Helicobacteriose em leitões: imuno-histoquímica em amostras colhidas por meio de gastroscopia

Silveira, Renato Luiz; Cruz, A.C.M; Weber, Florian; Degani, Viviane Alexandre Nunes; Abílio, Edmundo Jorge; Carvalho, Eulógio Carlos Queiróz de

doi:10.1590/1678-7251

Cited by 2 publications

(7 citation statements)

References 16 publications

(13 reference statements)

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“…In an endoscopic Regarding the immunohistochemical analysis, eight animals (20%) were negative in all regions, while 32 (80%) were positive in at least one, but only one animal (2.5%) was positive in all of them. SZEREDI et al (2005) reported 85.4% positivity in all regions in their studies, while SILVEIRA et al (2014) reported 50% negative in all regions, and 50% positive in at least one of the regions. Regarding the antrum region, 10 (25%) were positive, and 30 (75%) were negative.…”

Section: Resultsmentioning

confidence: 87%

“…For the region of the fundic gland, three (7.5%) were positive, and 37 (92.5%) were negative (Table 2). SILVEIRA et al (2014), in an endoscopic study with piglets, reported 10 (50%) positives in the antrum region, two (10%) positives in the aglandular region, three (15%) positives in the region of the cardiac gland, and two (10%) positives in the region of the fundic gland. According to these results, the impact of bacterial infection on the stomach and on the health of herds associated with the potential to cause disease in humans, justifies the interest of further studies on the subject (TAMIASSO et al, 2017).…”

Section: Resultsmentioning

confidence: 95%

“…For this study, samples of formalin-fixed paraffin-embedded gastric tissues from 40 minipigs were used (SILVEIRA et al, 2015). The piglets had an average weight of 39.0 Kg and an average age of 17 months, were kept in the colony of the UENF Veterinary Hospital under constant temperature and humidity conditions, and fed twice a day.…”

Section: Methodsmentioning

confidence: 99%

“…Aglandular region integrity has been observed in macroscopic evaluation. The ulcerative lesions were classified using the scoring method proposed by SILVEIRA et al (2014), from zero to five: assigning the score 0 to a normal appearance (smooth, glossy and whitish); score 1 to those with that were partially rough looking, with any yellow pigmentation; score 2 to the ones with fully rough and thickened areas of yellow pigmentation; score 3 to totally rough and thickened aglandular areas associated with yellow pigmentation of the entire surface; score 4 to the ones with totally rough and thickened areas, with the entire surface covered in yellow pigmentation, and eroded areas; and score 5 to those with a rough and thickened appearance, yellow pigmentation on its entire surface, and ulceration areas.…”

Section: Methodsmentioning

confidence: 99%

“…is also used for diagnosis in other species at the UENF university hospital. Samples previously tested were used for the positive and negative controls (SILVEIRA et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

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Helicobacter spp. and gastric lesions association in minipigs

et al. 2021

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Due to their similarity to humans, studies regarding gastric ulcers in pigs have become of great interest. It is known that Helicobacter spp. is related to the occurrence of these ulcers, as they possess a high prevalence in pigs. This study aimed to associate gastric lesions findings naturally occurring in minipigs related to or not related to the presence of Helicobacter spp., through the ultra-rapid urease test and immunohistochemical analysis, reaffirming the disease in swine as a natural biomedical model for human cases. For this, samples of formalin-fixed paraffin-embedded gastric tissues from 40 minipigs were obtained at the Laboratório de Morfologia e Patologia Animal (LMPA) in the Universidade Estadual do Norte Fluminense (UENF). Regarding the ultra-rapid urease test, no animal was positive in all regions. However, 18 were positive in at least one: six (15%) were positive in the aglandular region, eight (20%) in the antrum region, 13 (32.5%) in the region of the cardiac gland, and one (2.5%) in the region of the fundic gland. Regarding immunohistochemical analysis, only one animal was positive in all regions, and 32 animals were positive in at least one: four (10%) were positive in the aglandular region, 10 (25%) in the antrum region, 23 (57.5%) in the region of the cardiac gland, and three (7.5%) in the region of the fundic gland. The gastric lesion findings showed a close relationship with Helicobacter spp., enriching the laboratory animal pathologies list. The immunostaining of the bacteria not associated with gastric lesions in certain regions demonstrated the saprophytic and opportunisitic nature of Helicobacter.

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…is also used for diagnosis in other species at the UENF university hospital. Samples previously tested were used for the positive and negative controls (SILVEIRA et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

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Helicobacter spp. and gastric lesions association in minipigs

et al. 2021

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Lesões pré-ulcerativas gastroesofágicas em miniporcos Gastroesophageal ulcer research in mini pigs

Silveira¹,

Cruz²,

Medina³

et al. 2015

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A úlcera gastroesofágica figura como uma das principais causas de morte súbita de suínos em fase de reprodução. O objetivo deste trabalho foi avaliar os achados anatomopatológicos das lesões gástricas subclínicas em miniporcos. Quarenta animais, com peso médio de 39kg e idade de 17 meses, foram mantidos em biotério, em condições de temperatura e umidade constantes, alimentados duas vezes ao dia, sendo depois eutanasiados e necropsiados. Os estômagos foram colhidos e avaliados. Macroscopicamente, 29 animais apresentaram alterações na região aglandular, sendo que 37,5% foram classificadas no grau 1; 22,5%, no grau 2; e 12,5%, no grau 3. Microscopicamente, 29 (72,5%) miniporcos apresentaram paraqueratose. Destes, 35% tinham a forma moderada, 25% a discreta e 12,5% a acentuada. As lesões glandulares foram mais extensas na região cárdica, seguida da antral e fúndica. Trinta e seis (90%) animais apresentaram lesão em pelo menos uma das três regiões. Em 21 (52,5%) mini-porcos observou-se hiperatividade mucípara com catarro; em 17 (42,5%), aumento da atividade mucípara; em dez (25%), erosão. Os achados revelaram alta prevalência de lesões pré-ulcerativas nos estômagos dos miniporcos. A presença destas relacionou-se à gravidade macroscópica: quanto mais graves, mais intensa a paraqueratose.

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Helicobacteriose em leitões: imuno-histoquímica em amostras colhidas por meio de gastroscopia

Cited by 2 publications

References 16 publications

Helicobacter spp. and gastric lesions association in minipigs

Helicobacter spp. and gastric lesions association in minipigs

Lesões pré-ulcerativas gastroesofágicas em miniporcos Gastroesophageal ulcer research in mini pigs

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