Helix loop-mediated isothermal amplification of nucleic acids

Mao, Rui; Qi, Lifei; Wang, Zhuo; Liu, Hongtao; Du, Yuguang

doi:10.1039/c8ra01201f

Cited by 7 publications

(1 citation statement)

References 18 publications

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“…Nucleic acid testing consists of three main steps: nucleic acid extraction [34][35][36][37][38][39][40][41][42][43][44], nucleic acid amplification [45][46][47][48][49][50][51][52][53][54][55][56][57], and detection methods [58][59][60][61][62]. The general procedure for nucleic acid extraction involves collecting the sample to be tested (such as oropharyngeal swabs, fingertip blood, or saliva) and then obtaining the nucleic acid through a process of lysis, separation, precipitation, and purification [63][64][65].…”

Section: Introductionmentioning

confidence: 99%

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

Wang,

Zhou

et al. 2023

Biosensors

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Pathogenic pathogens invade the human body through various pathways, causing damage to host cells, tissues, and their functions, ultimately leading to the development of diseases and posing a threat to human health. The rapid and accurate detection of pathogenic pathogens in humans is crucial and pressing. Nucleic acid detection offers advantages such as higher sensitivity, accuracy, and specificity compared to antibody and antigen detection methods. However, conventional nucleic acid testing is time-consuming, labor-intensive, and requires sophisticated equipment and specialized medical personnel. Therefore, this review focuses on advanced nucleic acid testing systems that aim to address the issues of testing time, portability, degree of automation, and cross-contamination. These systems include extraction-free rapid nucleic acid testing, fully automated extraction, amplification, and detection, as well as fully enclosed testing and commercial nucleic acid testing equipment. Additionally, the biochemical methods used for extraction, amplification, and detection in nucleic acid testing are briefly described. We hope that this review will inspire further research and the development of more suitable extraction-free reagents and fully automated testing devices for rapid, point-of-care diagnostics.

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