Helicobacter pylori Genotypes in Russia

Momynaliev, K. Т.; Смирнова, О. В.; Kudryavtseva, Larisa Vladimirovna; Govorun, Vadim M.

doi:10.1007/s10096-003-0987-2

Cited by 5 publications

(4 citation statements)

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“…The prevalence of such mixed infections has been reported to vary (5–68%) [13]–[17] depending on geographical region, whether in a developed or developing country (low and high overall infection risk, respectively), and probably also methods of analysis. The H. pylori virulence-associated vacuolating cytotoxin ( vacA ) and cag pathogenicity island ( cag PAI) genes, and also the cag empty site in strains lacking the cag PAI, are typically found in only one copy per genome [17]–[20]. Accordingly, detection of both the cagA gene and the cag empty site, or of both s1 and s2 (signal sequence; at 5′ end of gene) or both m1 and m2 (middle region) alleles of vacA in a biopsy or in pool of H. pylori from a person indicates mixed infection.…”

Section: Introductionmentioning

confidence: 99%

Mixed Infection with cagA Positive and cagA Negative Strains of Helicobacter pylori Lowers Disease Burden in The Gambia

et al. 2011

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BackgroundThe prevalence of Helicobacter pylori including strains with putatively virulent genotypes is high, whereas the H. pylori-associated disease burden is low, in Africa compared to developed countries. In this study, we investigated the prevalence of virulence-related H. pylori genotypes and their association with gastroduodenal diseases in The Gambia.Methods and FindingsDNA extracted from biopsies and H. pylori cultures from 169 subjects with abdominal pain, dyspepsia or other gastroduodenal diseases were tested by PCR for H. pylori. The H. pylori positive samples were further tested for the cagA oncogene and vacA toxin gene.One hundred and twenty one subjects (71.6%) were H. pylori positive. The cagA gene and more toxigenic s1 and m1 alleles of the vacA gene were found in 61.2%, 76.9% and 45.5% respectively of Gambian patients harbouring H. pylori. There was a high prevalence of cagA positive strains in patients with overt gastric diseases than those with non-ulcerative dyspepsia (NUD) (p = 0.05); however, mixed infection by cagA positive and cagA negative strains was more common in patients with NUD compared to patients with gastric disease (24.5% versus 0%; p = 0.002).ConclusionThis study shows that the prevalence of H. pylori is high in dyspeptic patients in The Gambia and that many strains are of the putatively more virulent cagA+, vacAs1 and vacAm1 genotypes. This study has also shown significantly lower disease burden in Gambians infected with a mixture of cag-positive and cag-negative strains, relative to those containing only cag-positive or only cag-negative strains, which suggests that harbouring both cag-positive and cag-negative strains is protective.

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Section: Introductionmentioning

confidence: 99%

Mixed Infection with cagA Positive and cagA Negative Strains of Helicobacter pylori Lowers Disease Burden in The Gambia

et al. 2011

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“…The presence of the cagA gene (a marker of the pathogenicity island, cagPAI) varies among H. pylori strains of different geographical origin: ~ 80-99% in East Asian countries [14,21], Southeast and South Asia [20,22,27], South Africa [24]; ~ 50-70% in countries of Western Europe [7,12,15,17]; ~ 50% and lower in the countries of the Middle East [10,19]. According to the studies conducted in the Russian Federation, the presence of cagA-positive H. pylori strains varies in different regions: 80-90% in Moscow (Central region) [18] and Yekaterinburg (Ural Federal District) [3], 70-80% in Rostov-on-Don, Astrakhan (Southern Federal District) [2], 30-60% in Eastern Siberia [25], < 50% in Kazan (Volga Federal District) [1].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, a strong association between cagA status and severity of the disease was reported in the developed European countries [15]. In Russia and most Asian countries, such contribution was not proved [18,21]. The cagPAI genes encode for the type IV secretion system proteins that transport the immunogenic CagA protein to the epithelial cells of the gastric mucosa.…”

Section: Introductionmentioning

confidence: 99%

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Genetic polymorphisms of helicobacter pylori clinical isolates in St. Petersburg, Russia

Сварваль

Старкова

Ферман

et al. 2022

Russian Journal of Infection and Immunity

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Introduction: Helicobacter pylori was proved to be the principal causative agent of gastroduodenal disorders in human. Although Russian Federation is among the countries with a high prevalence of H. pylori infection (60-90%), there is currently a very limited number of studies evaluating H. pylori genotypes in Russia. Objective: Based on the assessment of virulence-associated cagA, oipA, and vacA genes, we aimed to determine H. pylori genotypes associated with the clinical outcomes in patients with H. pylori infection in St. Petersburg, North-West Russia. Methodology: Using PCR for the detection of cagA, oipA, and vacA s-, m-, i- allelic variants, we analyzed 61 H. pylori isolates isolated and cultured from biopsies collected during endoscopy of patients with chronic gastritis (G), duodenal ulcer (DU), and gastric cancer (GC). Results: The genetic diversity of H. pylori clinical isolates has been revealed (HGDI 0.88): 41 (67%) of 61 H. pylori isolates were cagA-positive, 38 (62%) - oipA-positive. The proportions of cagA+ isolates differed in patients with G (56.7%) and DU (80.9%), (p=0.06). The s, m, and i allelic variants of the vacA gene were detected in all strains, although the vacA s1 allele was significantly dominant in patients with DU (95.2%) rather than with G (64.9%), (p=0.01). The vacA alleles m1 and i1 in the isolates from patients with G and DU were found in almost equal proportions: 45.9% and 42.8% for m1 allele, 45.9% and 47.6% for i1 allele, respectively. Seven isolates (11.5%) were positive for different mixed combinations of vacA alleles s, m, and i. Noteworthy, all vacA s2 strains were cagA-negative and had the m2 allele. OipA+ strains were found in almost equal proportions in patients with G (62.2%) and DU (57.1%), p=0.71. All three cagA- and oipA- positive isolates from patients with GC carried vacA s1/m1/i1 alleles. Different combinations of virulence-associated determinants constituted 17 genetic profiles. The most common combined genotype cagA+/oipA+/vacAs1/m1/i1 comprised 18 (29.5%) H. pylori isolates. Conclusion: We have determined predominant genotypes in the H. pylori population in North-West Russia. The significant association between vacAs1 genotype of the pathogen and clinical manifestations of H. pylori infection has been established in our study.

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Peculiarities of polymorphism of Helicobacter pylori virulence genes and IL-1 genes in peptic ulcer of stomach and duodenum, associated with Helicobacter pylori

Tchernova¹,

Nasybullina²,

Gorshkov³

et al. 2005

Bûll. sib. med.

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Helicobacter pylori Genotypes in Russia

Cited by 5 publications

References 5 publications

Mixed Infection with cagA Positive and cagA Negative Strains of Helicobacter pylori Lowers Disease Burden in The Gambia

Mixed Infection with cagA Positive and cagA Negative Strains of Helicobacter pylori Lowers Disease Burden in The Gambia

Genetic polymorphisms of <i>helicobacter pylori</i> clinical isolates in St. Petersburg, Russia

Peculiarities of polymorphism of <i>Helicobacter pylori</i> virulence genes and IL-1 genes in peptic ulcer of stomach and duodenum, associated with <i>Helicobacter pylori</i>

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