Helicobacter pylori in patients can be killed by visible light

Ganz, Robert A.; Viveiros, Jennifer; Ahmad, Aamir; Ahmadi, Atosa; Khalil, Ayesha; Tolkoff, M. Joshua; Nishioka, Norman S.; Hamblin, Michael R.

doi:10.1002/lsm.20161

Cited by 121 publications

(100 citation statements)

References 30 publications

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“…It was stated in previous studies that phototoxicity in the presence of exogenous photosensitizers such as Rose Bengal, Erythrosine, Toluidine blue, Methylene blue and many other photosensitizers increases upon light irradiation, caused by a series of energy transfers from light energy to molecular energy, thereby generating ROS and singlet oxygen causing cytotoxicity to the bacterial cells [1,11,13,30]. Studies also indicate that the presence of endogenous bacterial porphyrins act as photosensitizers causing bacterial cell death due to similar photochemical reactions [3,23,42,44].…”

Section: Discussionmentioning

confidence: 96%

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Photo Inactivation of Streptococcus mutans Biofilm by Violet-Blue light

et al. 2016

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Among various preventive approaches, non-invasive phototherapy/photodynamic therapy is one method used to control oral biofilm. Studies indicate that light at specific wavelengths has a potent antibacterial effect. The objective of this study was to determine the effectiveness of violet-blue light at 380-440 nm to inhibit biofilm formation of Streptococcus mutans or kill S. mutans. S. mutans UA159 biofilm cells were grown for 12-16 h in 96-well flat-bottom microtiter plates using Tryptic Soy broth (TSB) or TSB with 1% sucrose (TSBS). Biofilm was irradiated with violet-blue light for 5 min. After exposure, plates were re-incubated at 37°C for either 2 or 6 h to allow the bacteria to recover. A crystal violet biofilm assay was used to determine relative densities of the biofilm cells grown in TSB, but not in TSBS, exposed to violet-blue light. The results indicated a statistically significant (p < 0.05) decrease compared to the nontreated groups after the 2 or 6h recovery period. Growth rates of planktonic and biofilm cells indicated a significant reduction in the growth rate of the violet-blue light-treated groups grown in TSB and TSBS. Biofilm viability assays confirmed a statistically significant difference between Violet-blue light-treated and non-treated groups in TSB and TSBS. Visible Violet-blue light of the electromagnetic spectrum has the ability to inhibit S. mutans growth and reduce the formation of S. mutans biofilm. This in-vitro study demonstrated that Violet-blue light has the capacity to inhibit S. mutans biofilm formation. Potential clinical applications of light therapy in the future remain bright in preventing the development and progression of dental caries.

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Section: Discussionmentioning

confidence: 96%

“…Previous studies demonstrated that blue light within a specific wavelength or range of wavelengths has a potent antibacterial effect [2,8,11,13,21,22,29]. In the 1990's, research focused on photodynamic therapy employing photosensitizers to enhance the killing of oral bacteria.…”

Section: Introductionmentioning

confidence: 99%

Photo Inactivation of Streptococcus mutans Biofilm by Violet-Blue light

et al. 2016

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“…Accordingly, laser irradiation with blue light (~405 nm) without prior photosensitization of cultured H. pylori is sufficient to induce cell death [185]. A clinical study using 405-nm endoscopic light confirmed APDT efficacy in eradicating H. pylori colonies in patients [186]. In this small scale pilot study, 9 eligible patients received endoscopic blue light at a fluence of 40 J/cm 2 .…”

Section: Helicobacter Pylori (Gram-negative)mentioning

confidence: 90%

Antibacterial photodynamic therapy: overview of a promising approach to fight antibiotic-resistant bacterial infections

et al. 2015

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Antibacterial photodynamic therapy (APDT) has drawn increasing attention from the scientific society for its potential to effectively kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance that bacteria can rapidly develop against traditional antibiotic therapy. The review summarizes the mechanism of action of APDT, the photosensitizers, the barriers to PS localization, the targets, the in vitro-, in vivo-, and clinical evidence, the current developments in terms of treating Gram-positive and Gram-negative bacteria, the limitations, as well as future perspectives. Relevance for patients: A structured overview of all important aspects of APDT is provided in the context of resistant bacterial species. The information presented is relevant and accessible for scientists as well as clinicians, whose joint effort is required to ensure that this technology benefits patients in the post-antibiotic era.

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“…146 This is actually also true in H. pylori infected patients, whom the application of 405 nm endoscopic light alone was capable of reducing Colony Forming Unit (CFU) counts by about 90%. 147 PDT's very nature makes it ideal for the treatment of skin, wound and burn infections, all of which are easily accessible for light therapies. [148][149][150] XF73, a cationic porphyrin PS, is able to reduce MRSA growth by >3 log 10 in a porcine skin infection model.…”

Section: Acknowledgmentsmentioning

confidence: 99%