role of Microbiota-Derived Extracellular Vesicles in Gut-Brain Communication

Cuesta

Guerri

Ureña

et al. 2021

IJMS

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Human intestinal microbiota comprise of a dynamic population of bacterial species and other microorganisms with the capacity to interact with the rest of the organism and strongly influence the host during homeostasis and disease. Commensal and pathogenic bacteria coexist in homeostasis with the intestinal epithelium and the gastrointestinal tract’s immune system, or GALT (gut-associated lymphoid tissue), of the host. However, a disruption to this homeostasis or dysbiosis by different factors (e.g., stress, diet, use of antibiotics, age, inflammatory processes) can cause brain dysfunction given the communication between the gut and brain. Recently, extracellular vesicles (EVs) derived from bacteria have emerged as possible carriers in gut-brain communication through the interaction of their vesicle components with immune receptors, which lead to neuroinflammatory immune response activation. This review discusses the critical role of bacterial EVs from the gut in the neuropathology of brain dysfunctions by modulating the immune response. These vesicles, which contain harmful bacterial EV contents such as lipopolysaccharide (LPS), peptidoglycans, toxins and nucleic acids, are capable of crossing tissue barriers including the blood-brain barrier and interacting with the immune receptors of glial cells (e.g., Toll-like receptors) to lead to the production of cytokines and inflammatory mediators, which can cause brain impairment and behavioral dysfunctions.

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“…Membrane vesicles are spherical structures with nanometric dimensions that transport a variety of chemicals, delivering them to the target site in an effective concentration and conditioning protection against environmental factors, such as the action of nucleases and peptidases [ 92 , 93 , 94 ]. Membrane vesicles are produced in all phases of microbial growth and secreted by both Gram-positive and Gram-negative bacteria, including H. pylori [ 95 , 96 ].…”

Section: Phenotypic Variability As a Modulator Of H Pylmentioning

confidence: 99%

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

et al. 2020

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Helicobacter pylori is a bacterium that is capable of colonizing a host for many years, often for a lifetime. The survival in the gastric environment is enabled by the production of numerous virulence factors conditioning adhesion to the mucosa surface, acquisition of nutrients, and neutralization of the immune system activity. It is increasingly recognized, however, that the adaptive mechanisms of H. pylori in the stomach may also be linked to the ability of this pathogen to form biofilms. Initially, biofilms produced by H. pylori were strongly associated by scientists with water distribution systems and considered as a survival mechanism outside the host and a source of fecal-oral infections. In the course of the last 20 years, however, this trend has changed and now the most attention is focused on the biomedical aspect of this structure and its potential contribution to the therapeutic difficulties of H. pylori. Taking into account this fact, the aim of the current review is to discuss the phenomenon of H. pylori biofilm formation and present this mechanism as a resultant of the virulence and adaptive responses of H. pylori, including morphological transformation, membrane vesicles secretion, matrix production, efflux pump activity, and intermicrobial communication. These mechanisms will be considered in the context of transcriptomic and proteomic changes in H. pylori biofilms and their modulating effect on the development of this complex structure.

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Helicobacter pylori-Derived Outer Membrane Vesicles (OMVs): Role in Bacterial Pathogenesis?

Cited by 42 publications

References 108 publications

Heterogeneous Populations of Outer Membrane Vesicles Released from Helicobacter Pylori SS1 with Distinct Biological Properties

Heterogeneous Populations of Outer Membrane Vesicles Released from Helicobacter Pylori SS1 with Distinct Biological Properties

Role of Microbiota-Derived Extracellular Vesicles in Gut-Brain Communication

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

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